Photoacoustic spectroscopy of cattle visual pigment at low temperature

François Boucher, Roger Leblanc

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The non-radiative decay of an excited chromophore can be measured by photoacoustic spectroscopy, but the application of this technique to the study of visual pigments is complicated by the light-sensitivity of the rhodopsin molecule. We succeeded in measuring photoacoustic spectra of native visual pigment. Our results provide experimental evidence that the internal conversion process is an important deactivation pathway in the early intermediates of rhodopsin's bleaching cycle. Moreover, the particular detection system of photoacoustic spectroscopy makes possible measurements on a whole retina.

Original languageEnglish
Pages (from-to)385-390
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume100
Issue number1
DOIs
StatePublished - May 15 1981
Externally publishedYes

Fingerprint

Photoacoustic spectroscopy
Retinal Pigments
Rhodopsin
Spectrum Analysis
Photophobia
Temperature
Photosensitivity
Photoacoustic effect
Chromophores
Bleaching
Retina
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Photoacoustic spectroscopy of cattle visual pigment at low temperature. / Boucher, François; Leblanc, Roger.

In: Biochemical and Biophysical Research Communications, Vol. 100, No. 1, 15.05.1981, p. 385-390.

Research output: Contribution to journalArticle

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