Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy

Maria Fernanda Suarez, Maria Carmen Piqueras, Leandro Correa, Evangelina Esposito, Maria Fernanda Barros, Sanjoy K Bhattacharya, Julio A. Urrets-Zavalia, Horacio M. Serra

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g., contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress.

Original languageEnglish (US)
JournalJournal of Cellular Biochemistry
DOIs
StateAccepted/In press - 2017

Fingerprint

Cornea
Phospholipids
Lipids
Phosphatidylserines
Phosphatidylcholines
Lipid Peroxidation
Oxidative Stress
Corneal Opacity
Oxidative stress
Tandem Mass Spectrometry
Climate
Cataract
Pathology
Opacity
Mass spectrometers
Surgery
Mass spectrometry
Proteins
Chemical analysis

Keywords

  • CDK
  • CORNEA
  • PHOSPHATIDYLCHOLINE
  • PHOSPHATIDYLSERINE
  • PHOSPHOLIPIDS
  • TANDEM MASS SPECTROMETRY

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Suarez, M. F., Piqueras, M. C., Correa, L., Esposito, E., Barros, M. F., Bhattacharya, S. K., ... Serra, H. M. (Accepted/In press). Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy. Journal of Cellular Biochemistry. https://doi.org/10.1002/jcb.26045

Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy. / Suarez, Maria Fernanda; Piqueras, Maria Carmen; Correa, Leandro; Esposito, Evangelina; Barros, Maria Fernanda; Bhattacharya, Sanjoy K; Urrets-Zavalia, Julio A.; Serra, Horacio M.

In: Journal of Cellular Biochemistry, 2017.

Research output: Contribution to journalArticle

Suarez, MF, Piqueras, MC, Correa, L, Esposito, E, Barros, MF, Bhattacharya, SK, Urrets-Zavalia, JA & Serra, HM 2017, 'Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy', Journal of Cellular Biochemistry. https://doi.org/10.1002/jcb.26045
Suarez, Maria Fernanda ; Piqueras, Maria Carmen ; Correa, Leandro ; Esposito, Evangelina ; Barros, Maria Fernanda ; Bhattacharya, Sanjoy K ; Urrets-Zavalia, Julio A. ; Serra, Horacio M. / Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy. In: Journal of Cellular Biochemistry. 2017.
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