Perineurium originates from fibroblasts

Demonstration in vitro with a retroviral marker

Mary B Bunge, Patrick Wood, L. B. Tynan, M. L. Bates, J. R. Sanes

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

A cellular sheath, the perineurium, forms a protective barrier around fascicles of nerve fibers throughout the peripheral nervous system. In a study to determine the cellular origin of perineurium, a culture system was used in which perineurium forms after purified populations of sensory neurons, Schwann cells, and fibroblasts are recombined. Before recombination, the Schwann cells or the fibroblasts were labeled by infection with a defective recombinant retrovirus whose gene product, β-galactosidase, is histochemically detectable in the progeny of infected cells. Perineurial cells were labeled when fibroblasts had been infected but not when Schwann cells had been infected. Thus, perineurium arises from fibroblasts in vitro and, by implication, in vivo as well.

Original languageEnglish
Pages (from-to)229-231
Number of pages3
JournalScience
Volume243
Issue number4888
StatePublished - Jan 1 1989
Externally publishedYes

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Peripheral Nerves
Schwann Cells
Fibroblasts
Galactosidases
Peripheral Nervous System
Sensory Receptor Cells
Retroviridae
Nerve Fibers
Genetic Recombination
In Vitro Techniques
Infection
Population
Genes

ASJC Scopus subject areas

  • General

Cite this

Bunge, M. B., Wood, P., Tynan, L. B., Bates, M. L., & Sanes, J. R. (1989). Perineurium originates from fibroblasts: Demonstration in vitro with a retroviral marker. Science, 243(4888), 229-231.

Perineurium originates from fibroblasts : Demonstration in vitro with a retroviral marker. / Bunge, Mary B; Wood, Patrick; Tynan, L. B.; Bates, M. L.; Sanes, J. R.

In: Science, Vol. 243, No. 4888, 01.01.1989, p. 229-231.

Research output: Contribution to journalArticle

Bunge, MB, Wood, P, Tynan, LB, Bates, ML & Sanes, JR 1989, 'Perineurium originates from fibroblasts: Demonstration in vitro with a retroviral marker', Science, vol. 243, no. 4888, pp. 229-231.
Bunge MB, Wood P, Tynan LB, Bates ML, Sanes JR. Perineurium originates from fibroblasts: Demonstration in vitro with a retroviral marker. Science. 1989 Jan 1;243(4888):229-231.
Bunge, Mary B ; Wood, Patrick ; Tynan, L. B. ; Bates, M. L. ; Sanes, J. R. / Perineurium originates from fibroblasts : Demonstration in vitro with a retroviral marker. In: Science. 1989 ; Vol. 243, No. 4888. pp. 229-231.
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