Periodontal microbiota and phospholipases: The Oral Infections and Vascular Disease Epidemiology Study (INVEST)

Adrien Boillot, Ryan T. Demmer, Ziad Mallat, Ralph L Sacco, David R. Jacobs, Joelle Benessiano, Alain Tedgui, Tatjana Rundek, Panos N. Papapanou, Moïse Desvarieux

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Objective: Periodontal infections have been linked to cardiovascular disease, including atherosclerosis, and systemic inflammation has been proposed as a possible mediator. Secretory phospholipase A2 (s-PLA2) and Lipoprotein-associated PLA2 (Lp-PLA2) are inflammatory enzymes associated with atherosclerosis. No data are available on the association between oral microbiota and PLA2s. We studied whether a relationship exists between periodontal microbiota and the activities of these enzymes. Methods: The Oral Infection and Vascular Disease Epidemiology Study (INVEST) collected subgingival biofilms and serum samples from 593 dentate men and women (age 68.7 ± 8.6 years). 4561 biofilm samples were collected in the two most posterior teeth of each quadrant (average 7/participant) for quantitative assessment of 11 bacterial species using DNA-DNA checkerboard hybridization. Mean concentration of s-PLA2 and activities of s-PLA2 and Lp-PLA2 were regressed on tertiles of etiologic dominance (ED). ED is defined as the level of presumed periodontopathic species/combined level of all eleven species measured, and represents the relative abundance of periodontopathic organisms. Analyses were adjusted for age, sex, race/ethnicity, education, smoking, BMI, diabetes, LDL cholesterol and HDL cholesterol, and systolic blood pressure. Results: Higher levels of s-PLA2 activity were observed across increasing tertiles of etiologic dominance (0.66 ± 0.04 nmol ml<sup>-1</sup> min<sup>-1</sup>, 0.73 ± 0.04 nmol ml<sup>-1</sup> min<sup>-1</sup>, 0.89 ± 0.04 nmol ml-1 min-1; p < 0.001), with also a trend of association between Lp-PLA2 activity and ED (p = 0.07), while s-PLA2 concentration was unrelated to ED. Conclusion: Increasingly greater s-PLA2 activity at higher tertiles of etiologic dominance may provide a mechanistic explanatory link of the relationship between periodontal microbiota and vascular diseases. Additional studies investigating the role of s-PLA2 are needed.

Original languageEnglish (US)
Pages (from-to)418-423
Number of pages6
JournalAtherosclerosis
Volume242
Issue number2
DOIs
StatePublished - Oct 1 2015

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Keywords

  • Atherosclerosis
  • Cardiovascular diseases
  • Periodontitis
  • Phospholipases

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Boillot, A., Demmer, R. T., Mallat, Z., Sacco, R. L., Jacobs, D. R., Benessiano, J., Tedgui, A., Rundek, T., Papapanou, P. N., & Desvarieux, M. (2015). Periodontal microbiota and phospholipases: The Oral Infections and Vascular Disease Epidemiology Study (INVEST). Atherosclerosis, 242(2), 418-423. https://doi.org/10.1016/j.atherosclerosis.2015.07.039