Photodynamic treatment of lens epithelial cells for cataract surgery

Robert W. Lingua, Jean Marie Parel, Gabriel Simon, Kam Li

Research output: Contribution to journalConference article

3 Scopus citations

Abstract

Photodynamic therapy (PDT) employing Dihematoporphyrin ethers (DHE) (Photofrin II) at pharmacologic levels, has been demonstrated to kill rabbit lens epithelial cells, in vivo. This in vitro study, reports on the minimal necessary parameters for rabbit lens epithelial cell death. Explants of rabbit lenses were incubated in various concentrations of DHE (10, 50, 100, 500, 1000 ug/ml) for 1, 2, or 5 minutes. 30 to 120 Joules/cm2 of collimated 514.5 nm Argon laser light were delivered to the lower concentrations of 10, 50, and 100 ug/ml DHE treated cells. One hundred fifteen explants were treated, in all. Higher concentrations of DHE alone (500 and 1000 ug/ml) were sufficient to induce cellular swelling. Lower concentrations required light for cellular effect. Trypan blue staining revealed cell death at these minimal parameters: DHE 50 ug/ml, incubation 1 minute, 514.5 nm Argon light 1.0 Watt/cm2 for 30 sec (30 Joules). In future studies, these parameters will be tested in vivo, for their ability to eliminate lens epithelial proliferation after cataract surgery.

Original languageEnglish (US)
Pages (from-to)58-61
Number of pages4
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1423
StatePublished - Jan 1 1991
EventProceedings of Ophthalmic Technologies - Los Angeles, CA, USA
Duration: Jan 21 1991Jan 22 1991

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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