Photochemically induced cystic lesion in the rat spinal cord. I. Behavioral and morphological analysis

Tracy Cameron, Ricardo Prado, Brant D. Watson, Mayra Gonzalez-Carvajal, Vicky R. Holets

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The present study describes the production of a spinal cord lesion which is initiated by vascular occlusion resulting from the interaction between the photosensitizing dye erythrosin B and an argon laser beam. The lesion has characteristics similar to those of the central cavity thought to lead to the production of post-traumatic syringomyelia (PTS) in humans. The present study examines the behavioral and morphological characteristics of this injury over a 28-day period. Histological analysis revealed a cavity extending from the dorsal horns to lamina VIII, with some lateral and ventral pathways being spared. The cavity volume reached a maximum 7 days after lesion induction. Behavioral changes were assessed using six different tests of motor and reflex function (motor function, climbing, waterbath, inclined plane, withdrawal to pain, and withdrawal to extension). Lesioned animals exhibited flaccid paralysis for 3-5 days, which resolved afterward. The photochemically induced cavity should provide a reproducible model for examining the effects of cystic spinal cord injury on locomotor and reflex function.

Original languageEnglish
Pages (from-to)214-223
Number of pages10
JournalExperimental Neurology
Volume109
Issue number2
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Fingerprint

Reflex
Spinal Cord
Erythrosine
Syringomyelia
Argon
Spinal Cord Injuries
Paralysis
Blood Vessels
Lasers
Coloring Agents
Pain
Wounds and Injuries
Spinal Cord Dorsal Horn
Spinal Cord Ventral Horn

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

Cite this

Photochemically induced cystic lesion in the rat spinal cord. I. Behavioral and morphological analysis. / Cameron, Tracy; Prado, Ricardo; Watson, Brant D.; Gonzalez-Carvajal, Mayra; Holets, Vicky R.

In: Experimental Neurology, Vol. 109, No. 2, 01.01.1990, p. 214-223.

Research output: Contribution to journalArticle

Cameron, Tracy ; Prado, Ricardo ; Watson, Brant D. ; Gonzalez-Carvajal, Mayra ; Holets, Vicky R. / Photochemically induced cystic lesion in the rat spinal cord. I. Behavioral and morphological analysis. In: Experimental Neurology. 1990 ; Vol. 109, No. 2. pp. 214-223.
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