Performance of the cepheid CT/NG Xpert rapid PCR test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae

Charlotte A. Gaydos, Barbara Van Der Pol, Mary Jett-Goheen, Mathilda Barnes, Nicole Quinn, Carey Clark, Grace E. Daniel, Paula B. Dixon, Edward W. Hook, Thomas Davis, Preeti Pancholi, Peter Kerndt, Patrick Horner, Jeanne Marrazzo, Andrew De Burgh-Thomas, Jose G Castro, Dorothy Ferguson, Michelle Meyer, Susan Philip, Bobbie Van Der Pol & 25 others Melanie Thompson, Stephanie Taylor, David Ronk, Paul Fine, Laura Bachman, Donna Mayne, Craig Dietz, Kim Toevs, Nikole Gettings, Stanley Gall, Rajul Patel, Jonathan Ross, Latina Brooks, Lydie Hazan, Patrick Clarke, Robert Groom, Ronald Ackerman, Eugene Anduczyk, Anakutty Mathews, Stephen Blank, Lisa Harris, Albert Tidings, Philippa Moore, Samuel Alexander, Timothy Spurrell

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Tests for Chlamydia trachomatis and Neisseria gonorrhoeae, which can provide results rapidly to guide therapeutic decision-making, offer patient care advantages over laboratory-based tests that require several days to provide results.Wecompared results from the Cepheid GeneXpert CT/NG (Xpert) assay to results from two currently approved nucleic acid amplification assays in 1,722 female and 1,387 male volunteers. Results for chlamydia in females demonstrated sensitivities for endocervical, vaginal, and urine samples of 97.4%, 98.7%, and 97.6%, respectively, and for urine samples from males, a sensitivity of 97.5%, with all specificity estimates being ≥99.4%. Results for gonorrhea in females demonstrated sensitivities for endocervical, vaginal, and urine samples of 100.0%, 100.0%, and 95.6%, respectively, and for urine samples from males, a sensitivity of 98.0%, with all estimates of specificity being ≥99.8%. These results indicate that this short-turnaround-time test can be used to accurately test patients and to possibly do so at the site of care, thus potentially improving chlamydia and gonorrhea control efforts.

Original languageEnglish
Pages (from-to)1666-1672
Number of pages7
JournalJournal of Clinical Microbiology
Volume51
Issue number6
DOIs
StatePublished - Jun 1 2013

Fingerprint

Neisseria gonorrhoeae
Chlamydia trachomatis
Urine
Polymerase Chain Reaction
Chlamydia
Gonorrhea
Nucleic Acids
Volunteers
Decision Making
Patient Care
Therapeutics

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Gaydos, C. A., Van Der Pol, B., Jett-Goheen, M., Barnes, M., Quinn, N., Clark, C., ... Spurrell, T. (2013). Performance of the cepheid CT/NG Xpert rapid PCR test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Journal of Clinical Microbiology, 51(6), 1666-1672. https://doi.org/10.1128/JCM.03461-12

Performance of the cepheid CT/NG Xpert rapid PCR test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. / Gaydos, Charlotte A.; Van Der Pol, Barbara; Jett-Goheen, Mary; Barnes, Mathilda; Quinn, Nicole; Clark, Carey; Daniel, Grace E.; Dixon, Paula B.; Hook, Edward W.; Davis, Thomas; Pancholi, Preeti; Kerndt, Peter; Horner, Patrick; Marrazzo, Jeanne; De Burgh-Thomas, Andrew; Castro, Jose G; Ferguson, Dorothy; Meyer, Michelle; Philip, Susan; Van Der Pol, Bobbie; Thompson, Melanie; Taylor, Stephanie; Ronk, David; Fine, Paul; Bachman, Laura; Mayne, Donna; Dietz, Craig; Toevs, Kim; Gettings, Nikole; Gall, Stanley; Patel, Rajul; Ross, Jonathan; Brooks, Latina; Hazan, Lydie; Clarke, Patrick; Groom, Robert; Ackerman, Ronald; Anduczyk, Eugene; Mathews, Anakutty; Blank, Stephen; Harris, Lisa; Tidings, Albert; Moore, Philippa; Alexander, Samuel; Spurrell, Timothy.

In: Journal of Clinical Microbiology, Vol. 51, No. 6, 01.06.2013, p. 1666-1672.

