Periodontal microbiota and phospholipases: The Oral Infections and Vascular Disease Epidemiology Study (INVEST)

Adrien Boillot, Ryan T. Demmer, Ziad Mallat, Ralph L Sacco, David R. Jacobs, Joelle Benessiano, Alain Tedgui, Tatjana Rundek, Panos N. Papapanou, Moïse Desvarieux

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: Periodontal infections have been linked to cardiovascular disease, including atherosclerosis, and systemic inflammation has been proposed as a possible mediator. Secretory phospholipase A2 (s-PLA2) and Lipoprotein-associated PLA2 (Lp-PLA2) are inflammatory enzymes associated with atherosclerosis. No data are available on the association between oral microbiota and PLA2s. We studied whether a relationship exists between periodontal microbiota and the activities of these enzymes. Methods: The Oral Infection and Vascular Disease Epidemiology Study (INVEST) collected subgingival biofilms and serum samples from 593 dentate men and women (age 68.7 ± 8.6 years). 4561 biofilm samples were collected in the two most posterior teeth of each quadrant (average 7/participant) for quantitative assessment of 11 bacterial species using DNA-DNA checkerboard hybridization. Mean concentration of s-PLA2 and activities of s-PLA2 and Lp-PLA2 were regressed on tertiles of etiologic dominance (ED). ED is defined as the level of presumed periodontopathic species/combined level of all eleven species measured, and represents the relative abundance of periodontopathic organisms. Analyses were adjusted for age, sex, race/ethnicity, education, smoking, BMI, diabetes, LDL cholesterol and HDL cholesterol, and systolic blood pressure. Results: Higher levels of s-PLA2 activity were observed across increasing tertiles of etiologic dominance (0.66 ± 0.04 nmol ml<sup>-1</sup> min<sup>-1</sup>, 0.73 ± 0.04 nmol ml<sup>-1</sup> min<sup>-1</sup>, 0.89 ± 0.04 nmol ml-1 min-1; p < 0.001), with also a trend of association between Lp-PLA2 activity and ED (p = 0.07), while s-PLA2 concentration was unrelated to ED. Conclusion: Increasingly greater s-PLA2 activity at higher tertiles of etiologic dominance may provide a mechanistic explanatory link of the relationship between periodontal microbiota and vascular diseases. Additional studies investigating the role of s-PLA2 are needed.

Original languageEnglish (US)
Pages (from-to)418-423
Number of pages6
JournalAtherosclerosis
Volume242
Issue number2
DOIs
StatePublished - Oct 1 2015

Fingerprint

Mouth Diseases
Secretory Phospholipase A2
Phospholipases
Microbiota
Vascular Diseases
Epidemiology
Infection
Lipoproteins
Biofilms
Atherosclerosis
Blood Pressure
DNA
Periodontal Diseases
Enzymes
LDL Cholesterol
HDL Cholesterol
Tooth
Cardiovascular Diseases
Smoking
Inflammation

Keywords

  • Atherosclerosis
  • Cardiovascular diseases
  • Periodontitis
  • Phospholipases

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Periodontal microbiota and phospholipases : The Oral Infections and Vascular Disease Epidemiology Study (INVEST). / Boillot, Adrien; Demmer, Ryan T.; Mallat, Ziad; Sacco, Ralph L; Jacobs, David R.; Benessiano, Joelle; Tedgui, Alain; Rundek, Tatjana; Papapanou, Panos N.; Desvarieux, Moïse.

In: Atherosclerosis, Vol. 242, No. 2, 01.10.2015, p. 418-423.

