Persistently increased expression of the transforming growth factor-β1 gene in human vascular restenosis: Analysis of 62 patients with one or more episode of restenosis

Sigrid Nikol, Lawrence Weir, Amy Sullivan, Barry Sharaf, Christopher J. White, Gerald Zemel, Geoffrey Hartzler, Richard Stack, Guy Leclerc, Jeffrey M. Isner

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Transforming growth factor-beta-1 (TGF-β1) is a multifunctional cytokine with both growth-promoting and growth-inhibiting properties. Moreover, there is abundant evidence that TGF-β1 is the principal growth factor responsible for regulating proteoglycan synthesis in human blood vessels. To determine the potential contribution of TGF-β1 to restenosis, the current investigation sought to determine the time course of expression postangioplasty of the TGF-β1 gene. In situ hybridization was performed on tissue specimens obtained by directional atherectomy from 62 patients who had previously undergone angioplasty of native coronary or peripheral arteries and/or saphenous vein bypass grafts. The time interval between angioplasty and atherectomy was 1 hour to 25 months (M ± SEM = 5 ± 4 months) for all 62 patients, 5 ± 4 months for coronary arterial specimens, 8 ± 5 months for vein graft specimens, and 7 ± 3 months for peripheral arterial specimens. TGF-β1 mRNA expression remained persistently increased independent of the site from or time interval following which the specimen was obtained. For saphenous vein by pass grafts, TGF-β1 expression was highest in specimens retreived from patients with multiple versus single episodes of restenosis (16 ± 5 vs. 6 ± 5 grains/nucleus, p < 0.01). TGF-β1 expression did not correlate with patient age, sex, or known risk factors for coronary heart disease. The persistently augmented expression of TGF-β1 observed in the present series of restenosis lesions provides further support for the concept that TGF-β1 influences growth and development of restenosis plaque.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalCardiovascular Pathology
Volume3
Issue number1
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Transforming Growth Factors
Transforming Growth Factor beta
Blood Vessels
Genes
Atherectomy
Saphenous Vein
Transplants
Angioplasty
Proteoglycans
Growth
Growth and Development
In Situ Hybridization
Coronary Disease
Veins
Intercellular Signaling Peptides and Proteins
Arteries
Cytokines
Messenger RNA

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pathology and Forensic Medicine

Cite this

Persistently increased expression of the transforming growth factor-β1 gene in human vascular restenosis : Analysis of 62 patients with one or more episode of restenosis. / Nikol, Sigrid; Weir, Lawrence; Sullivan, Amy; Sharaf, Barry; White, Christopher J.; Zemel, Gerald; Hartzler, Geoffrey; Stack, Richard; Leclerc, Guy; Isner, Jeffrey M.

In: Cardiovascular Pathology, Vol. 3, No. 1, 01.01.1994, p. 57-64.

Research output: Contribution to journalArticle

Nikol, Sigrid ; Weir, Lawrence ; Sullivan, Amy ; Sharaf, Barry ; White, Christopher J. ; Zemel, Gerald ; Hartzler, Geoffrey ; Stack, Richard ; Leclerc, Guy ; Isner, Jeffrey M. / Persistently increased expression of the transforming growth factor-β1 gene in human vascular restenosis : Analysis of 62 patients with one or more episode of restenosis. In: Cardiovascular Pathology. 1994 ; Vol. 3, No. 1. pp. 57-64.
@article{ef98795182e547c3be69dc910a14ee10,
title = "Persistently increased expression of the transforming growth factor-β1 gene in human vascular restenosis: Analysis of 62 patients with one or more episode of restenosis",
abstract = "Transforming growth factor-beta-1 (TGF-β1) is a multifunctional cytokine with both growth-promoting and growth-inhibiting properties. Moreover, there is abundant evidence that TGF-β1 is the principal growth factor responsible for regulating proteoglycan synthesis in human blood vessels. To determine the potential contribution of TGF-β1 to restenosis, the current investigation sought to determine the time course of expression postangioplasty of the TGF-β1 gene. In situ hybridization was performed on tissue specimens obtained by directional atherectomy from 62 patients who had previously undergone angioplasty of native coronary or peripheral arteries and/or saphenous vein bypass grafts. The time interval between angioplasty and atherectomy was 1 hour to 25 months (M ± SEM = 5 ± 4 months) for all 62 patients, 5 ± 4 months for coronary arterial specimens, 8 ± 5 months for vein graft specimens, and 7 ± 3 months for peripheral arterial specimens. TGF-β1 mRNA expression remained persistently increased independent of the site from or time interval following which the specimen was obtained. For saphenous vein by pass grafts, TGF-β1 expression was highest in specimens retreived from patients with multiple versus single episodes of restenosis (16 ± 5 vs. 6 ± 5 grains/nucleus, p < 0.01). TGF-β1 expression did not correlate with patient age, sex, or known risk factors for coronary heart disease. The persistently augmented expression of TGF-β1 observed in the present series of restenosis lesions provides further support for the concept that TGF-β1 influences growth and development of restenosis plaque.",
author = "Sigrid Nikol and Lawrence Weir and Amy Sullivan and Barry Sharaf and White, {Christopher J.} and Gerald Zemel and Geoffrey Hartzler and Richard Stack and Guy Leclerc and Isner, {Jeffrey M.}",
year = "1994",
month = "1",
day = "1",
doi = "10.1016/1054-8807(94)90008-6",
language = "English",
volume = "3",
pages = "57--64",
journal = "Cardiovascular Pathology",
issn = "1054-8807",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Persistently increased expression of the transforming growth factor-β1 gene in human vascular restenosis

