Photodynamic treatment of lens epithelial cells for cataract surgery

Robert W. Lingua, Jean-Marie A Parel, Gabriel Simon, Kam Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Photodynamic therapy (PDT) employing Dihematoporphyrin ethers (DHE) (Photofrin II) at pharmacologic levels, has been demonstrated to kill rabbit lens epithelial cells, in vivo. This in vitro study, reports on the minimal necessary parameters for rabbit lens epithelial cell death. Explants of rabbit lenses were incubated in various concentrations of DHE (10, 50, 100, 500, 1000 ug/ml) for 1, 2, or 5 minutes. 30 to 120 Joules/cm 2 of collimated 514.5 nm Argon laser light were delivered to the lower concentrations of 10, 50, and 100 ug/ml DHE treated cells. One hundred fifteen explants were treated, in all. Higher concentrations of DHE alone (500 and 1000 ug/ml) were sufficient to induce cellular swelling. Lower concentrations required light for cellular effect. Trypan blue staining revealed cell death at these minimal parameters: DHE 50 ug/ml, incubation 1 minute, 514.5 nm Argon light 1.0 Watt/cm 2 for 30 sec (30 Joules). In future studies, these parameters will be tested in vivo, for their ability to eliminate lens epithelial proliferation after cataract surgery.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsCarmen A. Puliafito
PublisherPubl by Int Soc for Optical Engineering
Pages58-61
Number of pages4
Volume1423
StatePublished - 1991
EventProceedings of Ophthalmic Technologies - Los Angeles, CA, USA
Duration: Jan 21 1991Jan 22 1991

Other

OtherProceedings of Ophthalmic Technologies
CityLos Angeles, CA, USA
Period1/21/911/22/91

Fingerprint

cataracts
surgery
Surgery
Ethers
Lenses
ethers
lenses
rabbits
Cell death
death
low concentrations
Argon lasers
argon lasers
Photodynamic therapy
staining
swelling
Swelling
Argon
therapy
Cells

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Lingua, R. W., Parel, J-M. A., Simon, G., & Li, K. (1991). Photodynamic treatment of lens epithelial cells for cataract surgery. In C. A. Puliafito (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1423, pp. 58-61). Publ by Int Soc for Optical Engineering.

Photodynamic treatment of lens epithelial cells for cataract surgery. / Lingua, Robert W.; Parel, Jean-Marie A; Simon, Gabriel; Li, Kam.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Carmen A. Puliafito. Vol. 1423 Publ by Int Soc for Optical Engineering, 1991. p. 58-61.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lingua, RW, Parel, J-MA, Simon, G & Li, K 1991, Photodynamic treatment of lens epithelial cells for cataract surgery. in CA Puliafito (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 1423, Publ by Int Soc for Optical Engineering, pp. 58-61, Proceedings of Ophthalmic Technologies, Los Angeles, CA, USA, 1/21/91.
Lingua RW, Parel J-MA, Simon G, Li K. Photodynamic treatment of lens epithelial cells for cataract surgery. In Puliafito CA, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1423. Publ by Int Soc for Optical Engineering. 1991. p. 58-61
Lingua, Robert W. ; Parel, Jean-Marie A ; Simon, Gabriel ; Li, Kam. / Photodynamic treatment of lens epithelial cells for cataract surgery. Proceedings of SPIE - The International Society for Optical Engineering. editor / Carmen A. Puliafito. Vol. 1423 Publ by Int Soc for Optical Engineering, 1991. pp. 58-61
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