Research output: Contribution to journalArticle

Boillot, A, Demmer, RT, Mallat, Z, Sacco, RL, Jacobs, DR, Benessiano, J, Tedgui, A, Rundek, T, Papapanou, PN & Desvarieux, M 2015, 'Periodontal microbiota and phospholipases: The Oral Infections and Vascular Disease Epidemiology Study (INVEST)', Atherosclerosis, vol. 242, no. 2, pp. 418-423. https://doi.org/10.1016/j.atherosclerosis.2015.07.039
Boillot, Adrien ; Demmer, Ryan T. ; Mallat, Ziad ; Sacco, Ralph L ; Jacobs, David R. ; Benessiano, Joelle ; Tedgui, Alain ; Rundek, Tatjana ; Papapanou, Panos N. ; Desvarieux, Moïse. / Periodontal microbiota and phospholipases : The Oral Infections and Vascular Disease Epidemiology Study (INVEST). In: Atherosclerosis. 2015 ; Vol. 242, No. 2. pp. 418-423.
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abstract = "Objective: Periodontal infections have been linked to cardiovascular disease, including atherosclerosis, and systemic inflammation has been proposed as a possible mediator. Secretory phospholipase A2 (s-PLA2) and Lipoprotein-associated PLA2 (Lp-PLA2) are inflammatory enzymes associated with atherosclerosis. No data are available on the association between oral microbiota and PLA2s. We studied whether a relationship exists between periodontal microbiota and the activities of these enzymes. Methods: The Oral Infection and Vascular Disease Epidemiology Study (INVEST) collected subgingival biofilms and serum samples from 593 dentate men and women (age 68.7 ± 8.6 years). 4561 biofilm samples were collected in the two most posterior teeth of each quadrant (average 7/participant) for quantitative assessment of 11 bacterial species using DNA-DNA checkerboard hybridization. Mean concentration of s-PLA2 and activities of s-PLA2 and Lp-PLA2 were regressed on tertiles of etiologic dominance (ED). ED is defined as the level of presumed periodontopathic species/combined level of all eleven species measured, and represents the relative abundance of periodontopathic organisms. Analyses were adjusted for age, sex, race/ethnicity, education, smoking, BMI, diabetes, LDL cholesterol and HDL cholesterol, and systolic blood pressure. Results: Higher levels of s-PLA2 activity were observed across increasing tertiles of etiologic dominance (0.66 ± 0.04 nmol ml-1 min-1, 0.73 ± 0.04 nmol ml-1 min-1, 0.89 ± 0.04 nmol ml-1 min-1; p < 0.001), with also a trend of association between Lp-PLA2 activity and ED (p = 0.07), while s-PLA2 concentration was unrelated to ED. Conclusion: Increasingly greater s-PLA2 activity at higher tertiles of etiologic dominance may provide a mechanistic explanatory link of the relationship between periodontal microbiota and vascular diseases. Additional studies investigating the role of s-PLA2 are needed.",
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AU - Mallat, Ziad

AU - Sacco, Ralph L

AU - Jacobs, David R.

AU - Benessiano, Joelle

AU - Tedgui, Alain

AU - Rundek, Tatjana

AU - Papapanou, Panos N.

AU - Desvarieux, Moïse

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N2 - Objective: Periodontal infections have been linked to cardiovascular disease, including atherosclerosis, and systemic inflammation has been proposed as a possible mediator. Secretory phospholipase A2 (s-PLA2) and Lipoprotein-associated PLA2 (Lp-PLA2) are inflammatory enzymes associated with atherosclerosis. No data are available on the association between oral microbiota and PLA2s. We studied whether a relationship exists between periodontal microbiota and the activities of these enzymes. Methods: The Oral Infection and Vascular Disease Epidemiology Study (INVEST) collected subgingival biofilms and serum samples from 593 dentate men and women (age 68.7 ± 8.6 years). 4561 biofilm samples were collected in the two most posterior teeth of each quadrant (average 7/participant) for quantitative assessment of 11 bacterial species using DNA-DNA checkerboard hybridization. Mean concentration of s-PLA2 and activities of s-PLA2 and Lp-PLA2 were regressed on tertiles of etiologic dominance (ED). ED is defined as the level of presumed periodontopathic species/combined level of all eleven species measured, and represents the relative abundance of periodontopathic organisms. Analyses were adjusted for age, sex, race/ethnicity, education, smoking, BMI, diabetes, LDL cholesterol and HDL cholesterol, and systolic blood pressure. Results: Higher levels of s-PLA2 activity were observed across increasing tertiles of etiologic dominance (0.66 ± 0.04 nmol ml-1 min-1, 0.73 ± 0.04 nmol ml-1 min-1, 0.89 ± 0.04 nmol ml-1 min-1; p < 0.001), with also a trend of association between Lp-PLA2 activity and ED (p = 0.07), while s-PLA2 concentration was unrelated to ED. Conclusion: Increasingly greater s-PLA2 activity at higher tertiles of etiologic dominance may provide a mechanistic explanatory link of the relationship between periodontal microbiota and vascular diseases. Additional studies investigating the role of s-PLA2 are needed.

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