Photo-crosslinked copolymers of 2-hydroxyethyl methacrylate, poly(ethylene glycol) tetra-acrylate and ethylene dimethacrylate for improving biocompatibility of biosensors

Chris P. Quinn, Chandrashekhar P. Pathak, Adam Heller, Jeffrey A. Hubbell

Research output: Contribution to journalArticle

142 Scopus citations

Abstract

A copolymer containing 88% 2-hydroxyethyl methacrylate (HEMA), 9% poly(ethylene glycol) (MW 18.5 kDa) tetra-acrylate and 3% ethylene dimethacrylate was prepared and evaluated for use as a biocompatible interface between glucose biosensors and tissue in the rat. The glucose sensor utilizes glucose oxidase that is electrically 'wired' to a gold current collector by a reduction-oxidation polymer. Coatings of the copolymer were crosslinked in situ on the sensors using long wavelength ultraviolet light and 2,2-dimethoxy-2-phenyl-acetophenone as the initiator. The effect these films had on the current response to glucose was measured. Over a glucose concentration range of 0-30 mM, the average percentage decrease in response was 45 ± 28% (mean ± 95% confidence interval) at 37°C for films that were about 0.1 mm thick, an acceptable value. Copolymer-treated and control electrodes were implanted in the intrascapular subcutaneous tissue of male Sprague-Dawley rats for three days. The explanted samples were evaluated using scanning electron microscopy. The control electrodes were highly encapsulated with fibrous material, while the copolymer-treated electrodes induced much less encapsulation. The results show this copolymer to be a candidate as a biocompatible coating for electrically wired oxidoreductase-based subcutaneous biosensors.

Original languageEnglish (US)
Pages (from-to)389-396
Number of pages8
JournalBiomaterials
Volume16
Issue number5
DOIs
StatePublished - 1995

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Keywords

  • Biocompatibility
  • Biosensors
  • Glucose oxidase
  • Poly(ethylene glycol)
  • Subcutaneous

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Biophysics
  • Mechanics of Materials
  • Biomaterials
  • Ceramics and Composites

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