Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration

Daniel J. Cooper, Giulia Zunino, John Bixby, Vance Lemmon

Research output: Contribution to journalArticle

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Abstract

High-throughput, target-based screening techniques have been utilized extensively for drug discovery in the past several decades. However, the need for more predictive . in vitro models of . in vivo disease states has generated a shift in strategy towards phenotype-based screens. Phenotype based screens are particularly valuable in studying complex conditions such as CNS injury and degenerative disease, as many factors can contribute to a specific cellular response. In this review, we will discuss different screening frameworks and their relative utility in examining mechanisms of neurodegeneration and axon regrowth, particularly in cell-based . in vitro disease models.

Original languageEnglish (US)
JournalMolecular and Cellular Neuroscience
DOIs
StateAccepted/In press - Apr 25 2016

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Keywords

  • Axon degeneration
  • Axon growth
  • Axon regeneration
  • High content analysis
  • High throughput screening
  • Phenotypic analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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