Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration

Daniel J. Cooper, Giulia Zunino, John Bixby, Vance Lemmon

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

High-throughput, target-based screening techniques have been utilized extensively for drug discovery in the past several decades. However, the need for more predictive . in vitro models of . in vivo disease states has generated a shift in strategy towards phenotype-based screens. Phenotype based screens are particularly valuable in studying complex conditions such as CNS injury and degenerative disease, as many factors can contribute to a specific cellular response. In this review, we will discuss different screening frameworks and their relative utility in examining mechanisms of neurodegeneration and axon regrowth, particularly in cell-based . in vitro disease models.

Original languageEnglish (US)
JournalMolecular and Cellular Neuroscience
DOIs
StateAccepted/In press - Apr 25 2016

Fingerprint

Regeneration
Neurons
Pharmaceutical Preparations
Phenotype
Drug Discovery
Axons
Wounds and Injuries
In Vitro Techniques

Keywords

  • Axon degeneration
  • Axon growth
  • Axon regeneration
  • High content analysis
  • High throughput screening
  • Phenotypic analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration. / Cooper, Daniel J.; Zunino, Giulia; Bixby, John; Lemmon, Vance.

In: Molecular and Cellular Neuroscience, 25.04.2016.

Research output: Contribution to journal › Article

Cooper, DJ, Zunino, G, Bixby, J & Lemmon, V 2016, 'Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration', Molecular and Cellular Neuroscience. https://doi.org/10.1016/j.mcn.2016.07.001
Cooper DJ, Zunino G, Bixby J, Lemmon V. Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration. Molecular and Cellular Neuroscience. 2016 Apr 25. https://doi.org/10.1016/j.mcn.2016.07.001
Cooper, Daniel J. ; Zunino, Giulia ; Bixby, John ; Lemmon, Vance. / Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration. In: Molecular and Cellular Neuroscience. 2016.
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