Peripheral gabapentin regulates mosquito allergy-induced itch in mice

Tasuku Akiyama, Tsugunobu Andoh, Eiji Ohtsuka, Hiroshi Nojima, Hidekazu Ouchi, Hiroki Takahata, Yasushi Kuraishi

Research output: Contribution to journalArticle

Abstract

The antipruritic activity of gabapentin, an anticonvulsant, was studied in a mouse model of allergic itch. In mice sensitized by an extract of the salivary glands of the mosquito (ESGM), an intradermal injection of ESGM elicited scratching and increased peripheral nerve firing. Oral or intradermal administration of gabapentin at the ESGM injection site inhibited ESGM-induced scratching and peripheral nerve firing. However, gabapentin did not affect histamine-induced scratching. The distributions of immunoreactivity to the voltage-dependent calcium channel α2δ-1 subunit, a site of gabapentin action, and the histamine H1 receptor differed in the mouse dorsal root ganglia. The α2δ-1 subunit was mainly found in neurons that were 15–20 µm in diameter, whereas the H1 receptor was mainly in 20–30 µm neurons. In addition, α2δ-1 subunit immunoreactivity co-localized with that of transient receptor potential vanilloid 1 (TRPV1). These results suggest that gabapentin regulates allergic itch by acting on the calcium channel α2δ-1 subunit in peripheral TRPV1-positive neurons.

Original languageEnglish (US)
Pages (from-to)44-49
Number of pages6
JournalEuropean Journal of Pharmacology
Volume833
DOIs
StatePublished - Aug 15 2018

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Culicidae
Hypersensitivity
Salivary Glands
Histamine H1 Receptors
Calcium Channels
Peripheral Nerves
Neurons
Antipruritics
Intradermal Injections
Spinal Ganglia
Anticonvulsants
Histamine
gabapentin
Injections
vanilloid receptor subtype 1

Keywords

  • Gabapentin
  • Itch
  • Mosquito allergy
  • Scratch
  • Sensory neuron
  • αδ-1 subunit

ASJC Scopus subject areas

  • Pharmacology

Cite this

Peripheral gabapentin regulates mosquito allergy-induced itch in mice. / Akiyama, Tasuku; Andoh, Tsugunobu; Ohtsuka, Eiji; Nojima, Hiroshi; Ouchi, Hidekazu; Takahata, Hiroki; Kuraishi, Yasushi.

In: European Journal of Pharmacology, Vol. 833, 15.08.2018, p. 44-49.

Research output: Contribution to journalArticle

Akiyama, T, Andoh, T, Ohtsuka, E, Nojima, H, Ouchi, H, Takahata, H & Kuraishi, Y 2018, 'Peripheral gabapentin regulates mosquito allergy-induced itch in mice', European Journal of Pharmacology, vol. 833, pp. 44-49. https://doi.org/10.1016/j.ejphar.2018.05.037
Akiyama, Tasuku ; Andoh, Tsugunobu ; Ohtsuka, Eiji ; Nojima, Hiroshi ; Ouchi, Hidekazu ; Takahata, Hiroki ; Kuraishi, Yasushi. / Peripheral gabapentin regulates mosquito allergy-induced itch in mice. In: European Journal of Pharmacology. 2018 ; Vol. 833. pp. 44-49.
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