Phenotypic and Functional Characterization of Double Negative B Cells in the Blood of Individuals With Obesity

Daniela Frasca, Alain Diaz, Maria Romero, Bonnie B. Blomberg

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously shown that obesity is associated with increased secretion of IgG antibodies with anti-self-reactivity. In this paper, we confirm and extend our previous findings. We show that the plasma of individuals with obesity is enriched in autoimmune antibodies whose levels are positively associated with blood frequencies of the subset of Double Negative (DN) B cells, which is the most pro-inflammatory B cell subset. We also show that DN B cells, significantly increased in the blood of obese versus lean individuals, are characterized by higher expression of immune activation markers and of the transcription factor T-bet, both associated with autoimmunity. The removal of DN B cells from the peripheral B cell pool significantly decreases in vitro secretion of anti-self IgG antibodies. These results altogether confirm the crucial role of DN B cells in the secretion of anti-self IgG antibodies in individuals with obesity.

Original languageEnglish (US)
Article number616650
JournalFrontiers in immunology
Volume12
DOIs
StatePublished - Feb 23 2021

Keywords

  • aging
  • antibody responses
  • B cells
  • inflammation
  • obesity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Phenotypic and Functional Characterization of Double Negative B Cells in the Blood of Individuals With Obesity'. Together they form a unique fingerprint.

Cite this