Phosphorylation-dependent inhibition of Cdc42 GEF Gef1 by 14-3-3 protein Rad24 spatially regulates Cdc42 GTPase activity and oscillatory dynamics during cell morphogenesis

Maitreyi Das, Illyce Nuñeza, Marbelys Rodriguez, David J. Wiley, Juan Rodriguez, Ali Sarkeshik, John R. Yates, Peter Buchwald, Fulvia Verde

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Active Cdc42 GTPase, a key regulator of cell polarity, displays oscillatory dynamics that are anticorrelated at the two cell tips in fission yeast. Anticorrelation suggests competition for active Cdc42 or for its effectors. Here we show how 14-3-3 protein Rad24 associates with Cdc42 guanine exchange factor (GEF) Gef1, limiting Gef1 availability to promote Cdc42 activation. Phosphorylation of Gef1 by conserved NDR kinase Orb6 promotes Gef1 binding to Rad24. Loss of Rad24-Gef1 interaction increases Gef1 protein localization and Cdc42 activation at the cell tips and reduces the anticorrelation of active Cdc42 oscillations. Increased Cdc42 activation promotes precocious bipolar growth activation, bypassing the normal requirement for an intact microtubule cytoskeleton and for microtubule-dependent polarity landmark Tea4-PP1. Further, increased Cdc42 activation by Gef1 widens cell diameter and alters tip curvature, countering the effects of Cdc42 GTPase-activating protein Rga4. The respective levels of Gef1 and Rga4 proteins at the membrane define dynamically the growing area at each cell tip. Our findings show how the 14-3-3 protein Rad24 modulates the availability of Cdc42 GEF Gef1, a homologue of mammalian Cdc42 GEF DNMBP/TUBA, to spatially control Cdc42 GTPase activity and promote cell polarization and cell shape emergence.

Original languageEnglish (US)
Pages (from-to)3520-3534
Number of pages15
JournalMolecular Biology of the Cell
Volume26
Issue number19
DOIs
StatePublished - Oct 1 2015

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14-3-3 Proteins
GTP Phosphohydrolases
Guanine
Morphogenesis
Phosphorylation
cdc42 GTP-Binding Protein
Microtubules
GTPase-Activating Proteins
Cell Polarity
Cell Shape
Schizosaccharomyces
Cytoskeleton
Membrane Proteins
Phosphotransferases
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Phosphorylation-dependent inhibition of Cdc42 GEF Gef1 by 14-3-3 protein Rad24 spatially regulates Cdc42 GTPase activity and oscillatory dynamics during cell morphogenesis. / Das, Maitreyi; Nuñeza, Illyce; Rodriguez, Marbelys; Wiley, David J.; Rodriguez, Juan; Sarkeshik, Ali; Yates, John R.; Buchwald, Peter; Verde, Fulvia.

In: Molecular Biology of the Cell, Vol. 26, No. 19, 01.10.2015, p. 3520-3534.

Research output: Contribution to journalArticle

Das, Maitreyi ; Nuñeza, Illyce ; Rodriguez, Marbelys ; Wiley, David J. ; Rodriguez, Juan ; Sarkeshik, Ali ; Yates, John R. ; Buchwald, Peter ; Verde, Fulvia. / Phosphorylation-dependent inhibition of Cdc42 GEF Gef1 by 14-3-3 protein Rad24 spatially regulates Cdc42 GTPase activity and oscillatory dynamics during cell morphogenesis. In: Molecular Biology of the Cell. 2015 ; Vol. 26, No. 19. pp. 3520-3534.
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