Research output: Contribution to journalArticle

Gaydos, CA, Van Der Pol, B, Jett-Goheen, M, Barnes, M, Quinn, N, Clark, C, Daniel, GE, Dixon, PB, Hook, EW, Davis, T, Pancholi, P, Kerndt, P, Horner, P, Marrazzo, J, De Burgh-Thomas, A, Castro, JG, Ferguson, D, Meyer, M, Philip, S, Van Der Pol, B, Thompson, M, Taylor, S, Ronk, D, Fine, P, Bachman, L, Mayne, D, Dietz, C, Toevs, K, Gettings, N, Gall, S, Patel, R, Ross, J, Brooks, L, Hazan, L, Clarke, P, Groom, R, Ackerman, R, Anduczyk, E, Mathews, A, Blank, S, Harris, L, Tidings, A, Moore, P, Alexander, S & Spurrell, T 2013, 'Performance of the cepheid CT/NG Xpert rapid PCR test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae', Journal of Clinical Microbiology, vol. 51, no. 6, pp. 1666-1672. https://doi.org/10.1128/JCM.03461-12
Gaydos, Charlotte A. ; Van Der Pol, Barbara ; Jett-Goheen, Mary ; Barnes, Mathilda ; Quinn, Nicole ; Clark, Carey ; Daniel, Grace E. ; Dixon, Paula B. ; Hook, Edward W. ; Davis, Thomas ; Pancholi, Preeti ; Kerndt, Peter ; Horner, Patrick ; Marrazzo, Jeanne ; De Burgh-Thomas, Andrew ; Castro, Jose G ; Ferguson, Dorothy ; Meyer, Michelle ; Philip, Susan ; Van Der Pol, Bobbie ; Thompson, Melanie ; Taylor, Stephanie ; Ronk, David ; Fine, Paul ; Bachman, Laura ; Mayne, Donna ; Dietz, Craig ; Toevs, Kim ; Gettings, Nikole ; Gall, Stanley ; Patel, Rajul ; Ross, Jonathan ; Brooks, Latina ; Hazan, Lydie ; Clarke, Patrick ; Groom, Robert ; Ackerman, Ronald ; Anduczyk, Eugene ; Mathews, Anakutty ; Blank, Stephen ; Harris, Lisa ; Tidings, Albert ; Moore, Philippa ; Alexander, Samuel ; Spurrell, Timothy. / Performance of the cepheid CT/NG Xpert rapid PCR test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. In: Journal of Clinical Microbiology. 2013 ; Vol. 51, No. 6. pp. 1666-1672.
@article{e7faf01e94934270b066a3cbd45adc6c,
title = "Performance of the cepheid CT/NG Xpert rapid PCR test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae",
abstract = "Tests for Chlamydia trachomatis and Neisseria gonorrhoeae, which can provide results rapidly to guide therapeutic decision-making, offer patient care advantages over laboratory-based tests that require several days to provide results.Wecompared results from the Cepheid GeneXpert CT/NG (Xpert) assay to results from two currently approved nucleic acid amplification assays in 1,722 female and 1,387 male volunteers. Results for chlamydia in females demonstrated sensitivities for endocervical, vaginal, and urine samples of 97.4{\%}, 98.7{\%}, and 97.6{\%}, respectively, and for urine samples from males, a sensitivity of 97.5{\%}, with all specificity estimates being ≥99.4{\%}. Results for gonorrhea in females demonstrated sensitivities for endocervical, vaginal, and urine samples of 100.0{\%}, 100.0{\%}, and 95.6{\%}, respectively, and for urine samples from males, a sensitivity of 98.0{\%}, with all estimates of specificity being ≥99.8{\%}. These results indicate that this short-turnaround-time test can be used to accurately test patients and to possibly do so at the site of care, thus potentially improving chlamydia and gonorrhea control efforts.",
author = "Gaydos, {Charlotte A.} and {Van Der Pol}, Barbara and Mary Jett-Goheen and Mathilda Barnes and Nicole Quinn and Carey Clark and Daniel, {Grace E.} and Dixon, {Paula B.} and Hook, {Edward W.} and Thomas Davis and Preeti Pancholi and Peter Kerndt and Patrick Horner and Jeanne Marrazzo and {De Burgh-Thomas}, Andrew and Castro, {Jose G} and Dorothy Ferguson and Michelle Meyer and Susan Philip and {Van Der Pol}, Bobbie and Melanie Thompson and Stephanie Taylor and David Ronk and Paul Fine and Laura Bachman and Donna Mayne and Craig Dietz and Kim Toevs and Nikole Gettings and Stanley Gall and Rajul Patel and Jonathan Ross and Latina Brooks and Lydie Hazan and Patrick Clarke and Robert Groom and Ronald Ackerman and Eugene Anduczyk and Anakutty Mathews and Stephen Blank and Lisa Harris and Albert Tidings and Philippa Moore and Samuel Alexander and Timothy Spurrell",
year = "2013",
month = "6",
day = "1",
doi = "10.1128/JCM.03461-12",
language = "English",
volume = "51",
pages = "1666--1672",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Performance of the cepheid CT/NG Xpert rapid PCR test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae

AU - Gaydos, Charlotte A.

