Performance of two-dimensional Doppler echocardiography for the assessment of infarct size and left ventricular function in rats

E. Nozawa, Rosemeire Takeuchi, N. Murad, A. C C Carvalho, S. L D Cravo, O. Campos, P. J F Tucci, V. A. Moises

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Although echocardiography has been used in rats, few studies have determined its efficacy for estimating myocardial infarct size. Our objective was to estimate the myocardial infarct size, and to evaluate anatomic and functional variables of the left ventricle. Myocardial infarction was produced in 43 female Wistar rats by ligature of the left coronary artery. Echocardiography was performed 5 weeks later to measure left ventricular diameter and transverse area (mean of 3 transverse planes), infarct size (percentage of the arc with infarct on 3 transverse planes), systolic function by the change in fractional area, and diastolic function by mitral inflow parameters. The histologic measurement of myocardial infarction size was similar to the echocardiographic method. Myocardial infarct size ranged from 4.8 to 66.6% when determined by histology and from 5 to 69.8% when determined by echocardiography, with good correlation (r = 0.88; P <0.05; Pearson correlation coefficient). Left ventricular diameter and mean diastolic transverse area correlated with myocardial infarct size by histology (r = 0.57 and r = 0.78; P <0.0005). The fractional area change ranged from 28.5 ± 5.6 (large-size myocardial infarction) to 53.1 ± 1.5% (control) and correlated with myocardial infarct size by echocardiography (r = -0.87; P <0.00001) and histology (r = -0.78; P <00001). The E/A wave ratio of mitral inflow velocity for animals with large-size myocardial infarction (5.6 ± 2.7) was significantly higher than for all others (control: 1.9 ± 0.1; small-size myocardial infarction: 1.9 ± 0.4; moderate-size myocardial infarction: 2.8 ± 2.3). There was good agreement between echocardiographic and histologic estimates of myocardial infarct size in rats.

Original languageEnglish (US)
Pages (from-to)687-695
Number of pages9
JournalBrazilian Journal of Medical and Biological Research
Volume39
Issue number5
DOIs
StatePublished - May 2006
Externally publishedYes

Fingerprint

Echocardiography
Doppler Echocardiography
infarction
myocardial infarction
echocardiography
Left Ventricular Function
Histology
Rats
Myocardial Infarction
rats
histology
Animals
coronary vessels
Heart Ventricles
Ligation
Wistar Rats
Coronary Vessels

Keywords

  • Doppler echocardiography
  • Myocardial infarction size
  • Rats
  • Systolic and diastolic function

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Medicine (miscellaneous)

Cite this

Performance of two-dimensional Doppler echocardiography for the assessment of infarct size and left ventricular function in rats. / Nozawa, E.; Takeuchi, Rosemeire; Murad, N.; Carvalho, A. C C; Cravo, S. L D; Campos, O.; Tucci, P. J F; Moises, V. A.

In: Brazilian Journal of Medical and Biological Research, Vol. 39, No. 5, 05.2006, p. 687-695.

Research output: Contribution to journalArticle

Nozawa, E. ; Takeuchi, Rosemeire ; Murad, N. ; Carvalho, A. C C ; Cravo, S. L D ; Campos, O. ; Tucci, P. J F ; Moises, V. A. / Performance of two-dimensional Doppler echocardiography for the assessment of infarct size and left ventricular function in rats. In: Brazilian Journal of Medical and Biological Research. 2006 ; Vol. 39, No. 5. pp. 687-695.
@article{0fac80e20a7646ad9d60f1df19f1d8fa,
title = "Performance of two-dimensional Doppler echocardiography for the assessment of infarct size and left ventricular function in rats",
abstract = "Although echocardiography has been used in rats, few studies have determined its efficacy for estimating myocardial infarct size. Our objective was to estimate the myocardial infarct size, and to evaluate anatomic and functional variables of the left ventricle. Myocardial infarction was produced in 43 female Wistar rats by ligature of the left coronary artery. Echocardiography was performed 5 weeks later to measure left ventricular diameter and transverse area (mean of 3 transverse planes), infarct size (percentage of the arc with infarct on 3 transverse planes), systolic function by the change in fractional area, and diastolic function by mitral inflow parameters. The histologic measurement of myocardial infarction size was similar to the echocardiographic method. Myocardial infarct size ranged from 4.8 to 66.6{\%} when determined by histology and from 5 to 69.8{\%} when determined by echocardiography, with good correlation (r = 0.88; P <0.05; Pearson correlation coefficient). Left ventricular diameter and mean diastolic transverse area correlated with myocardial infarct size by histology (r = 0.57 and r = 0.78; P <0.0005). The fractional area change ranged from 28.5 ± 5.6 (large-size myocardial infarction) to 53.1 ± 1.5{\%} (control) and correlated with myocardial infarct size by echocardiography (r = -0.87; P <0.00001) and histology (r = -0.78; P <00001). The E/A wave ratio of mitral inflow velocity for animals with large-size myocardial infarction (5.6 ± 2.7) was significantly higher than for all others (control: 1.9 ± 0.1; small-size myocardial infarction: 1.9 ± 0.4; moderate-size myocardial infarction: 2.8 ± 2.3). There was good agreement between echocardiographic and histologic estimates of myocardial infarct size in rats.",
keywords = "Doppler echocardiography, Myocardial infarction size, Rats, Systolic and diastolic function",
author = "E. Nozawa and Rosemeire Takeuchi and N. Murad and Carvalho, {A. C C} and Cravo, {S. L D} and O. Campos and Tucci, {P. J F} and Moises, {V. A.}",
year = "2006",
month = "5",
doi = "10.1590/S0100-879X2006000500016",
language = "English (US)",
volume = "39",
pages = "687--695",
journal = "Brazilian Journal of Medical and Biological Research",
issn = "0100-879X",
publisher = "Associacao Brasileira de Divulgacao Cientifica",
number = "5",

