Phleboviruses associated with sand flies in arid bio-geographical areas of Central Tunisia

K. Dachraoui, W. Fares, L. Bichaud, W. Barhoumi, John C Beier, M. Derbali, S. Cherni, X. de Lamballerie, I. Chelbi, R. N. Charrel, E. Zhioua

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

An entomological investigation was carried out in 2014 at two sites located in Central Tunisia, one irrigated and another non-irrigated situated in arid bio-geographical areas. Sand flies of the subgenus Larroussius namely Phlebotomus perfiliewi, Phlebotomus perniciosus, and Phlebotomus longicuspis are the most abundant sand fly species in the irrigated site. However, in the non-irrigated site, Phlebotomus papatasi of the Phlebotomus genus is the most abundant species. A total of 3191 sand flies were collected and pooled with up to 30 specimens per pool based on sex, trapping location and collection date, were tested for the presence of phleboviruses by nested reverse transcriptase polymerase chain reaction in the polymerase gene and sequenced. Of a total of 117 pools, 4 were positive, yielding a minimum infection rate of sand flies with phleboviruses of 0.12%. Phylogenetic analysis performed using partial nucleotide and amino acid sequence in the polymerase gene showed that these phleboviruses belonged to four different clusters corresponding to Toscana virus (TOSV), Saddaguia virus (SADV), Sandfly Fever Sicilian Virus (SFSV) and Utique virus (UTIV). This study provides more evidence that the abundance of P. perfiliewi is associated with the development of irrigation in arid bio-geographical areas of Central Tunisia which may have led to the emergence of phleboviruses. We report the first detection of TOSV from sand flies collected from Central Tunisia.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalActa Tropica
Volume158
DOIs
StatePublished - Jun 1 2016

Fingerprint

Phlebovirus
Phlebotomus
Psychodidae
Tunisia
Phlebotominae
Sandfly fever Naples virus
Phlebotomus perniciosus
Phlebotomus papatasi
Viruses
viruses
irrigated conditions
Reverse Transcriptase Polymerase Chain Reaction
Genes
trapping
Amino Acid Sequence
genes
amino acid sequences
Nucleotides
nucleotides
reverse transcriptase polymerase chain reaction

Keywords

  • Emerging
  • Mediterranean basin
  • Phlebotomus
  • Phlebovirus
  • Saddaguia virus
  • Sand flies
  • Sandfly fever sicilian virus
  • Toscana virus
  • Utique virus

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Phleboviruses associated with sand flies in arid bio-geographical areas of Central Tunisia. / Dachraoui, K.; Fares, W.; Bichaud, L.; Barhoumi, W.; Beier, John C; Derbali, M.; Cherni, S.; Lamballerie, X. de; Chelbi, I.; Charrel, R. N.; Zhioua, E.

In: Acta Tropica, Vol. 158, 01.06.2016, p. 13-19.

Research output: Contribution to journalArticle

Dachraoui, K, Fares, W, Bichaud, L, Barhoumi, W, Beier, JC, Derbali, M, Cherni, S, Lamballerie, XD, Chelbi, I, Charrel, RN & Zhioua, E 2016, 'Phleboviruses associated with sand flies in arid bio-geographical areas of Central Tunisia', Acta Tropica, vol. 158, pp. 13-19. https://doi.org/10.1016/j.actatropica.2016.02.008
Dachraoui, K. ; Fares, W. ; Bichaud, L. ; Barhoumi, W. ; Beier, John C ; Derbali, M. ; Cherni, S. ; Lamballerie, X. de ; Chelbi, I. ; Charrel, R. N. ; Zhioua, E. / Phleboviruses associated with sand flies in arid bio-geographical areas of Central Tunisia. In: Acta Tropica. 2016 ; Vol. 158. pp. 13-19.
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AU - Fares, W.

AU - Bichaud, L.

AU - Barhoumi, W.

AU - Beier, John C

AU - Derbali, M.

AU - Cherni, S.

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AU - Charrel, R. N.

AU - Zhioua, E.

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