Photoacoustic detection of photosynthetic energy storage in Photosystem II submembrane fractions

Robert Carpentier, Roger Leblanc, Murielle Mimeault

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Photoacoustic spectroscopy can be used for the measurement of energy storage during photosynthesis. We report here the monitoring of photosynthetic energy storage in a Photosystem II submembrane fraction. The data are treated using an analogy to enzyme kinetics. The semi-reciprocal plot of the energy storage yield (′r) against the modulated measuring beam light intensity (I) provided an energy storage yield extrapolated at i = 0 (′r0), and a half-saturation beam intensity (i50). The energy storage reaction was characterized by a ′r0 of 42% in all the conditions used. However, the valueof i50 was affected by electron-transport inhibitors, artificial electron acceptors, and by the measuring beam wavelength. It was inferred that ′r monitored at any given measuring beam intensity varies with i 50 in close relation to the electron-transport rate monitored as oxygen evolution. We proposed, from the data presented, that the energy storage monitored in photoacoustic experiments is related to the reduction of the plastoquinone pool.

Original languageEnglish
Pages (from-to)370-376
Number of pages7
JournalBBA - Bioenergetics
Volume975
Issue number3
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Photoacoustic effect
Electron Transport
Energy storage
Plastoquinone
Photosynthesis
Spectrum Analysis
Electrons
Oxygen
Light
Enzymes
Photoacoustic spectroscopy
Enzyme kinetics
Wavelength
Monitoring
Experiments

Keywords

  • Electron transfer
  • Energy storage
  • Photoacoustic spectroscopy
  • Photosystem II
  • Plastoquinone

ASJC Scopus subject areas

  • Biophysics

Cite this

Photoacoustic detection of photosynthetic energy storage in Photosystem II submembrane fractions. / Carpentier, Robert; Leblanc, Roger; Mimeault, Murielle.

In: BBA - Bioenergetics, Vol. 975, No. 3, 01.01.1989, p. 370-376.

Research output: Contribution to journalArticle

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