}

TY - JOUR

T1 - Performance of two-dimensional Doppler echocardiography for the assessment of infarct size and left ventricular function in rats

AU - Nozawa, E.

AU - Takeuchi, Rosemeire

AU - Murad, N.

AU - Carvalho, A. C C

AU - Cravo, S. L D

AU - Campos, O.

AU - Tucci, P. J F

AU - Moises, V. A.

PY - 2006/5

Y1 - 2006/5

N2 - Although echocardiography has been used in rats, few studies have determined its efficacy for estimating myocardial infarct size. Our objective was to estimate the myocardial infarct size, and to evaluate anatomic and functional variables of the left ventricle. Myocardial infarction was produced in 43 female Wistar rats by ligature of the left coronary artery. Echocardiography was performed 5 weeks later to measure left ventricular diameter and transverse area (mean of 3 transverse planes), infarct size (percentage of the arc with infarct on 3 transverse planes), systolic function by the change in fractional area, and diastolic function by mitral inflow parameters. The histologic measurement of myocardial infarction size was similar to the echocardiographic method. Myocardial infarct size ranged from 4.8 to 66.6% when determined by histology and from 5 to 69.8% when determined by echocardiography, with good correlation (r = 0.88; P <0.05; Pearson correlation coefficient). Left ventricular diameter and mean diastolic transverse area correlated with myocardial infarct size by histology (r = 0.57 and r = 0.78; P <0.0005). The fractional area change ranged from 28.5 ± 5.6 (large-size myocardial infarction) to 53.1 ± 1.5% (control) and correlated with myocardial infarct size by echocardiography (r = -0.87; P <0.00001) and histology (r = -0.78; P <00001). The E/A wave ratio of mitral inflow velocity for animals with large-size myocardial infarction (5.6 ± 2.7) was significantly higher than for all others (control: 1.9 ± 0.1; small-size myocardial infarction: 1.9 ± 0.4; moderate-size myocardial infarction: 2.8 ± 2.3). There was good agreement between echocardiographic and histologic estimates of myocardial infarct size in rats.

AB - Although echocardiography has been used in rats, few studies have determined its efficacy for estimating myocardial infarct size. Our objective was to estimate the myocardial infarct size, and to evaluate anatomic and functional variables of the left ventricle. Myocardial infarction was produced in 43 female Wistar rats by ligature of the left coronary artery. Echocardiography was performed 5 weeks later to measure left ventricular diameter and transverse area (mean of 3 transverse planes), infarct size (percentage of the arc with infarct on 3 transverse planes), systolic function by the change in fractional area, and diastolic function by mitral inflow parameters. The histologic measurement of myocardial infarction size was similar to the echocardiographic method. Myocardial infarct size ranged from 4.8 to 66.6% when determined by histology and from 5 to 69.8% when determined by echocardiography, with good correlation (r = 0.88; P <0.05; Pearson correlation coefficient). Left ventricular diameter and mean diastolic transverse area correlated with myocardial infarct size by histology (r = 0.57 and r = 0.78; P <0.0005). The fractional area change ranged from 28.5 ± 5.6 (large-size myocardial infarction) to 53.1 ± 1.5% (control) and correlated with myocardial infarct size by echocardiography (r = -0.87; P <0.00001) and histology (r = -0.78; P <00001). The E/A wave ratio of mitral inflow velocity for animals with large-size myocardial infarction (5.6 ± 2.7) was significantly higher than for all others (control: 1.9 ± 0.1; small-size myocardial infarction: 1.9 ± 0.4; moderate-size myocardial infarction: 2.8 ± 2.3). There was good agreement between echocardiographic and histologic estimates of myocardial infarct size in rats.

KW - Doppler echocardiography

KW - Myocardial infarction size

KW - Rats

KW - Systolic and diastolic function

UR - http://www.scopus.com/inward/record.url?scp=33646418980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646418980&partnerID=8YFLogxK

U2 - 10.1590/S0100-879X2006000500016

DO - 10.1590/S0100-879X2006000500016

M3 - Article

C2 - 16648907

AN - SCOPUS:33646418980

VL - 39

SP - 687

EP - 695

JO - Brazilian Journal of Medical and Biological Research

JF - Brazilian Journal of Medical and Biological Research

SN - 0100-879X

IS - 5

ER -