T2 - Analysis of 62 patients with one or more episode of restenosis

AU - Nikol, Sigrid

AU - Weir, Lawrence

AU - Sullivan, Amy

AU - Sharaf, Barry

AU - White, Christopher J.

AU - Zemel, Gerald

AU - Hartzler, Geoffrey

AU - Stack, Richard

AU - Leclerc, Guy

AU - Isner, Jeffrey M.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Transforming growth factor-beta-1 (TGF-β1) is a multifunctional cytokine with both growth-promoting and growth-inhibiting properties. Moreover, there is abundant evidence that TGF-β1 is the principal growth factor responsible for regulating proteoglycan synthesis in human blood vessels. To determine the potential contribution of TGF-β1 to restenosis, the current investigation sought to determine the time course of expression postangioplasty of the TGF-β1 gene. In situ hybridization was performed on tissue specimens obtained by directional atherectomy from 62 patients who had previously undergone angioplasty of native coronary or peripheral arteries and/or saphenous vein bypass grafts. The time interval between angioplasty and atherectomy was 1 hour to 25 months (M ± SEM = 5 ± 4 months) for all 62 patients, 5 ± 4 months for coronary arterial specimens, 8 ± 5 months for vein graft specimens, and 7 ± 3 months for peripheral arterial specimens. TGF-β1 mRNA expression remained persistently increased independent of the site from or time interval following which the specimen was obtained. For saphenous vein by pass grafts, TGF-β1 expression was highest in specimens retreived from patients with multiple versus single episodes of restenosis (16 ± 5 vs. 6 ± 5 grains/nucleus, p < 0.01). TGF-β1 expression did not correlate with patient age, sex, or known risk factors for coronary heart disease. The persistently augmented expression of TGF-β1 observed in the present series of restenosis lesions provides further support for the concept that TGF-β1 influences growth and development of restenosis plaque.

AB - Transforming growth factor-beta-1 (TGF-β1) is a multifunctional cytokine with both growth-promoting and growth-inhibiting properties. Moreover, there is abundant evidence that TGF-β1 is the principal growth factor responsible for regulating proteoglycan synthesis in human blood vessels. To determine the potential contribution of TGF-β1 to restenosis, the current investigation sought to determine the time course of expression postangioplasty of the TGF-β1 gene. In situ hybridization was performed on tissue specimens obtained by directional atherectomy from 62 patients who had previously undergone angioplasty of native coronary or peripheral arteries and/or saphenous vein bypass grafts. The time interval between angioplasty and atherectomy was 1 hour to 25 months (M ± SEM = 5 ± 4 months) for all 62 patients, 5 ± 4 months for coronary arterial specimens, 8 ± 5 months for vein graft specimens, and 7 ± 3 months for peripheral arterial specimens. TGF-β1 mRNA expression remained persistently increased independent of the site from or time interval following which the specimen was obtained. For saphenous vein by pass grafts, TGF-β1 expression was highest in specimens retreived from patients with multiple versus single episodes of restenosis (16 ± 5 vs. 6 ± 5 grains/nucleus, p < 0.01). TGF-β1 expression did not correlate with patient age, sex, or known risk factors for coronary heart disease. The persistently augmented expression of TGF-β1 observed in the present series of restenosis lesions provides further support for the concept that TGF-β1 influences growth and development of restenosis plaque.

UR - http://www.scopus.com/inward/record.url?scp=0028297443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028297443&partnerID=8YFLogxK

U2 - 10.1016/1054-8807(94)90008-6

DO - 10.1016/1054-8807(94)90008-6

M3 - Article

AN - SCOPUS:0028297443

VL - 3

SP - 57

EP - 64

JO - Cardiovascular Pathology

JF - Cardiovascular Pathology

SN - 1054-8807

IS - 1

ER -