Peripheral blood gene expression profiles in metabolic syndrome, coronary artery disease and type 2 diabetes

B. L. Grayson, L. Wang, T. M. Aune

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

To determine if individuals with metabolic disorders possess unique gene expression profiles, we compared transcript levels in peripheral blood from patients with coronary artery disease (CAD), type 2 diabetes (T2D) and their precursor state, metabolic syndrome to those of control (CTRL) subjects and subjects with rheumatoid arthritis (RA). The gene expression profile of each metabolic state was distinguishable from CTRLs and correlated with other metabolic states more than with RA. Of note, subjects in the metabolic cohorts overexpressed gene sets that participate in the innate immune response. Genes involved in activation of the pro-inflammatory transcription factor, NF-B, were overexpressed in CAD whereas genes differentially expressed in T2D have key roles in T-cell activation and signaling. Reverse transcriptase PCR validation confirmed microarray results. Furthermore, several genes differentially expressed in human metabolic disorders have been previously shown to participate in inflammatory responses in murine models of obesity and T2D. Taken together, these data demonstrate that peripheral blood from individuals with metabolic disorders display overlapping and non-overlapping patterns of gene expression indicative of unique, underlying immune processes.

Original languageEnglish (US)
Pages (from-to)341-351
Number of pages11
JournalGenes and Immunity
Volume12
Issue number5
DOIs
StatePublished - Jul 1 2011
Externally publishedYes

Keywords

  • Coronary artery disease
  • gene expression profiles
  • gene set
  • metabolic syndrome
  • rheumatoid arthritis
  • type 2 diabetes

ASJC Scopus subject areas

  • Immunology
  • Genetics
  • Genetics(clinical)

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