Phosphocitrate blocks nitric oxide-induced calcification of cartilage and chondrocyte-derived apoptotic bodies

Herman S. Cheung, L. M. Ryan

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Objective: To examine whether phosphocitrate (PC) will block nitric oxide-induced calcification of cartilage or chondrocyte-derived apoptotic bodies. Design: Articular cartilage vesicles (ACV) or apoptotic bodies (AB) were isolated from untreated or 1 mM sodium nitroprusside (SNP) treated porcine cartilage slices. Mineralization of ACV, AB, control untreated and SNP-treated cartilage were done in the presence or absence of PC (1 mM) ± ATP (1 mM). Results: PC [1 mM] blocked both the ATP-dependent and - independent mineralization in ACV and AB, untreated and SNP treated cartilage. Moreover, PC had no effect on NTPPPH activity in either ACV or AB fraction in the presence or absence of ATP suggesting that PC did not block the mineralization through the inhibition of NTPPPH activity. Conclusions: PC inhibits nitric oxide-induced calcification of cartilage and cartilage- derived apoptotic bodies.

Original languageEnglish (US)
Pages (from-to)409-412
Number of pages4
JournalOsteoarthritis and Cartilage
Volume7
Issue number4
DOIs
StatePublished - Jul 1999

Keywords

  • Cartilage
  • Mineralization
  • Nitric oxide
  • Phosphocitrate

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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