PHOTO ACOUSTIC SPECTRA OF PHYCOBILIPROTEINS and CHLOROPHYLLIN IN ISOTROPIC and ANISOTROPIC POLYVINYL ALCOHOL FILMS

Danuta Frackowiak, Surat Hotchandani, Roger Leblanc

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Abstract— Photoacoustic spectra (PAS) of biliproteins: R‐phycoerythrin (PE) with R‐phycocyanin (PC) admixture in isotropic and anisotropic polyvinyl‐alcohol (PVA) films were measured. The mixtures of biliproteins and chlorophyllin (Chllin) in PVA were also investigated. In order to establish the influence of PVA anisotropy on PAS amplitude, the stretched and unstretched PVA films colored by Congo red were studied. The films with PE and Chllin mixture when illuminated in the region of PE absorption, show higher PAS signals than the films with PE alone. This is explained by the excitation energy transfer from efficiently fluorescent PE to Chllin that has much lower yield of fluorescence. The stretched films always show a higher PAS signal than isotropic films because of the increase in the thermal conductivity of PVA. But normalized PAS of Congo red and Chllin in stretched PVA are similar to those in unstretched films. Pigment molecules oriented parallel to the direction of film stretching have a lower rate of thermal deactivation compared to that of unoriented molecules as has been found by comparing PAS of differently oriented Congo red molecules using photoselection by polarized light. The same result was obtained for various PE chromophores which are differently oriented in anisotropic films.

Original languageEnglish (US)
Pages (from-to)559-565
Number of pages7
JournalPhotochemistry and Photobiology
Volume42
Issue number5
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Phycobiliproteins
Polyvinyl Alcohol
polyvinyl alcohol
Acoustics
Congo Red
Photoacoustic effect
acoustics
Thermal Conductivity
Energy Transfer
Anisotropy
Molecules
Hot Temperature
Fluorescence
Light
chlorophyllin
molecules
Excitation energy
Light polarization
Chromophores
pigments

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

PHOTO ACOUSTIC SPECTRA OF PHYCOBILIPROTEINS and CHLOROPHYLLIN IN ISOTROPIC and ANISOTROPIC POLYVINYL ALCOHOL FILMS. / Frackowiak, Danuta; Hotchandani, Surat; Leblanc, Roger.

In: Photochemistry and Photobiology, Vol. 42, No. 5, 1985, p. 559-565.

Research output: Contribution to journalArticle

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