Perception of differentiation cues by GATA factors in primitive endoderm lineage determination of mouse embryonic stem cells

Callinice D. Capo-Chichi, Malgorzata E. Rula, Jennifer L. Smedberg, Lisa Vanderveer, Michael S. Parmacek, Edward E. Morrisey, Andrew K. Godwin, Xiang Xi Xu

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

The formation of the primitive endoderm covering the inner cell mass of early mouse embryos can be simulated in vitro by the differentiation of mouse embryonic stem (ES) cells in culture following either aggregation of suspended cells or stimulation of cell monolayers with retinoic acid. The developmentally regulated transcription factors GATA-4 and GATA-6 have determining role in mouse extraembryonic endoderm development. We analyzed the in vitro differentiation of mouse embryonic stem cells deficient of GATA factors and conclude that GATA-4 is required for ES cells to perceive a cell positioning (cell aggregation) signal and GATA-6 is required to sense morphogenic (retinoic acid) signal. The collaboration between GATA-6 and GATA-4, or GATA-6 and GATA-5 which can substitute for GATA-4, is involved in the perception of differentiation cues by embryonic stem cells in their determination of endoderm lineage. This study indicates that the lineage differentiation of ES cells can be manipulated by the expression of GATA factors.

Original languageEnglish (US)
Pages (from-to)574-586
Number of pages13
JournalDevelopmental Biology
Volume286
Issue number2
DOIs
StatePublished - Oct 15 2005
Externally publishedYes

Keywords

  • Cell growth suppression
  • Differentiation
  • Disabled-2
  • GATA factors
  • Primitive endoderm
  • Retinoic acid

ASJC Scopus subject areas

  • Developmental Biology

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    Capo-Chichi, C. D., Rula, M. E., Smedberg, J. L., Vanderveer, L., Parmacek, M. S., Morrisey, E. E., Godwin, A. K., & Xu, X. X. (2005). Perception of differentiation cues by GATA factors in primitive endoderm lineage determination of mouse embryonic stem cells. Developmental Biology, 286(2), 574-586. https://doi.org/10.1016/j.ydbio.2005.07.037