Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration

Daniel J. Cooper, Giulia Zunino, John L. Bixby, Vance P. Lemmon

Research output: Contribution to journalReview article

6 Scopus citations

Abstract

High-throughput, target-based screening techniques have been utilized extensively for drug discovery in the past several decades. However, the need for more predictive in vitro models of in vivo disease states has generated a shift in strategy towards phenotype-based screens. Phenotype based screens are particularly valuable in studying complex conditions such as CNS injury and degenerative disease, as many factors can contribute to a specific cellular response. In this review, we will discuss different screening frameworks and their relative utility in examining mechanisms of neurodegeneration and axon regrowth, particularly in cell-based in vitro disease models.

Original languageEnglish (US)
Pages (from-to)161-169
Number of pages9
JournalMolecular and Cellular Neuroscience
Volume80
DOIs
StatePublished - Apr 1 2017

Keywords

  • Axon degeneration
  • Axon growth
  • Axon regeneration
  • High content analysis
  • High throughput screening
  • Phenotypic analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Fingerprint Dive into the research topics of 'Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration'. Together they form a unique fingerprint.

  • Cite this