Perforin expression in human cell-mediated luteolysis

A. Hameed, W. M. Fox, R. J. Kurman, R. H. Hruban, E. R. Podack

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

After ovulation and in the absence of fertilization, the human corpus luteum regresses in an orderly sequence of morphological changes. This study demonstrated that luteal regression involved progressive infiltration of lymphocytes and macrophages. The inflammatory infiltrate began in the theca externa and gradually invaded granulosa cells, with maximum accumulation of lymphocytes and macrophages at the time of menstruation. Immunoperoxidase staining showed that the majority of lymphocytes were CD2+, CD3+, CD8+ T lymphocytes, and 15% of these T cells expressed perforin, a cytolytic protein implicated as a mediator of cytotoxicity. The remaining mononuclear infiltrate showed strong reactivity with monocyte/macrophage markers. These findings indicate that (a) a physiologic cell-mediated inflammatory process in the regressing human corpus luteum is mediated mainly by CD8+ T lymphocytes and cells of monocyte/macrophage lineage and (b) perforin expression in T lymphocytes supports a possible role for cytolytic T cells during the physiologic inflammatory response in human luteolysis.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalInternational Journal of Gynecological Pathology
Volume14
Issue number2
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Luteolysis
Perforin
T-Lymphocytes
Macrophages
Corpus Luteum
Lymphocytes
Monocytes
Theca Cells
Menstruation
Granulosa Cells
Ovulation
Fertilization
Staining and Labeling
Proteins

Keywords

  • Corpus luteum
  • Immunohistochemistry
  • Luteal regression
  • Perforin

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Pathology and Forensic Medicine

Cite this

Hameed, A., Fox, W. M., Kurman, R. J., Hruban, R. H., & Podack, E. R. (1995). Perforin expression in human cell-mediated luteolysis. International Journal of Gynecological Pathology, 14(2), 151-157.

Perforin expression in human cell-mediated luteolysis. / Hameed, A.; Fox, W. M.; Kurman, R. J.; Hruban, R. H.; Podack, E. R.

In: International Journal of Gynecological Pathology, Vol. 14, No. 2, 01.01.1995, p. 151-157.

Research output: Contribution to journalArticle

Hameed, A, Fox, WM, Kurman, RJ, Hruban, RH & Podack, ER 1995, 'Perforin expression in human cell-mediated luteolysis', International Journal of Gynecological Pathology, vol. 14, no. 2, pp. 151-157.
Hameed A, Fox WM, Kurman RJ, Hruban RH, Podack ER. Perforin expression in human cell-mediated luteolysis. International Journal of Gynecological Pathology. 1995 Jan 1;14(2):151-157.
Hameed, A. ; Fox, W. M. ; Kurman, R. J. ; Hruban, R. H. ; Podack, E. R. / Perforin expression in human cell-mediated luteolysis. In: International Journal of Gynecological Pathology. 1995 ; Vol. 14, No. 2. pp. 151-157.
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