PEMF fails to enhance nerve regeneration after sciatic nerve crush lesion

Abrahão Fontes Baptista, Bruno Teixeira Goes, Diego Menezes, Flávia Carvalho Alcantara Gomes, João Zugaib, Joice Stipursky, Joyce Gomes-Osman, Júlia Teixeira Oliveira, Marcos André Vannier-Santos, Ana Maria Blanco Martinez

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The use of electromagnetic fields has been reported to enhance peripheral nerve regeneration. This study aimed to identify the effects of a prolonged protocol of low-frequency pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Thirty-four male Swiss mice (Mus musculus) were divided into PEMF (n = 17) and control (n = 17) groups. All animals underwent a unilateral sciatic-crush lesion, and the PEMF group was exposed to a 72-Hz, 2-G electromagnetic field for 30 min, five days a week, for three weeks. Functional analysis was carried out weekly. After three weeks, the animals were euthanized, and histological, morphometric, oxidative stress, and TGF-β1 analyses were performed. Functional analysis showed no differences between the groups. Histological appearance was similar between PEMF and control nerves. Morphometric assessment showed that the PEMF nerves trended toward decreased regeneration. The levels of free radicals were more pronounced in PEMF nerves, but were not associated with an increase in the content of the TGF-β1/Smad signaling pathway. Prolonged PEMF regimen leads to delayed histological peripheral nerve regeneration and increased oxidative stress but no loss of function recovery.

Original languageEnglish (US)
Pages (from-to)285-293
Number of pages9
JournalJournal of the Peripheral Nervous System
Volume14
Issue number4
DOIs
StatePublished - Dec 2009
Externally publishedYes

Fingerprint

Nerve Crush
Nerve Regeneration
Electromagnetic Fields
Sciatic Nerve
Peripheral Nerves
Oxidative Stress
Recovery of Function
Free Radicals
Regeneration

Keywords

  • Electromagnetic fields
  • Mouse
  • Regeneration
  • Sciatic nerve lesion

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Baptista, A. F., Goes, B. T., Menezes, D., Gomes, F. C. A., Zugaib, J., Stipursky, J., ... Martinez, A. M. B. (2009). PEMF fails to enhance nerve regeneration after sciatic nerve crush lesion. Journal of the Peripheral Nervous System, 14(4), 285-293. https://doi.org/10.1111/j.1529-8027.2009.00240.x

PEMF fails to enhance nerve regeneration after sciatic nerve crush lesion. / Baptista, Abrahão Fontes; Goes, Bruno Teixeira; Menezes, Diego; Gomes, Flávia Carvalho Alcantara; Zugaib, João; Stipursky, Joice; Gomes-Osman, Joyce; Oliveira, Júlia Teixeira; Vannier-Santos, Marcos André; Martinez, Ana Maria Blanco.

In: Journal of the Peripheral Nervous System, Vol. 14, No. 4, 12.2009, p. 285-293.

Research output: Contribution to journalArticle

Baptista, AF, Goes, BT, Menezes, D, Gomes, FCA, Zugaib, J, Stipursky, J, Gomes-Osman, J, Oliveira, JT, Vannier-Santos, MA & Martinez, AMB 2009, 'PEMF fails to enhance nerve regeneration after sciatic nerve crush lesion', Journal of the Peripheral Nervous System, vol. 14, no. 4, pp. 285-293. https://doi.org/10.1111/j.1529-8027.2009.00240.x
Baptista, Abrahão Fontes ; Goes, Bruno Teixeira ; Menezes, Diego ; Gomes, Flávia Carvalho Alcantara ; Zugaib, João ; Stipursky, Joice ; Gomes-Osman, Joyce ; Oliveira, Júlia Teixeira ; Vannier-Santos, Marcos André ; Martinez, Ana Maria Blanco. / PEMF fails to enhance nerve regeneration after sciatic nerve crush lesion. In: Journal of the Peripheral Nervous System. 2009 ; Vol. 14, No. 4. pp. 285-293.
@article{47f0659c04be42a4a6c19fd26f04cfd0,
title = "PEMF fails to enhance nerve regeneration after sciatic nerve crush lesion",
abstract = "The use of electromagnetic fields has been reported to enhance peripheral nerve regeneration. This study aimed to identify the effects of a prolonged protocol of low-frequency pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Thirty-four male Swiss mice (Mus musculus) were divided into PEMF (n = 17) and control (n = 17) groups. All animals underwent a unilateral sciatic-crush lesion, and the PEMF group was exposed to a 72-Hz, 2-G electromagnetic field for 30 min, five days a week, for three weeks. Functional analysis was carried out weekly. After three weeks, the animals were euthanized, and histological, morphometric, oxidative stress, and TGF-β1 analyses were performed. Functional analysis showed no differences between the groups. Histological appearance was similar between PEMF and control nerves. Morphometric assessment showed that the PEMF nerves trended toward decreased regeneration. The levels of free radicals were more pronounced in PEMF nerves, but were not associated with an increase in the content of the TGF-β1/Smad signaling pathway. Prolonged PEMF regimen leads to delayed histological peripheral nerve regeneration and increased oxidative stress but no loss of function recovery.",
keywords = "Electromagnetic fields, Mouse, Regeneration, Sciatic nerve lesion",
author = "Baptista, {Abrah{\~a}o Fontes} and Goes, {Bruno Teixeira} and Diego Menezes and Gomes, {Fl{\'a}via Carvalho Alcantara} and Jo{\~a}o Zugaib and Joice Stipursky and Joyce Gomes-Osman and Oliveira, {J{\'u}lia Teixeira} and Vannier-Santos, {Marcos Andr{\'e}} and Martinez, {Ana Maria Blanco}",
year = "2009",
month = "12",
doi = "10.1111/j.1529-8027.2009.00240.x",
language = "English (US)",
volume = "14",
pages = "285--293",
journal = "Journal of the Peripheral Nervous System",
issn = "1085-9489",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - PEMF fails to enhance nerve regeneration after sciatic nerve crush lesion

