Photoacoustic spectroscopy of Anacystis nidulans. I. Effect of sample thickness on the photoacoustic signal

Robert Carpentier, Bernard Larue, Roger M. Leblanc

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

A photoacoustic spectroscopy study of the cyanobacteria Anacystis nidulans has been undertaken. It is demonstrated, by using a filter deposition technique, that the photoacoustic signal intensity becomes progressively saturated as the thickness of the algal layer is increased. This saturation effect originates mostly from the limited optical penetration of the sample and distorts the photoacoustic spectrum from its true shape. A theoretical model is proposed to explain these results, and practical means to obviate the limitations of this spectroscopic technique are suggested.

Original languageEnglish (US)
Pages (from-to)403-410
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume222
Issue number2
DOIs
StatePublished - Apr 15 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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