Perception of differentiation cues by GATA factors in primitive endoderm lineage determination of mouse embryonic stem cells

Callinice D. Capo-Chichi, Malgorzata E. Rula, Jennifer L. Smedberg, Lisa Vanderveer, Michael S. Parmacek, Edward E. Morrisey, Andrew K. Godwin, Xiangxi Xu

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The formation of the primitive endoderm covering the inner cell mass of early mouse embryos can be simulated in vitro by the differentiation of mouse embryonic stem (ES) cells in culture following either aggregation of suspended cells or stimulation of cell monolayers with retinoic acid. The developmentally regulated transcription factors GATA-4 and GATA-6 have determining role in mouse extraembryonic endoderm development. We analyzed the in vitro differentiation of mouse embryonic stem cells deficient of GATA factors and conclude that GATA-4 is required for ES cells to perceive a cell positioning (cell aggregation) signal and GATA-6 is required to sense morphogenic (retinoic acid) signal. The collaboration between GATA-6 and GATA-4, or GATA-6 and GATA-5 which can substitute for GATA-4, is involved in the perception of differentiation cues by embryonic stem cells in their determination of endoderm lineage. This study indicates that the lineage differentiation of ES cells can be manipulated by the expression of GATA factors.

Original languageEnglish
Pages (from-to)574-586
Number of pages13
JournalDevelopmental Biology
Volume286
Issue number2
DOIs
StatePublished - Oct 15 2005
Externally publishedYes

Fingerprint

GATA Transcription Factors
Endoderm
Embryonic Stem Cells
Cues
Cell Aggregation
Tretinoin
GATA4 Transcription Factor
Embryonic Structures
Cell Culture Techniques
Mouse Embryonic Stem Cells
In Vitro Techniques

Keywords

  • Cell growth suppression
  • Differentiation
  • Disabled-2
  • GATA factors
  • Primitive endoderm
  • Retinoic acid

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Perception of differentiation cues by GATA factors in primitive endoderm lineage determination of mouse embryonic stem cells. / Capo-Chichi, Callinice D.; Rula, Malgorzata E.; Smedberg, Jennifer L.; Vanderveer, Lisa; Parmacek, Michael S.; Morrisey, Edward E.; Godwin, Andrew K.; Xu, Xiangxi.

In: Developmental Biology, Vol. 286, No. 2, 15.10.2005, p. 574-586.

Research output: Contribution to journalArticle

Capo-Chichi, CD, Rula, ME, Smedberg, JL, Vanderveer, L, Parmacek, MS, Morrisey, EE, Godwin, AK & Xu, X 2005, 'Perception of differentiation cues by GATA factors in primitive endoderm lineage determination of mouse embryonic stem cells', Developmental Biology, vol. 286, no. 2, pp. 574-586. https://doi.org/10.1016/j.ydbio.2005.07.037
Capo-Chichi, Callinice D. ; Rula, Malgorzata E. ; Smedberg, Jennifer L. ; Vanderveer, Lisa ; Parmacek, Michael S. ; Morrisey, Edward E. ; Godwin, Andrew K. ; Xu, Xiangxi. / Perception of differentiation cues by GATA factors in primitive endoderm lineage determination of mouse embryonic stem cells. In: Developmental Biology. 2005 ; Vol. 286, No. 2. pp. 574-586.
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