Photoacoustic detection of photosynthetic energy storage in Photosystem II submembrane fractions

Robert Carpentier, Roger M. Leblanc, Murielle Mimeault

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Photoacoustic spectroscopy can be used for the measurement of energy storage during photosynthesis. We report here the monitoring of photosynthetic energy storage in a Photosystem II submembrane fraction. The data are treated using an analogy to enzyme kinetics. The semi-reciprocal plot of the energy storage yield (′r) against the modulated measuring beam light intensity (I) provided an energy storage yield extrapolated at i = 0 (′r0), and a half-saturation beam intensity (i50). The energy storage reaction was characterized by a ′r0 of 42% in all the conditions used. However, the valueof i50 was affected by electron-transport inhibitors, artificial electron acceptors, and by the measuring beam wavelength. It was inferred that ′r monitored at any given measuring beam intensity varies with i 50 in close relation to the electron-transport rate monitored as oxygen evolution. We proposed, from the data presented, that the energy storage monitored in photoacoustic experiments is related to the reduction of the plastoquinone pool.

Original languageEnglish (US)
Pages (from-to)370-376
Number of pages7
JournalBBA - Bioenergetics
Volume975
Issue number3
DOIs
StatePublished - Aug 1989
Externally publishedYes

Keywords

  • Electron transfer
  • Energy storage
  • Photoacoustic spectroscopy
  • Photosystem II
  • Plastoquinone

ASJC Scopus subject areas

  • Biophysics

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