Photo-crosslinked copolymers of 2-hydroxyethyl methacrylate, poly(ethylene glycol) tetra-acrylate and ethylene dimethacrylate for improving biocompatibility of biosensors

Chris P. Quinn, Chandrashekhar P. Pathak, Adam Heller, Jeffrey A. Hubbell

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

A copolymer containing 88% 2-hydroxyethyl methacrylate (HEMA), 9% poly(ethylene glycol) (MW 18.5 kDa) tetra-acrylate and 3% ethylene dimethacrylate was prepared and evaluated for use as a biocompatible interface between glucose biosensors and tissue in the rat. The glucose sensor utilizes glucose oxidase that is electrically 'wired' to a gold current collector by a reduction-oxidation polymer. Coatings of the copolymer were crosslinked in situ on the sensors using long wavelength ultraviolet light and 2,2-dimethoxy-2-phenyl-acetophenone as the initiator. The effect these films had on the current response to glucose was measured. Over a glucose concentration range of 0-30 mM, the average percentage decrease in response was 45 ± 28% (mean ± 95% confidence interval) at 37°C for films that were about 0.1 mm thick, an acceptable value. Copolymer-treated and control electrodes were implanted in the intrascapular subcutaneous tissue of male Sprague-Dawley rats for three days. The explanted samples were evaluated using scanning electron microscopy. The control electrodes were highly encapsulated with fibrous material, while the copolymer-treated electrodes induced much less encapsulation. The results show this copolymer to be a candidate as a biocompatible coating for electrically wired oxidoreductase-based subcutaneous biosensors.

Original languageEnglish
Pages (from-to)389-396
Number of pages8
JournalBiomaterials
Volume16
Issue number5
DOIs
StatePublished - Mar 1 1995
Externally publishedYes

Fingerprint

Biosensing Techniques
Biocompatibility
Biosensors
Polyethylene glycols
Ethylene
Copolymers
Glucose
Electrodes
Rats
Glucose Oxidase
Implanted Electrodes
Ethylene Glycol
Tissue
Subcutaneous Tissue
Glucose sensors
Ultraviolet Rays
Coatings
Glucose oxidase
Gold
Electron Scanning Microscopy

Keywords

  • Biocompatibility
  • Biosensors
  • Glucose oxidase
  • Poly(ethylene glycol)
  • Subcutaneous

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Biophysics
  • Mechanics of Materials
  • Biomaterials
  • Ceramics and Composites

Cite this

Photo-crosslinked copolymers of 2-hydroxyethyl methacrylate, poly(ethylene glycol) tetra-acrylate and ethylene dimethacrylate for improving biocompatibility of biosensors. / Quinn, Chris P.; Pathak, Chandrashekhar P.; Heller, Adam; Hubbell, Jeffrey A.

In: Biomaterials, Vol. 16, No. 5, 01.03.1995, p. 389-396.

Research output: Contribution to journalArticle

Quinn, Chris P. ; Pathak, Chandrashekhar P. ; Heller, Adam ; Hubbell, Jeffrey A. / Photo-crosslinked copolymers of 2-hydroxyethyl methacrylate, poly(ethylene glycol) tetra-acrylate and ethylene dimethacrylate for improving biocompatibility of biosensors. In: Biomaterials. 1995 ; Vol. 16, No. 5. pp. 389-396.
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