Photochemically induced spinal cord injury in the rat

Brant D. Watson; Ricardo Prado; W. Dalton Dietrich; Myron D. Ginsberg; Barth A. Green

doi:10.1016/0006-8993(86)91606-9

Photochemically induced spinal cord injury in the rat

Brant D. Watson, Ricardo Prado, W. Dalton Dietrich, Myron D. Ginsberg, Barth A. Green

Research output: Contribution to journal › Article

87 Scopus citations

Abstract

We have developed in the rat a minimally invasive model of reproducible spinal cord injury initiated photochemically. With the exposed spinal column intact, 560 nm irradiation of the translucent dorsal surface induces excitation of the systemically injected dye, rose Bengal, in the spinal cord microvasculature. The resultant photochemical reaction leads to vascular stasis. Histopathological changes at 7 days include hemorrhagic necrosis of the central gray matter, edematous pale-staining white matter tracts and vascular congestion. At the level of cord irradiation (T8) the entire cord thickness is necrosed except for the periphery of the anterior funiculus. Voluntary motor function is consistently lost in the subacute phase of injury.

Original language	English (US)
Pages (from-to)	296-300
Number of pages	5
Journal	Brain Research
Volume	367
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(86)91606-9
State	Published - Mar 5 1986

Fingerprint

Keywords

infarction
microvascular stasis
photochemical sensitization
singlet oxygen
spinal cord injury

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

Cite this

Watson, B. D., Prado, R., Dalton Dietrich, W., Ginsberg, M. D., & Green, B. A. (1986). Photochemically induced spinal cord injury in the rat. Brain Research, 367(1-2), 296-300. https://doi.org/10.1016/0006-8993(86)91606-9

@article{ddbf123afb69459f81e736703ea5a91d,

title = "Photochemically induced spinal cord injury in the rat",

abstract = "We have developed in the rat a minimally invasive model of reproducible spinal cord injury initiated photochemically. With the exposed spinal column intact, 560 nm irradiation of the translucent dorsal surface induces excitation of the systemically injected dye, rose Bengal, in the spinal cord microvasculature. The resultant photochemical reaction leads to vascular stasis. Histopathological changes at 7 days include hemorrhagic necrosis of the central gray matter, edematous pale-staining white matter tracts and vascular congestion. At the level of cord irradiation (T8) the entire cord thickness is necrosed except for the periphery of the anterior funiculus. Voluntary motor function is consistently lost in the subacute phase of injury.",

keywords = "infarction, microvascular stasis, photochemical sensitization, singlet oxygen, spinal cord injury",

author = "Watson, {Brant D.} and Ricardo Prado and {Dalton Dietrich}, W. and Ginsberg, {Myron D.} and Green, {Barth A.}",

year = "1986",

month = mar,

day = "5",

doi = "10.1016/0006-8993(86)91606-9",

language = "English (US)",

volume = "367",

pages = "296--300",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Photochemically induced spinal cord injury in the rat

AU - Watson, Brant D.

AU - Prado, Ricardo

AU - Dalton Dietrich, W.

AU - Ginsberg, Myron D.

AU - Green, Barth A.

PY - 1986/3/5

Y1 - 1986/3/5

N2 - We have developed in the rat a minimally invasive model of reproducible spinal cord injury initiated photochemically. With the exposed spinal column intact, 560 nm irradiation of the translucent dorsal surface induces excitation of the systemically injected dye, rose Bengal, in the spinal cord microvasculature. The resultant photochemical reaction leads to vascular stasis. Histopathological changes at 7 days include hemorrhagic necrosis of the central gray matter, edematous pale-staining white matter tracts and vascular congestion. At the level of cord irradiation (T8) the entire cord thickness is necrosed except for the periphery of the anterior funiculus. Voluntary motor function is consistently lost in the subacute phase of injury.

AB - We have developed in the rat a minimally invasive model of reproducible spinal cord injury initiated photochemically. With the exposed spinal column intact, 560 nm irradiation of the translucent dorsal surface induces excitation of the systemically injected dye, rose Bengal, in the spinal cord microvasculature. The resultant photochemical reaction leads to vascular stasis. Histopathological changes at 7 days include hemorrhagic necrosis of the central gray matter, edematous pale-staining white matter tracts and vascular congestion. At the level of cord irradiation (T8) the entire cord thickness is necrosed except for the periphery of the anterior funiculus. Voluntary motor function is consistently lost in the subacute phase of injury.

KW - infarction

KW - microvascular stasis

KW - photochemical sensitization

KW - singlet oxygen

KW - spinal cord injury

UR - http://www.scopus.com/inward/record.url?scp=0022457730&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022457730&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(86)91606-9

DO - 10.1016/0006-8993(86)91606-9

M3 - Article

C2 - 3697704

AN - SCOPUS:0022457730

VL - 367

SP - 296

EP - 300

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -

Access to Document

10.1016/0006-8993(86)91606-9