AU - Baptista, Abrahão Fontes

AU - Goes, Bruno Teixeira

AU - Menezes, Diego

AU - Gomes, Flávia Carvalho Alcantara

AU - Zugaib, João

AU - Stipursky, Joice

AU - Gomes-Osman, Joyce

AU - Oliveira, Júlia Teixeira

AU - Vannier-Santos, Marcos André

AU - Martinez, Ana Maria Blanco

PY - 2009/12

Y1 - 2009/12

N2 - The use of electromagnetic fields has been reported to enhance peripheral nerve regeneration. This study aimed to identify the effects of a prolonged protocol of low-frequency pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Thirty-four male Swiss mice (Mus musculus) were divided into PEMF (n = 17) and control (n = 17) groups. All animals underwent a unilateral sciatic-crush lesion, and the PEMF group was exposed to a 72-Hz, 2-G electromagnetic field for 30 min, five days a week, for three weeks. Functional analysis was carried out weekly. After three weeks, the animals were euthanized, and histological, morphometric, oxidative stress, and TGF-β1 analyses were performed. Functional analysis showed no differences between the groups. Histological appearance was similar between PEMF and control nerves. Morphometric assessment showed that the PEMF nerves trended toward decreased regeneration. The levels of free radicals were more pronounced in PEMF nerves, but were not associated with an increase in the content of the TGF-β1/Smad signaling pathway. Prolonged PEMF regimen leads to delayed histological peripheral nerve regeneration and increased oxidative stress but no loss of function recovery.

AB - The use of electromagnetic fields has been reported to enhance peripheral nerve regeneration. This study aimed to identify the effects of a prolonged protocol of low-frequency pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Thirty-four male Swiss mice (Mus musculus) were divided into PEMF (n = 17) and control (n = 17) groups. All animals underwent a unilateral sciatic-crush lesion, and the PEMF group was exposed to a 72-Hz, 2-G electromagnetic field for 30 min, five days a week, for three weeks. Functional analysis was carried out weekly. After three weeks, the animals were euthanized, and histological, morphometric, oxidative stress, and TGF-β1 analyses were performed. Functional analysis showed no differences between the groups. Histological appearance was similar between PEMF and control nerves. Morphometric assessment showed that the PEMF nerves trended toward decreased regeneration. The levels of free radicals were more pronounced in PEMF nerves, but were not associated with an increase in the content of the TGF-β1/Smad signaling pathway. Prolonged PEMF regimen leads to delayed histological peripheral nerve regeneration and increased oxidative stress but no loss of function recovery.

KW - Electromagnetic fields

KW - Mouse

KW - Regeneration

KW - Sciatic nerve lesion

UR - http://www.scopus.com/inward/record.url?scp=73549113284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73549113284&partnerID=8YFLogxK

U2 - 10.1111/j.1529-8027.2009.00240.x

DO - 10.1111/j.1529-8027.2009.00240.x

M3 - Article

C2 - 20021570

AN - SCOPUS:73549113284

VL - 14

SP - 285

EP - 293

JO - Journal of the Peripheral Nervous System

JF - Journal of the Peripheral Nervous System

SN - 1085-9489

IS - 4

ER -