Perfluorocarbon emulsions improve cognitive recovery after lateral fluid percussion brain injury in rats

Zhengwen Zhou, Dong Sun, Joseph E. Levasseur, Amedeo Merenda, Robert J. Hamm, Jiepei Zhu, Bruce D. Spiess, M. Ross Bullock

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


OBJECTIVE: Perfluorocarbon emulsions have been shown to improve outcomes in stroke models. This study examined the effect of Oxycyte, a third-generation perfluorocarbon emulsion (04RD33; Synthetic Blood International, Inc., Costa Mesa, CA) treatment on cognitive recovery and mitochondrial oxygen consumption after a moderate lateral fluid percussion injury (LFPI). METHODS: Adult male Sprague-Dawley rats (Harlan Bioproducts for Science, Indianapolis, IN) were allocated to 4 groups: 1) LFPI treated with a lower dose of Oxycyte (4.5 mL/kg); 2) LFPI with a higher dose of Oxycyte (9.0 mL/kg); 3) LFPI with saline infusion; and 4) sham animals treated with saline. Fifteen minutes after receiving moderate LFPI or sham surgery, animals were infused intravenously with Oxycyte or saline within 30 minutes while breathing 100% O2. Animals breathed 100% O2 continuously for a total of 4 hours after injury. At 11 to 15 days after LFPI, animals were assessed for cognitive deficits using the Morris water maze test. They were sacrificed at Day 15 after injury for histology to assess hippocampal neuronal cell loss. In a parallel study, mitochondrial oxygen consumption values were measured by the Cartesian diver microrespirometer method. RESULTS: We found that injured animals treated with a lower or higher dose of Oxycyte had significant improvement in cognitive function when compared with injured saline-control animals (P < 0.05). Moreover, injured animals that received either dose of Oxycyte had significantly less neuronal cell loss in the hippocampal CA3 region compared with saline-treated animals (P < 0.05). Furthermore, a lower dose of Oxycyte significantly improved mitochondrial oxygen consumption levels (P < 0.05). CONCLUSION: The current study demonstrates that Oxycyte can improve cognitive recovery and reduce CA3 neuronal cell loss after traumatic brain injury in rats.

Original languageEnglish (US)
Pages (from-to)799-806
Number of pages8
Issue number4
StatePublished - Oct 2008
Externally publishedYes


  • Cognitive deficits
  • Hippocampus
  • Perfluorocarbon emulsion
  • Traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery


Dive into the research topics of 'Perfluorocarbon emulsions improve cognitive recovery after lateral fluid percussion brain injury in rats'. Together they form a unique fingerprint.

Cite this