Perforin expression by thyroid-infiltrating T cells in autoimmune thyroid disease

Z. Wu, E. R. Podack, J. M. McKenzie, K. J. Olsen, M. Zakarija

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Infiltration of the thyroid gland by lymphocytes is a hall-mark of autoimmune thyroid disease; it is particularly evident in Hashimoto's thyroiditis but is also seen in most patients with Graves' disease. Infiltrating cells are comprised primarily of T lymphocytes, of which only a minority appears to be activated. Their precise pathogenic role is largely unknown. Since perforin has been a marker for functionally activated cytotoxic T cells in situ we elected to assess the presence of perforin-containing cells in thyroid-infiltrating lymphocytes and establish their phenotype. Cells were isolated from seven subtotal thyroidectomy specimens, five from patients with Graves' disease and two with Hashimoto's thyroiditis. The novel findings were as follows: CD4+ perforin-containing T cells occurred only in Hashimoto's glands, suggesting a class II-restricted component of cytotoxicity; in Graves' disease, and to a lesser extent in Hashimoto's, perforin-expressing cells were primarily T cell receptor αβ+ CD4-CD8- (double negative); double negative perforin-containing cells in peripheral blood of normal individuals were largely γδ+ T cells. In Hashimoto's samples, the predominant population of T cells expressing perforin was CD8+. By comparison, in studies of the synovial fluid of knee joints from patients with rheumatoid arthritis only a minor population of the perforin-containing cells was double-negative. The data suggest significant differences in cytotoxic autoimmune mechanisms between the two autoimmune thyroid diseases. Functional characterization of double-negative T cells is necessary to define their role in autoimmunity.

Original languageEnglish
Pages (from-to)470-477
Number of pages8
JournalClinical and Experimental Immunology
Volume98
Issue number3
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Perforin
Thyroid Diseases
Autoimmune Diseases
Thyroid Gland
T-Lymphocytes
Graves Disease
Hashimoto Disease
Lymphocytes
Synovial Fluid
Thyroidectomy
Knee Joint
T-Cell Antigen Receptor
Autoimmunity
Population
Rheumatoid Arthritis
Phenotype

Keywords

  • αβ T cell receptor
  • CD4 cytotoxic T cells
  • double-negative T cells
  • perforin
  • thyroid autoimmunity

ASJC Scopus subject areas

  • Immunology

Cite this

Wu, Z., Podack, E. R., McKenzie, J. M., Olsen, K. J., & Zakarija, M. (1994). Perforin expression by thyroid-infiltrating T cells in autoimmune thyroid disease. Clinical and Experimental Immunology, 98(3), 470-477.

Perforin expression by thyroid-infiltrating T cells in autoimmune thyroid disease. / Wu, Z.; Podack, E. R.; McKenzie, J. M.; Olsen, K. J.; Zakarija, M.

In: Clinical and Experimental Immunology, Vol. 98, No. 3, 01.01.1994, p. 470-477.

Research output: Contribution to journalArticle

Wu, Z, Podack, ER, McKenzie, JM, Olsen, KJ & Zakarija, M 1994, 'Perforin expression by thyroid-infiltrating T cells in autoimmune thyroid disease', Clinical and Experimental Immunology, vol. 98, no. 3, pp. 470-477.
Wu Z, Podack ER, McKenzie JM, Olsen KJ, Zakarija M. Perforin expression by thyroid-infiltrating T cells in autoimmune thyroid disease. Clinical and Experimental Immunology. 1994 Jan 1;98(3):470-477.
Wu, Z. ; Podack, E. R. ; McKenzie, J. M. ; Olsen, K. J. ; Zakarija, M. / Perforin expression by thyroid-infiltrating T cells in autoimmune thyroid disease. In: Clinical and Experimental Immunology. 1994 ; Vol. 98, No. 3. pp. 470-477.
@article{3fb946c5f81b4aa7bc7a3479ad550529,
title = "Perforin expression by thyroid-infiltrating T cells in autoimmune thyroid disease",
abstract = "Infiltration of the thyroid gland by lymphocytes is a hall-mark of autoimmune thyroid disease; it is particularly evident in Hashimoto's thyroiditis but is also seen in most patients with Graves' disease. Infiltrating cells are comprised primarily of T lymphocytes, of which only a minority appears to be activated. Their precise pathogenic role is largely unknown. Since perforin has been a marker for functionally activated cytotoxic T cells in situ we elected to assess the presence of perforin-containing cells in thyroid-infiltrating lymphocytes and establish their phenotype. Cells were isolated from seven subtotal thyroidectomy specimens, five from patients with Graves' disease and two with Hashimoto's thyroiditis. The novel findings were as follows: CD4+ perforin-containing T cells occurred only in Hashimoto's glands, suggesting a class II-restricted component of cytotoxicity; in Graves' disease, and to a lesser extent in Hashimoto's, perforin-expressing cells were primarily T cell receptor αβ+ CD4-CD8- (double negative); double negative perforin-containing cells in peripheral blood of normal individuals were largely γδ+ T cells. In Hashimoto's samples, the predominant population of T cells expressing perforin was CD8+. By comparison, in studies of the synovial fluid of knee joints from patients with rheumatoid arthritis only a minor population of the perforin-containing cells was double-negative. The data suggest significant differences in cytotoxic autoimmune mechanisms between the two autoimmune thyroid diseases. Functional characterization of double-negative T cells is necessary to define their role in autoimmunity.",
keywords = "αβ T cell receptor, CD4 cytotoxic T cells, double-negative T cells, perforin, thyroid autoimmunity",
author = "Z. Wu and Podack, {E. R.} and McKenzie, {J. M.} and Olsen, {K. J.} and M. Zakarija",
year = "1994",
month = "1",
day = "1",
language = "English",
volume = "98",
pages = "470--477",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Perforin expression by thyroid-infiltrating T cells in autoimmune thyroid disease

