Pentobarbital-activated Cl- channels in cultured adult and embryonic human DRG neurons

Alexander Y. Valeyev, John C. Hackman, Alice M. Holohean, Patrick M. Wood, Robert A. Davidoff

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Developmental differences in pentobarbital-activated Cl- currents were studied in adult and embryonic human dorsal root ganglia (DRG) neurons using whole-cell patch-clamp recordings. Pentobarbital-induced Cl- conductance was significantly greater in adult DRGs (28.4 pS) than in embryonic cells (19.1 pS). Fluctuation analysis of the spectral density plots of Cl- channel activation by pentobarbital showed age differences in the length and number of open time constants (adult cells, τ1, τ2, τ3 were 224, 8.4, 1.5 ms, respectively; embryonic cells τ1 and τ2 were 165 and 26.3 ms, respectively). The different kinetic properties of human adult and embryonic DRG Cl- channels opened by pentobarbital may reflect the presence of different subunits in the two populations of neurons. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalDevelopmental Brain Research
Volume124
Issue number1-2
DOIs
StatePublished - Nov 30 2000

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Keywords

  • Cl channel
  • Conductance
  • Dorsal root ganglion
  • Embryonic and adult human neurons
  • Fluctuation analysis
  • GABA receptor
  • Patch clamp
  • Pentobarbital

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Valeyev, A. Y., Hackman, J. C., Holohean, A. M., Wood, P. M., & Davidoff, R. A. (2000). Pentobarbital-activated Cl- channels in cultured adult and embryonic human DRG neurons. Developmental Brain Research, 124(1-2), 137-140. https://doi.org/10.1016/S0165-3806(00)00117-6