Thylakoid membranes can be immobilized to enhance their stability in photoelectrochemical cells exposed to strong continuous illumination. This paper studies the effect of such immobilization in a glutaraldehyde-albumin crosslinked matrix on the rates of photoinhibition of electron transfer and chlorophyll photobleaching. The immobilization matrix constituted an efficient oxygen diffusion barrier that prevents chlorophyll photobleaching to a great extent. The photoprotection was less efficient against photoinhibition as seen by monitoring oxygen evolution after given periods of preillumination in the cell of a photoacoustic spectrophotometer. The results also indicated that light dispersion by the immobilization matrix was not a critical factor for photoprotection.
- chloroplast membranes
- electron transfer
- photoacoustic spectroscopy
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology