Perfluorinated alginate for cellular encapsulation

Kerim M. Gattás-Asfura, Christopher Fraker, Cherie L. Stabler

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Molecules of pentadecafluorooctanoyl chloride (PFC) were grafted onto alginate (Alg) using a linear poly(ethylene glycol) linker and amide bonds. The resulting Alg-PFC material was characterized by proton nuclear magnetic resonance and infrared spectroscopies. The degree of PFC functionalization significantly influenced the physical and chemical properties of Alg-PFC, particularly when the resulting polymer was ionically crosslinked into hydrogels. Alg-PFC hydrogel beads fabricated via Ba 2+ crosslinking were found to match the permeability properties of control alginate beads, except upon swelling over time in culture media. When used to encapsulate MIN6 cells, a beta cell line, Alg-PFC beads demonstrated enhanced cell proliferation over alginate control beads. These results indicate that Alg-PFC hydrogels retain some of the PFC's biological-relevant benefits, such as enhancement of mass transport and bioinertness, to enhance cellular viability within alginate three-dimensional hydrogel environments. We envision these functionalized hydrogels to be particularly useful in the encapsulation of cells with a high metabolic demand, such as pancreatic islets.

Original languageEnglish
Pages (from-to)1963-1971
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume100 A
Issue number8
DOIs
StatePublished - Aug 1 2012

Fingerprint

Alginate
Encapsulation
Chlorides
Hydrogels
Hydrogel
alginic acid
Cell proliferation
Amides
Crosslinking
Chemical properties
Nuclear magnetic resonance spectroscopy
Polyethylene glycols
Swelling
Culture Media
Infrared spectroscopy
Polymers
Mass transfer
Physical properties
Cells
Nuclear magnetic resonance

Keywords

  • alginate
  • cellular encapsulation
  • oxygen
  • perfluorocarbon

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Perfluorinated alginate for cellular encapsulation. / Gattás-Asfura, Kerim M.; Fraker, Christopher; Stabler, Cherie L.

In: Journal of Biomedical Materials Research - Part A, Vol. 100 A, No. 8, 01.08.2012, p. 1963-1971.

Research output: Contribution to journalArticle

Gattás-Asfura, Kerim M. ; Fraker, Christopher ; Stabler, Cherie L. / Perfluorinated alginate for cellular encapsulation. In: Journal of Biomedical Materials Research - Part A. 2012 ; Vol. 100 A, No. 8. pp. 1963-1971.
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