Phosphoinositide breakdown in blowfly salivary glands.

I. Litosch, H. S. Lee, J. N. Fain

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In blowfly salivary glands, 5-hydroxytryptamine stimulated a rapid and sustained loss of [3H]inositol, [32P]phosphatidylinositol, phosphatidylinositol 4-phosphate, and phosphatidylinositol 4,5-bisphosphate. There was a corresponding increase in labeled inositol phosphates. In the absence of Ca2+, 5-hydroxytryptamine stimulated a rapid but transient loss of labeled phosphatidylinositol 4,5-bisphosphate. By 5 min, the amount of labeled phosphatidylinositol 4,5-bisphosphate recovered to control values. The divalent ionophore A23187 stimulated loss of labeled phosphatidylinositol 4,5-bisphosphate and increased the amount of labeled phosphatidylinositol. In homogenates, Ca2+ stimulated phosphatidylinositol 4,5-bisphosphate breakdown but not phosphatidylinositol breakdown. These results suggest that hormone-stimulated breakdown of labeled phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate occurs through a phospholipase C and is relatively independent of extracellular Ca2+. There is also a Ca2+-activated conversion of phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol.

Original languageEnglish
JournalThe American journal of physiology
Volume246
Issue number1 Pt 1
StatePublished - Jan 1 1984

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Phosphatidylinositols
Salivary Glands
Serotonin
Inositol Phosphates
Ionophores
Calcimycin
Type C Phospholipases
Inositol
Hormones

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Litosch, I., Lee, H. S., & Fain, J. N. (1984). Phosphoinositide breakdown in blowfly salivary glands. The American journal of physiology, 246(1 Pt 1).

Phosphoinositide breakdown in blowfly salivary glands. / Litosch, I.; Lee, H. S.; Fain, J. N.

In: The American journal of physiology, Vol. 246, No. 1 Pt 1, 01.01.1984.

Research output: Contribution to journalArticle

Litosch, I, Lee, HS & Fain, JN 1984, 'Phosphoinositide breakdown in blowfly salivary glands.', The American journal of physiology, vol. 246, no. 1 Pt 1.
Litosch, I. ; Lee, H. S. ; Fain, J. N. / Phosphoinositide breakdown in blowfly salivary glands. In: The American journal of physiology. 1984 ; Vol. 246, No. 1 Pt 1.
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