Phenotypic and functional characterization of rhesus monkey decidual lymphocytes: Rhesus decidual large granular lymphocytes express CD56 and have cytolytic activity

I. I. Slukvin, David Watkins, T. G. Golos

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In this study, we carried out a phenotypic and functional characterization of lymphocytes isolated from the uterine endometrium of the pregnant rhesus monkey. A majority (80%) of these cells were CD56bright+, CD3- had typical large granular lymphocyte/uterine natural killer (NK) cell morphology and contained numerous cytoplasmic granules. Flow cytometric evaluation showed that rhesus decidual CD56bright+ cells shared other phenotypic features of human uterine NK cells, including low levels of CD45RA and CD62L expression. A majority of the rhesus uterine CD56bright+ cells expressed low levels of CD 16 but were CD2-. In contrast, most rhesus CD16+ peripheral blood cells were CD56-. In addition to the primary population of CD56bright+ cells, a minor subset of smaller and less granular CD56intermediate+ decidual lymphocytes was identified, the majority of which were CD16-, CD2+. Decidual CD56+ cells did not express monocyte/macrophage markers, including CD14, CD64 and CD68. Decidual lymphocytes effectively lysed K562, Raji and particularly 721.221 targets in cytotoxicity assays. Together, these results suggest that as in human pregnancy, rhesus decidual CD56bright+ cells represent a distinct lymphocyte subset that belongs to the NK cell lineage.

Original languageEnglish
Pages (from-to)57-79
Number of pages23
JournalJournal of Reproductive Immunology
Volume50
Issue number1
DOIs
StatePublished - Mar 17 2001
Externally publishedYes

Fingerprint

Macaca mulatta
Lymphocytes
Natural Killer Cells
Cytoplasmic Granules
Lymphocyte Subsets
Cell Lineage
Endometrium
Monocytes
Blood Cells
Macrophages
Pregnancy
Population

Keywords

  • Decidua
  • HLA-G
  • Major histocompatibility complex
  • NK cell
  • Placenta
  • Rhesus monkey

ASJC Scopus subject areas

  • Immunology
  • Reproductive Medicine

Cite this

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title = "Phenotypic and functional characterization of rhesus monkey decidual lymphocytes: Rhesus decidual large granular lymphocytes express CD56 and have cytolytic activity",
abstract = "In this study, we carried out a phenotypic and functional characterization of lymphocytes isolated from the uterine endometrium of the pregnant rhesus monkey. A majority (80{\%}) of these cells were CD56bright+, CD3- had typical large granular lymphocyte/uterine natural killer (NK) cell morphology and contained numerous cytoplasmic granules. Flow cytometric evaluation showed that rhesus decidual CD56bright+ cells shared other phenotypic features of human uterine NK cells, including low levels of CD45RA and CD62L expression. A majority of the rhesus uterine CD56bright+ cells expressed low levels of CD 16 but were CD2-. In contrast, most rhesus CD16+ peripheral blood cells were CD56-. In addition to the primary population of CD56bright+ cells, a minor subset of smaller and less granular CD56intermediate+ decidual lymphocytes was identified, the majority of which were CD16-, CD2+. Decidual CD56+ cells did not express monocyte/macrophage markers, including CD14, CD64 and CD68. Decidual lymphocytes effectively lysed K562, Raji and particularly 721.221 targets in cytotoxicity assays. Together, these results suggest that as in human pregnancy, rhesus decidual CD56bright+ cells represent a distinct lymphocyte subset that belongs to the NK cell lineage.",
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T2 - Rhesus decidual large granular lymphocytes express CD56 and have cytolytic activity

AU - Slukvin, I. I.

AU - Watkins, David

AU - Golos, T. G.

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N2 - In this study, we carried out a phenotypic and functional characterization of lymphocytes isolated from the uterine endometrium of the pregnant rhesus monkey. A majority (80%) of these cells were CD56bright+, CD3- had typical large granular lymphocyte/uterine natural killer (NK) cell morphology and contained numerous cytoplasmic granules. Flow cytometric evaluation showed that rhesus decidual CD56bright+ cells shared other phenotypic features of human uterine NK cells, including low levels of CD45RA and CD62L expression. A majority of the rhesus uterine CD56bright+ cells expressed low levels of CD 16 but were CD2-. In contrast, most rhesus CD16+ peripheral blood cells were CD56-. In addition to the primary population of CD56bright+ cells, a minor subset of smaller and less granular CD56intermediate+ decidual lymphocytes was identified, the majority of which were CD16-, CD2+. Decidual CD56+ cells did not express monocyte/macrophage markers, including CD14, CD64 and CD68. Decidual lymphocytes effectively lysed K562, Raji and particularly 721.221 targets in cytotoxicity assays. Together, these results suggest that as in human pregnancy, rhesus decidual CD56bright+ cells represent a distinct lymphocyte subset that belongs to the NK cell lineage.

AB - In this study, we carried out a phenotypic and functional characterization of lymphocytes isolated from the uterine endometrium of the pregnant rhesus monkey. A majority (80%) of these cells were CD56bright+, CD3- had typical large granular lymphocyte/uterine natural killer (NK) cell morphology and contained numerous cytoplasmic granules. Flow cytometric evaluation showed that rhesus decidual CD56bright+ cells shared other phenotypic features of human uterine NK cells, including low levels of CD45RA and CD62L expression. A majority of the rhesus uterine CD56bright+ cells expressed low levels of CD 16 but were CD2-. In contrast, most rhesus CD16+ peripheral blood cells were CD56-. In addition to the primary population of CD56bright+ cells, a minor subset of smaller and less granular CD56intermediate+ decidual lymphocytes was identified, the majority of which were CD16-, CD2+. Decidual CD56+ cells did not express monocyte/macrophage markers, including CD14, CD64 and CD68. Decidual lymphocytes effectively lysed K562, Raji and particularly 721.221 targets in cytotoxicity assays. Together, these results suggest that as in human pregnancy, rhesus decidual CD56bright+ cells represent a distinct lymphocyte subset that belongs to the NK cell lineage.

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