Pharmacological evidence for A1 and A2 adenosine receptors in the skin microcirculation

I. Stojanov, K. G. Proctor

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

To characterize adenosine-mediated vascular responses, synthetic A1 and A2 receptor agonists (N-ethyl carboxamido adenosine [NECA], 2-chloro adenosine [2CA], or cyclohexyl adenosine [CHA]), the parent compound (adenosine [ADO]), and uptake inhibitor (dipyridamole [DIPYRID]) or a nonselective, competitive antagonist (8-phenyl theophylline [8pTHEO]) were topically applied to 20-60 μm arterioles in the subcutaneous microcirculation of the hamster. Blood flow was calculated from arteriolar diameter and red blood cell velocity using intravital microscopy. At >10-8 M, the potency order for vasodilation (maximum, 170-190% of control) was NECA>2CA>ADO; these responses were attenuated by 10-5 M 8pTHEO. From 10-8 to 10-6 M, 2CA evoked vaosdilation whereas ADO, which has an identical affinity at A1 and A2 receptors, evoked lesser reponses. ADO-induced vasodilation was potentiated by 10-5 M DIPYRID; this response was similar to that evoked by 2CA alone or 2CA+DIPYRID. In contrast to ADO, 2CA is a poor substrate for cellular uptake, which suggests that uptake reduces the A2 effect of exogenous ADO. From 10-10 to 10-8 M, CHA and ADO were equipotent for causing vasoconstriction (minimum, 80-90% of control); these responses were completely antagonzied by 8pTHEO. Norepinephrine was a more potent vasoconstrictor and 8pTHEO did not alter these responses. Since ADO is a metabolic substrate and a nonselective receptor agonist, while CHA is A1-selective and a poor substrate for cellular uptake, neither A2 activation nor cellular uptake altered expression of the A1 effect of exogenous ADO. Furthermore, DIPYRID had no effect on the A1 response. Thus, depending on agonist concentration, stimulation of high affinity A1 receptors or low affinity A2 receptors can cause opposite responses in the skin microcirculation. The precise location or physiological role of these receptors is unknown.

Original languageEnglish (US)
Pages (from-to)176-184
Number of pages9
JournalCirculation research
Volume65
Issue number1
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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