AU - Wu, Z.

AU - Podack, E. R.

AU - McKenzie, J. M.

AU - Olsen, K. J.

AU - Zakarija, M.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Infiltration of the thyroid gland by lymphocytes is a hall-mark of autoimmune thyroid disease; it is particularly evident in Hashimoto's thyroiditis but is also seen in most patients with Graves' disease. Infiltrating cells are comprised primarily of T lymphocytes, of which only a minority appears to be activated. Their precise pathogenic role is largely unknown. Since perforin has been a marker for functionally activated cytotoxic T cells in situ we elected to assess the presence of perforin-containing cells in thyroid-infiltrating lymphocytes and establish their phenotype. Cells were isolated from seven subtotal thyroidectomy specimens, five from patients with Graves' disease and two with Hashimoto's thyroiditis. The novel findings were as follows: CD4+ perforin-containing T cells occurred only in Hashimoto's glands, suggesting a class II-restricted component of cytotoxicity; in Graves' disease, and to a lesser extent in Hashimoto's, perforin-expressing cells were primarily T cell receptor αβ+ CD4-CD8- (double negative); double negative perforin-containing cells in peripheral blood of normal individuals were largely γδ+ T cells. In Hashimoto's samples, the predominant population of T cells expressing perforin was CD8+. By comparison, in studies of the synovial fluid of knee joints from patients with rheumatoid arthritis only a minor population of the perforin-containing cells was double-negative. The data suggest significant differences in cytotoxic autoimmune mechanisms between the two autoimmune thyroid diseases. Functional characterization of double-negative T cells is necessary to define their role in autoimmunity.

AB - Infiltration of the thyroid gland by lymphocytes is a hall-mark of autoimmune thyroid disease; it is particularly evident in Hashimoto's thyroiditis but is also seen in most patients with Graves' disease. Infiltrating cells are comprised primarily of T lymphocytes, of which only a minority appears to be activated. Their precise pathogenic role is largely unknown. Since perforin has been a marker for functionally activated cytotoxic T cells in situ we elected to assess the presence of perforin-containing cells in thyroid-infiltrating lymphocytes and establish their phenotype. Cells were isolated from seven subtotal thyroidectomy specimens, five from patients with Graves' disease and two with Hashimoto's thyroiditis. The novel findings were as follows: CD4+ perforin-containing T cells occurred only in Hashimoto's glands, suggesting a class II-restricted component of cytotoxicity; in Graves' disease, and to a lesser extent in Hashimoto's, perforin-expressing cells were primarily T cell receptor αβ+ CD4-CD8- (double negative); double negative perforin-containing cells in peripheral blood of normal individuals were largely γδ+ T cells. In Hashimoto's samples, the predominant population of T cells expressing perforin was CD8+. By comparison, in studies of the synovial fluid of knee joints from patients with rheumatoid arthritis only a minor population of the perforin-containing cells was double-negative. The data suggest significant differences in cytotoxic autoimmune mechanisms between the two autoimmune thyroid diseases. Functional characterization of double-negative T cells is necessary to define their role in autoimmunity.

KW - αβ T cell receptor

KW - CD4 cytotoxic T cells

KW - double-negative T cells

KW - perforin

KW - thyroid autoimmunity

UR - http://www.scopus.com/inward/record.url?scp=0028114746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028114746&partnerID=8YFLogxK

M3 - Article

C2 - 7994911

AN - SCOPUS:0028114746

VL - 98

SP - 470

EP - 477

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 3

ER -