Photoacoustic spectroscopy of cattle visual pigment at low temperature

François Boucher, Roger M. Leblanc

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The non-radiative decay of an excited chromophore can be measured by photoacoustic spectroscopy, but the application of this technique to the study of visual pigments is complicated by the light-sensitivity of the rhodopsin molecule. We succeeded in measuring photoacoustic spectra of native visual pigment. Our results provide experimental evidence that the internal conversion process is an important deactivation pathway in the early intermediates of rhodopsin's bleaching cycle. Moreover, the particular detection system of photoacoustic spectroscopy makes possible measurements on a whole retina.

Original languageEnglish (US)
Pages (from-to)385-390
Number of pages6
JournalBiochemical and biophysical research communications
Volume100
Issue number1
DOIs
StatePublished - May 15 1981
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this