Photochemically induced spinal cord injury in the rat

Brant D. Watson, Ricardo Prado, W. Dalton Dietrich, Myron Ginsberg, Barth A Green

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

We have developed in the rat a minimally invasive model of reproducible spinal cord injury initiated photochemically. With the exposed spinal column intact, 560 nm irradiation of the translucent dorsal surface induces excitation of the systemically injected dye, rose Bengal, in the spinal cord microvasculature. The resultant photochemical reaction leads to vascular stasis. Histopathological changes at 7 days include hemorrhagic necrosis of the central gray matter, edematous pale-staining white matter tracts and vascular congestion. At the level of cord irradiation (T8) the entire cord thickness is necrosed except for the periphery of the anterior funiculus. Voluntary motor function is consistently lost in the subacute phase of injury.

Original languageEnglish
Pages (from-to)296-300
Number of pages5
JournalBrain Research
Volume367
Issue number1-2
DOIs
StatePublished - Mar 5 1986

Fingerprint

Spinal Cord Injuries
Blood Vessels
Rose Bengal
Microvessels
Spinal Cord
Spine
Necrosis
Coloring Agents
Staining and Labeling
Wounds and Injuries
White Matter
Gray Matter

Keywords

  • infarction
  • microvascular stasis
  • photochemical sensitization
  • singlet oxygen
  • spinal cord injury

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Photochemically induced spinal cord injury in the rat. / Watson, Brant D.; Prado, Ricardo; Dalton Dietrich, W.; Ginsberg, Myron; Green, Barth A.

In: Brain Research, Vol. 367, No. 1-2, 05.03.1986, p. 296-300.

Research output: Contribution to journalArticle

Watson, Brant D. ; Prado, Ricardo ; Dalton Dietrich, W. ; Ginsberg, Myron ; Green, Barth A. / Photochemically induced spinal cord injury in the rat. In: Brain Research. 1986 ; Vol. 367, No. 1-2. pp. 296-300.
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