The non-radiative decay of an excited chromophore can be measured by photoacoustic spectroscopy, but the application of this technique to the study of visual pigments is complicated by the light-sensitivity of the rhodopsin molecule. We succeeded in measuring photoacoustic spectra of native visual pigment. Our results provide experimental evidence that the internal conversion process is an important deactivation pathway in the early intermediates of rhodopsin's bleaching cycle. Moreover, the particular detection system of photoacoustic spectroscopy makes possible measurements on a whole retina.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|State||Published - May 15 1981|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology