Abstract
In rats, administration of 0.3% aluminum in the drinking water for 4-5 weeks significantly increased the in vivo incorporation of 32-phosphorus (3Pi) into proteins with apparent molecular weights of 300 and 210 kDa in the brainstem and cerebral cortex. The identities of these two phosphoproteins as microtubule-associated protein-2 (MAP-2) and the 200 kDa neurofilament subunit (NF), respectively, were established using immunoprecipitation techniques with monoclonal antibodies. Aluminum treatment did not significantly change the amount of MAP-2 or 200 kDa NF in the cerebral cortex and brainstem. Phosphorylation of MAP-2 in aluminum-treated rats in the brainstem and cerebral cortex was 163 and 155% of control values, respectively. The phosphorylation of 200 kDa NF in the brainstem and cerebral cortex of aluminum-treated rats was 148 and 209% of control values, respectively. These results demonstrate that chronic oral aluminum administration to rats increases the phosphorylation of certain cytoskeletal proteins. This treatment regimen may provide a model system with which the mechanisms and consequences of altered in vivo phosphorylation of cytoskeletal proteins can be studied.
Original language | English (US) |
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Pages (from-to) | 95-103 |
Number of pages | 9 |
Journal | Brain Research |
Volume | 456 |
Issue number | 1 |
DOIs | |
State | Published - Jul 19 1988 |
Externally published | Yes |
Keywords
- Aluminum
- Cytoskeletal protein phosphorylation
- Microtubule-associated protein 2
- Neurofilament
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)