Abstract
In rats, administration of 0.3% aluminum in the drinking water for 4-5 weeks significantly increased the in vivo incorporation of 32-phosphorus (3Pi) into proteins with apparent molecular weights of 300 and 210 kDa in the brainstem and cerebral cortex. The identities of these two phosphoproteins as microtubule-associated protein-2 (MAP-2) and the 200 kDa neurofilament subunit (NF), respectively, were established using immunoprecipitation techniques with monoclonal antibodies. Aluminum treatment did not significantly change the amount of MAP-2 or 200 kDa NF in the cerebral cortex and brainstem. Phosphorylation of MAP-2 in aluminum-treated rats in the brainstem and cerebral cortex was 163 and 155% of control values, respectively. The phosphorylation of 200 kDa NF in the brainstem and cerebral cortex of aluminum-treated rats was 148 and 209% of control values, respectively. These results demonstrate that chronic oral aluminum administration to rats increases the phosphorylation of certain cytoskeletal proteins. This treatment regimen may provide a model system with which the mechanisms and consequences of altered in vivo phosphorylation of cytoskeletal proteins can be studied.
| Original language | English |
|---|---|
| Pages (from-to) | 95-103 |
| Number of pages | 9 |
| Journal | Brain Research |
| Volume | 456 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 19 1988 |
| Externally published | Yes |
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Keywords
- Aluminum
- Cytoskeletal protein phosphorylation
- Microtubule-associated protein 2
- Neurofilament
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Phosphorylation of rat brain cytoskeletal proteins is increased after orally administered aluminum. / Johnson, Gail V W; Jope, Richard S.
In: Brain Research, Vol. 456, No. 1, 19.07.1988, p. 95-103.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphorylation of rat brain cytoskeletal proteins is increased after orally administered aluminum
AU - Johnson, Gail V W
AU - Jope, Richard S
PY - 1988/7/19
Y1 - 1988/7/19
N2 - In rats, administration of 0.3% aluminum in the drinking water for 4-5 weeks significantly increased the in vivo incorporation of 32-phosphorus (3Pi) into proteins with apparent molecular weights of 300 and 210 kDa in the brainstem and cerebral cortex. The identities of these two phosphoproteins as microtubule-associated protein-2 (MAP-2) and the 200 kDa neurofilament subunit (NF), respectively, were established using immunoprecipitation techniques with monoclonal antibodies. Aluminum treatment did not significantly change the amount of MAP-2 or 200 kDa NF in the cerebral cortex and brainstem. Phosphorylation of MAP-2 in aluminum-treated rats in the brainstem and cerebral cortex was 163 and 155% of control values, respectively. The phosphorylation of 200 kDa NF in the brainstem and cerebral cortex of aluminum-treated rats was 148 and 209% of control values, respectively. These results demonstrate that chronic oral aluminum administration to rats increases the phosphorylation of certain cytoskeletal proteins. This treatment regimen may provide a model system with which the mechanisms and consequences of altered in vivo phosphorylation of cytoskeletal proteins can be studied.
AB - In rats, administration of 0.3% aluminum in the drinking water for 4-5 weeks significantly increased the in vivo incorporation of 32-phosphorus (3Pi) into proteins with apparent molecular weights of 300 and 210 kDa in the brainstem and cerebral cortex. The identities of these two phosphoproteins as microtubule-associated protein-2 (MAP-2) and the 200 kDa neurofilament subunit (NF), respectively, were established using immunoprecipitation techniques with monoclonal antibodies. Aluminum treatment did not significantly change the amount of MAP-2 or 200 kDa NF in the cerebral cortex and brainstem. Phosphorylation of MAP-2 in aluminum-treated rats in the brainstem and cerebral cortex was 163 and 155% of control values, respectively. The phosphorylation of 200 kDa NF in the brainstem and cerebral cortex of aluminum-treated rats was 148 and 209% of control values, respectively. These results demonstrate that chronic oral aluminum administration to rats increases the phosphorylation of certain cytoskeletal proteins. This treatment regimen may provide a model system with which the mechanisms and consequences of altered in vivo phosphorylation of cytoskeletal proteins can be studied.
KW - Aluminum
KW - Cytoskeletal protein phosphorylation
KW - Microtubule-associated protein 2
KW - Neurofilament
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U2 - 10.1016/0006-8993(88)90350-2
DO - 10.1016/0006-8993(88)90350-2
M3 - Article
VL - 456
SP - 95
EP - 103
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -