Phosphorylation of p53 serine 15 increases interaction with CBP

Paul F. Lambert, Fatah Kashanchi, Michael F. Radonovich, Ramin Shiekhattar, John N. Brady

Research output: Contribution to journalArticle

339 Citations (Scopus)

Abstract

p53 exerts its cell cycle regulatory effects through its ability to function as a sequence-specific DNA binding transcription factor. CREB- binding protein (CBP)/p300, through its interaction with the N terminus of p53, acts as a coactivator for p53 and increases the sequence-specific DNA- binding activity of p53 by acetylating its C terminus. The same N-terminal domain of p53 has recently been shown to be phosphorylated at Ser15 in response to γ-irradiation. Remarkably, we now demonstrate that phosphorylation of p53 at Ser15 increases its ability to recruit CBP/p300. The increase in CBP/p300 binding was followed by an increase in the overall level of acetylation of the C terminus of p53. These results provide a mechanism for the activation of p53-regulated genes following DNA damage, through a signaling pathway linking p53 N-terminal kinase and C-terminal acetyltransferase activities.

Original languageEnglish (US)
Pages (from-to)33048-33053
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number49
DOIs
StatePublished - Dec 4 1998
Externally publishedYes

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CREB-Binding Protein
Phosphorylation
Serine
DNA
Acetylation
Acetyltransferases
p53 Genes
Protein Binding
DNA Damage
Cell Cycle
Transcription Factors
Phosphotransferases
Genes
Chemical activation
Cells
Irradiation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Phosphorylation of p53 serine 15 increases interaction with CBP. / Lambert, Paul F.; Kashanchi, Fatah; Radonovich, Michael F.; Shiekhattar, Ramin; Brady, John N.

In: Journal of Biological Chemistry, Vol. 273, No. 49, 04.12.1998, p. 33048-33053.

Research output: Contribution to journalArticle

Lambert, PF, Kashanchi, F, Radonovich, MF, Shiekhattar, R & Brady, JN 1998, 'Phosphorylation of p53 serine 15 increases interaction with CBP', Journal of Biological Chemistry, vol. 273, no. 49, pp. 33048-33053. https://doi.org/10.1074/jbc.273.49.33048
Lambert, Paul F. ; Kashanchi, Fatah ; Radonovich, Michael F. ; Shiekhattar, Ramin ; Brady, John N. / Phosphorylation of p53 serine 15 increases interaction with CBP. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 49. pp. 33048-33053.
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