Abstract
In this study, we carried out a phenotypic and functional characterization of lymphocytes isolated from the uterine endometrium of the pregnant rhesus monkey. A majority (80%) of these cells were CD56bright+, CD3- had typical large granular lymphocyte/uterine natural killer (NK) cell morphology and contained numerous cytoplasmic granules. Flow cytometric evaluation showed that rhesus decidual CD56bright+ cells shared other phenotypic features of human uterine NK cells, including low levels of CD45RA and CD62L expression. A majority of the rhesus uterine CD56bright+ cells expressed low levels of CD 16 but were CD2-. In contrast, most rhesus CD16+ peripheral blood cells were CD56-. In addition to the primary population of CD56bright+ cells, a minor subset of smaller and less granular CD56intermediate+ decidual lymphocytes was identified, the majority of which were CD16-, CD2+. Decidual CD56+ cells did not express monocyte/macrophage markers, including CD14, CD64 and CD68. Decidual lymphocytes effectively lysed K562, Raji and particularly 721.221 targets in cytotoxicity assays. Together, these results suggest that as in human pregnancy, rhesus decidual CD56bright+ cells represent a distinct lymphocyte subset that belongs to the NK cell lineage.
| Original language | English |
|---|---|
| Pages (from-to) | 57-79 |
| Number of pages | 23 |
| Journal | Journal of Reproductive Immunology |
| Volume | 50 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 17 2001 |
| Externally published | Yes |
Fingerprint
Keywords
- Decidua
- HLA-G
- Major histocompatibility complex
- NK cell
- Placenta
- Rhesus monkey
ASJC Scopus subject areas
- Immunology
- Reproductive Medicine
Cite this
Phenotypic and functional characterization of rhesus monkey decidual lymphocytes : Rhesus decidual large granular lymphocytes express CD56 and have cytolytic activity. / Slukvin, I. I.; Watkins, David; Golos, T. G.
In: Journal of Reproductive Immunology, Vol. 50, No. 1, 17.03.2001, p. 57-79.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phenotypic and functional characterization of rhesus monkey decidual lymphocytes
T2 - Rhesus decidual large granular lymphocytes express CD56 and have cytolytic activity
AU - Slukvin, I. I.
AU - Watkins, David
AU - Golos, T. G.
PY - 2001/3/17
Y1 - 2001/3/17
N2 - In this study, we carried out a phenotypic and functional characterization of lymphocytes isolated from the uterine endometrium of the pregnant rhesus monkey. A majority (80%) of these cells were CD56bright+, CD3- had typical large granular lymphocyte/uterine natural killer (NK) cell morphology and contained numerous cytoplasmic granules. Flow cytometric evaluation showed that rhesus decidual CD56bright+ cells shared other phenotypic features of human uterine NK cells, including low levels of CD45RA and CD62L expression. A majority of the rhesus uterine CD56bright+ cells expressed low levels of CD 16 but were CD2-. In contrast, most rhesus CD16+ peripheral blood cells were CD56-. In addition to the primary population of CD56bright+ cells, a minor subset of smaller and less granular CD56intermediate+ decidual lymphocytes was identified, the majority of which were CD16-, CD2+. Decidual CD56+ cells did not express monocyte/macrophage markers, including CD14, CD64 and CD68. Decidual lymphocytes effectively lysed K562, Raji and particularly 721.221 targets in cytotoxicity assays. Together, these results suggest that as in human pregnancy, rhesus decidual CD56bright+ cells represent a distinct lymphocyte subset that belongs to the NK cell lineage.
AB - In this study, we carried out a phenotypic and functional characterization of lymphocytes isolated from the uterine endometrium of the pregnant rhesus monkey. A majority (80%) of these cells were CD56bright+, CD3- had typical large granular lymphocyte/uterine natural killer (NK) cell morphology and contained numerous cytoplasmic granules. Flow cytometric evaluation showed that rhesus decidual CD56bright+ cells shared other phenotypic features of human uterine NK cells, including low levels of CD45RA and CD62L expression. A majority of the rhesus uterine CD56bright+ cells expressed low levels of CD 16 but were CD2-. In contrast, most rhesus CD16+ peripheral blood cells were CD56-. In addition to the primary population of CD56bright+ cells, a minor subset of smaller and less granular CD56intermediate+ decidual lymphocytes was identified, the majority of which were CD16-, CD2+. Decidual CD56+ cells did not express monocyte/macrophage markers, including CD14, CD64 and CD68. Decidual lymphocytes effectively lysed K562, Raji and particularly 721.221 targets in cytotoxicity assays. Together, these results suggest that as in human pregnancy, rhesus decidual CD56bright+ cells represent a distinct lymphocyte subset that belongs to the NK cell lineage.
KW - Decidua
KW - HLA-G
KW - Major histocompatibility complex
KW - NK cell
KW - Placenta
KW - Rhesus monkey
UR - http://www.scopus.com/inward/record.url?scp=0035112039&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035112039&partnerID=8YFLogxK
U2 - 10.1016/S0165-0378(00)00090-5
DO - 10.1016/S0165-0378(00)00090-5
M3 - Article
C2 - 11254941
AN - SCOPUS:0035112039
VL - 50
SP - 57
EP - 79
JO - Journal of Reproductive Immunology
JF - Journal of Reproductive Immunology
SN - 0165-0378
IS - 1
ER -