Performance of focus ELISA tests for HSV-1 and HSV-2 antibodies among university students with no history of genital herpes

Hayley D. Mark, Joy P. Nanda, Jessica Williams, Anne Rompalo, Johan H. Melendez, Jonathan Zenilman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

OBJECTIVES: To define the performance characteristics of the Focus ELISA HSV-1 and HSV-2 assay among 100 university students. STUDY DESIGN: HSV-1 and HSV-2 Focus ELISA and Western Blot assays were performed on sera from university students who reported no history of genital herpes. RESULTS: HSV-2 and HSV-1 seroprevalence by Western Blot were 3.4% and 48%, respectively. In this population, the positive predictive value of the Focus HSV-2 ELISA was 37.5%, the sensitivity was 100%, and specificity was 94.1%. The PPV of the Focus HSV-1 ELISA was 96.7%, the sensitivity was 69.0%, and the specificity was 97.8%. CONCLUSIONS: In this low-prevalence population, the positive predictive value of the Focus HSV-2 ELISA test was low. This finding, together with those reported elsewhere, indicates that caution is warranted when recommending HSV screening in low-prevalence or heterogeneous populations. Consideration should be given to raising the cutoff index value for defining a positive test result.

Original languageEnglish
Pages (from-to)681-685
Number of pages5
JournalSexually Transmitted Diseases
Volume34
Issue number9
DOIs
StatePublished - Sep 2007
Externally publishedYes

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Herpes Genitalis
Human Herpesvirus 2
Human Herpesvirus 1
Enzyme-Linked Immunosorbent Assay
Students
Antibodies
Western Blotting
Population
Seroepidemiologic Studies
Serum

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)

Cite this

Performance of focus ELISA tests for HSV-1 and HSV-2 antibodies among university students with no history of genital herpes. / Mark, Hayley D.; Nanda, Joy P.; Williams, Jessica; Rompalo, Anne; Melendez, Johan H.; Zenilman, Jonathan.

In: Sexually Transmitted Diseases, Vol. 34, No. 9, 09.2007, p. 681-685.

Research output: Contribution to journalArticle

Mark, Hayley D. ; Nanda, Joy P. ; Williams, Jessica ; Rompalo, Anne ; Melendez, Johan H. ; Zenilman, Jonathan. / Performance of focus ELISA tests for HSV-1 and HSV-2 antibodies among university students with no history of genital herpes. In: Sexually Transmitted Diseases. 2007 ; Vol. 34, No. 9. pp. 681-685.
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