Phosphorous metabolite and cell cycle kinetic response of two human squamous cell carcinomas to radiation

Srinivasan Vijayakumar, Lech Czerski, Anthony Majors, Rafael Valenzuela, Mike Beckett, Ralph Weichselbaum, Thian Ng

Research output: Contribution to journalArticle

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Abstract

Phosphorous metabolism and cell cycle phase kinetics in response to radiation of two perfused human squamous cell carcinoma cell lines, SQ20B (radioresistant) and SQ38 (relatively radiosensitive), embedded in both basement membrane (Matrigel) and agarose gel threads were studied. The findings for these human cancer cells in response to 2- and 50-Gy irradiation are as follows. (a) Well perfused pure cancer cells (both SQ20B and SQ38) in both proliferate (cells embedded in Matrigel) and static (cells embedded in agarose threads) states did not show significant alteration in either phosphorous bioenergetics or membrane metabolites at 24 and 48 h after irradiation, although a large fraction of the population was clonogenically impaired. Previously reported, sensitively detected, metabolite alterations in response to radiation in rodent and human tumors in situ were not seen in these homogeneous cancer cell populations. (b) The radiosensitive squamous cell carcinoma cell line SQ38 exhibited G1 block (from 54.38 ± 1.40% in control to 73.93 ± 1.01% after irradiation; mean ± SD) in response to low-dose 2-Gy irradiation and G2 block (from 12.98 ± 2.15% in control to 25.6 ± 3.15% after irradiation) in response to high-dose 50-Gy irradiation, while the radioresistant cell line SQ20B showed only conventional G2 block in response to both doses. The differential cell cycle phase response may indicate the difference in radioresistance. (c) The membrane metabolites (including phosphomonoesters and phosphodiesters) and phosphocreatine gradually increased from the early passages to late passages, suggesting that cell proliferation rates were increasing as the cells adapted to tissue culture. The results suggest that the radiation-induced metabolite changes observed in solid tumors in situ may not be a direct response to interim changes within the cancer cells but, rather, a consequence of radiation damage either to the vasculature or to other host-mediated factors.

Original languageEnglish
Pages (from-to)5299-5306
Number of pages8
JournalCancer Research
Volume52
Issue number19
StatePublished - Oct 1 1992
Externally publishedYes

Fingerprint

Squamous Cell Carcinoma
Cell Cycle
Radiation
Neoplasms
Cell Line
Sepharose
Membranes
Phosphocreatine
Basement Membrane
Energy Metabolism
Population
Rodentia
Gels
Cell Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Vijayakumar, S., Czerski, L., Majors, A., Valenzuela, R., Beckett, M., Weichselbaum, R., & Ng, T. (1992). Phosphorous metabolite and cell cycle kinetic response of two human squamous cell carcinomas to radiation. Cancer Research, 52(19), 5299-5306.

Phosphorous metabolite and cell cycle kinetic response of two human squamous cell carcinomas to radiation. / Vijayakumar, Srinivasan; Czerski, Lech; Majors, Anthony; Valenzuela, Rafael; Beckett, Mike; Weichselbaum, Ralph; Ng, Thian.

In: Cancer Research, Vol. 52, No. 19, 01.10.1992, p. 5299-5306.

Research output: Contribution to journalArticle

Vijayakumar, S, Czerski, L, Majors, A, Valenzuela, R, Beckett, M, Weichselbaum, R & Ng, T 1992, 'Phosphorous metabolite and cell cycle kinetic response of two human squamous cell carcinomas to radiation', Cancer Research, vol. 52, no. 19, pp. 5299-5306.
Vijayakumar S, Czerski L, Majors A, Valenzuela R, Beckett M, Weichselbaum R et al. Phosphorous metabolite and cell cycle kinetic response of two human squamous cell carcinomas to radiation. Cancer Research. 1992 Oct 1;52(19):5299-5306.
Vijayakumar, Srinivasan ; Czerski, Lech ; Majors, Anthony ; Valenzuela, Rafael ; Beckett, Mike ; Weichselbaum, Ralph ; Ng, Thian. / Phosphorous metabolite and cell cycle kinetic response of two human squamous cell carcinomas to radiation. In: Cancer Research. 1992 ; Vol. 52, No. 19. pp. 5299-5306.
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abstract = "Phosphorous metabolism and cell cycle phase kinetics in response to radiation of two perfused human squamous cell carcinoma cell lines, SQ20B (radioresistant) and SQ38 (relatively radiosensitive), embedded in both basement membrane (Matrigel) and agarose gel threads were studied. The findings for these human cancer cells in response to 2- and 50-Gy irradiation are as follows. (a) Well perfused pure cancer cells (both SQ20B and SQ38) in both proliferate (cells embedded in Matrigel) and static (cells embedded in agarose threads) states did not show significant alteration in either phosphorous bioenergetics or membrane metabolites at 24 and 48 h after irradiation, although a large fraction of the population was clonogenically impaired. Previously reported, sensitively detected, metabolite alterations in response to radiation in rodent and human tumors in situ were not seen in these homogeneous cancer cell populations. (b) The radiosensitive squamous cell carcinoma cell line SQ38 exhibited G1 block (from 54.38 ± 1.40{\%} in control to 73.93 ± 1.01{\%} after irradiation; mean ± SD) in response to low-dose 2-Gy irradiation and G2 block (from 12.98 ± 2.15{\%} in control to 25.6 ± 3.15{\%} after irradiation) in response to high-dose 50-Gy irradiation, while the radioresistant cell line SQ20B showed only conventional G2 block in response to both doses. The differential cell cycle phase response may indicate the difference in radioresistance. (c) The membrane metabolites (including phosphomonoesters and phosphodiesters) and phosphocreatine gradually increased from the early passages to late passages, suggesting that cell proliferation rates were increasing as the cells adapted to tissue culture. The results suggest that the radiation-induced metabolite changes observed in solid tumors in situ may not be a direct response to interim changes within the cancer cells but, rather, a consequence of radiation damage either to the vasculature or to other host-mediated factors.",
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