TY - JOUR
T1 - Perineurium originates from fibroblasts
T2 - Demonstration in vitro with a retroviral marker
AU - Bunge, Mary Bartlett
AU - Wood, Patrick M.
AU - Tynan, Laura B.
AU - Bates, Margaret L.
AU - Sanes, Joshua R.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1989
Y1 - 1989
N2 - A cellular sheath, the perineurium, forms a protective barrier around fascicles of nerve fibers throughout the peripheral nervous system. In a study to determine the cellular origin of perineurium, a culture system was used in which perineurium forms after purified populations of sensory neurons, Schwann cells, and fibroblasts are recombined. Before recombination, the Schwann cells or the fibroblasts were labeled by infection with a defective recombinant retrovirus whose gene product, β-galactosidase, is histochemically detectable in the progeny of infected cells. Perineurial cells were labeled when fibroblasts had been infected but not when Schwann cells had been infected. Thus, perineurium arises from fibroblasts in vitro and, by implication, in vivo as well.
AB - A cellular sheath, the perineurium, forms a protective barrier around fascicles of nerve fibers throughout the peripheral nervous system. In a study to determine the cellular origin of perineurium, a culture system was used in which perineurium forms after purified populations of sensory neurons, Schwann cells, and fibroblasts are recombined. Before recombination, the Schwann cells or the fibroblasts were labeled by infection with a defective recombinant retrovirus whose gene product, β-galactosidase, is histochemically detectable in the progeny of infected cells. Perineurial cells were labeled when fibroblasts had been infected but not when Schwann cells had been infected. Thus, perineurium arises from fibroblasts in vitro and, by implication, in vivo as well.
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U2 - 10.1126/science.2492115
DO - 10.1126/science.2492115
M3 - Article
C2 - 2492115
AN - SCOPUS:0024540881
VL - 243
SP - 229
EP - 231
JO - Science
JF - Science
SN - 0036-8075
IS - 4888
ER -