AU - Van Der Pol, Barbara

AU - Jett-Goheen, Mary

AU - Barnes, Mathilda

AU - Quinn, Nicole

AU - Clark, Carey

AU - Daniel, Grace E.

AU - Dixon, Paula B.

AU - Hook, Edward W.

AU - Davis, Thomas

AU - Pancholi, Preeti

AU - Kerndt, Peter

AU - Horner, Patrick

AU - Marrazzo, Jeanne

AU - De Burgh-Thomas, Andrew

AU - Castro, Jose G

AU - Ferguson, Dorothy

AU - Meyer, Michelle

AU - Philip, Susan

AU - Van Der Pol, Bobbie

AU - Thompson, Melanie

AU - Taylor, Stephanie

AU - Ronk, David

AU - Fine, Paul

AU - Bachman, Laura

AU - Mayne, Donna

AU - Dietz, Craig

AU - Toevs, Kim

AU - Gettings, Nikole

AU - Gall, Stanley

AU - Patel, Rajul

AU - Ross, Jonathan

AU - Brooks, Latina

AU - Hazan, Lydie

AU - Clarke, Patrick

AU - Groom, Robert

AU - Ackerman, Ronald

AU - Anduczyk, Eugene

AU - Mathews, Anakutty

AU - Blank, Stephen

AU - Harris, Lisa

AU - Tidings, Albert

AU - Moore, Philippa

AU - Alexander, Samuel

AU - Spurrell, Timothy

PY - 2013/6/1

Y1 - 2013/6/1

N2 - Tests for Chlamydia trachomatis and Neisseria gonorrhoeae, which can provide results rapidly to guide therapeutic decision-making, offer patient care advantages over laboratory-based tests that require several days to provide results.Wecompared results from the Cepheid GeneXpert CT/NG (Xpert) assay to results from two currently approved nucleic acid amplification assays in 1,722 female and 1,387 male volunteers. Results for chlamydia in females demonstrated sensitivities for endocervical, vaginal, and urine samples of 97.4%, 98.7%, and 97.6%, respectively, and for urine samples from males, a sensitivity of 97.5%, with all specificity estimates being ≥99.4%. Results for gonorrhea in females demonstrated sensitivities for endocervical, vaginal, and urine samples of 100.0%, 100.0%, and 95.6%, respectively, and for urine samples from males, a sensitivity of 98.0%, with all estimates of specificity being ≥99.8%. These results indicate that this short-turnaround-time test can be used to accurately test patients and to possibly do so at the site of care, thus potentially improving chlamydia and gonorrhea control efforts.

AB - Tests for Chlamydia trachomatis and Neisseria gonorrhoeae, which can provide results rapidly to guide therapeutic decision-making, offer patient care advantages over laboratory-based tests that require several days to provide results.Wecompared results from the Cepheid GeneXpert CT/NG (Xpert) assay to results from two currently approved nucleic acid amplification assays in 1,722 female and 1,387 male volunteers. Results for chlamydia in females demonstrated sensitivities for endocervical, vaginal, and urine samples of 97.4%, 98.7%, and 97.6%, respectively, and for urine samples from males, a sensitivity of 97.5%, with all specificity estimates being ≥99.4%. Results for gonorrhea in females demonstrated sensitivities for endocervical, vaginal, and urine samples of 100.0%, 100.0%, and 95.6%, respectively, and for urine samples from males, a sensitivity of 98.0%, with all estimates of specificity being ≥99.8%. These results indicate that this short-turnaround-time test can be used to accurately test patients and to possibly do so at the site of care, thus potentially improving chlamydia and gonorrhea control efforts.

UR - http://www.scopus.com/inward/record.url?scp=84878517141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878517141&partnerID=8YFLogxK

U2 - 10.1128/JCM.03461-12

DO - 10.1128/JCM.03461-12

M3 - Article

VL - 51

SP - 1666

EP - 1672

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 6

ER -