Phospholipid profiles of control and glaucomatous human aqueous humor

Genea Edwards, Katyayini Aribindi, Yenifer Guerra, Richard K Lee, Sanjoy K Bhattacharya

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To compare phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and phosphatidylinositol) profiles of human control and glaucomatous aqueous humor (AQH). AQH samples were procured during surgery from human POAG and control subjects (n = 15 each). Samples were used following institutional review board approved protocols and adhering to the tenets of the Declaration of Helsinki. Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford's method, and select samples were confirmed with Densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ Quantum Access Max triple quadrupole mass spectrometer utilizing precursor ion scan (PIS) or neutral ion loss scan (NLS) using appropriate class specific lipid standards in a two step quantification process. The comparative profiles of phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines and phosphatidylinositols between control and glaucomatous AQH showed several species common between them. A number of unique lipids in all four phospholipid classes were also identified in control eyes that were absent in glaucomatous eyes and vice versa. A number of phospholipids were found to be uniquely present in control, but absent in glaucomatous AQH and vice versa. Compared with a previous study of control and POAG red blood cells, a number of these phospholipids are absent locally (AQH).

Original languageEnglish
Pages (from-to)232-247
Number of pages16
JournalBiochimie
Volume101
Issue number1
DOIs
StatePublished - Jan 1 2014

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Aqueous Humor
Phospholipids
Phosphatidylserines
Phosphatidylinositols
Phosphatidylcholines
Lipids
Helsinki Declaration
Phosphatidylethanolamines
Ions
Blood Cell Count
Densitometry
Research Ethics Committees
Mass spectrometers
Surgery
Erythrocytes
Gels
Blood
Cells
Proteins
phosphatidylethanolamine

Keywords

  • Aqueous humor
  • Glaucoma
  • Lipidomics
  • Mass spectrometry
  • Phospholipids

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

Phospholipid profiles of control and glaucomatous human aqueous humor. / Edwards, Genea; Aribindi, Katyayini; Guerra, Yenifer; Lee, Richard K; Bhattacharya, Sanjoy K.

In: Biochimie, Vol. 101, No. 1, 01.01.2014, p. 232-247.

Research output: Contribution to journalArticle

Edwards, Genea ; Aribindi, Katyayini ; Guerra, Yenifer ; Lee, Richard K ; Bhattacharya, Sanjoy K. / Phospholipid profiles of control and glaucomatous human aqueous humor. In: Biochimie. 2014 ; Vol. 101, No. 1. pp. 232-247.
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