Performance of focus ELISA tests for HSV-1 and HSV-2 antibodies among university students with no history of genital herpes

Hayley D. Mark, Joy P. Nanda, Jessica Roberts, Anne Rompalo, Johan H. Melendez, Jonathan Zenilman

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

OBJECTIVES: To define the performance characteristics of the Focus ELISA HSV-1 and HSV-2 assay among 100 university students. STUDY DESIGN: HSV-1 and HSV-2 Focus ELISA and Western Blot assays were performed on sera from university students who reported no history of genital herpes. RESULTS: HSV-2 and HSV-1 seroprevalence by Western Blot were 3.4% and 48%, respectively. In this population, the positive predictive value of the Focus HSV-2 ELISA was 37.5%, the sensitivity was 100%, and specificity was 94.1%. The PPV of the Focus HSV-1 ELISA was 96.7%, the sensitivity was 69.0%, and the specificity was 97.8%. CONCLUSIONS: In this low-prevalence population, the positive predictive value of the Focus HSV-2 ELISA test was low. This finding, together with those reported elsewhere, indicates that caution is warranted when recommending HSV screening in low-prevalence or heterogeneous populations. Consideration should be given to raising the cutoff index value for defining a positive test result.

Original languageEnglish (US)
Pages (from-to)681-685
Number of pages5
JournalSexually Transmitted Diseases
Volume34
Issue number9
DOIs
StatePublished - Sep 1 2007

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

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