Peroxisome proliferator activated receptors α and γ require zinc for their anti-inflammatory properties in porcine vascular endothelial cells

Gudrun Reiterer, Michal J Toborek, Bernhard Hennig

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Zinc is an essential structural component of various proteins and is crucial for the integrity of the vascular endothelium. The present study focused on the effect of zinc deficiency on the anti-inflammatory properties of peroxisome proliferator activated receptor (PPAR) α and γ agonists. Porcine pulmonary-arterial endothelial cells were deprived from zinc by chelator N,N,N′,N′-tetrakis (2-pyridylmethyl)ethylene diamine. Cells were exposed to TNF-α for 2 h following pretreament with the PPARα agonists fenofibrate or ciprofibrate or the PPARγ agonists thiazolidinedione or troglitazone. The inflammatory response was tested by measuring nuclear factor-kappaB (NF-γB) and activator protein-1 (AP-1) binding activities as well as by measuring mRNA expression levels of inflammatory genes, such as vascular cell adhesion molecule-1 (VCAM-1) and IL-6. All PPAR agonists tested lost their potency to downregulate the TNF-α-induced inflammatory response in zinc-deficient cells. However, if zinc was added back, all PPAR agonists significantly downregulated the TNF-α-mediated induction of inflammatory transcription factors NF-γB and AP-1 and significantly reduced the expression of their target genes, VCAM-1 and IL-6. We therefore hypothesize that zinc is required for the PPARα and -γ DNA binding activity. Indeed, zinc deficiency significantly reduced the agonist-induced binding activity of PPARα and -γ to the PPAR response element. Our data demonstrate the importance of zinc in PPAR signaling and the requirement of zinc for the anti-inflammatory properties of PPARα and -γ agonists.

Original languageEnglish
Pages (from-to)1711-1715
Number of pages5
JournalJournal of Nutrition
Volume134
Issue number7
StatePublished - Jul 1 2004
Externally publishedYes

Fingerprint

Peroxisome Proliferator-Activated Receptors
anti-inflammatory activity
blood vessels
endothelial cells
Zinc
Anti-Inflammatory Agents
Swine
Endothelial Cells
zinc
agonists
swine
transcription factor NF-kappa B
Vascular Cell Adhesion Molecule-1
Transcription Factor AP-1
troglitazone
cell adhesion
interleukin-6
Interleukin-6
Down-Regulation
inflammation

Keywords

  • Atherosclerosis
  • Inflammation
  • PPAR
  • Vascular endothelial cells
  • Zinc

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Peroxisome proliferator activated receptors α and γ require zinc for their anti-inflammatory properties in porcine vascular endothelial cells. / Reiterer, Gudrun; Toborek, Michal J; Hennig, Bernhard.

In: Journal of Nutrition, Vol. 134, No. 7, 01.07.2004, p. 1711-1715.

Research output: Contribution to journalArticle

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