Abstract
The use of electromagnetic fields has been reported to enhance peripheral nerve regeneration. This study aimed to identify the effects of a prolonged protocol of low-frequency pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Thirty-four male Swiss mice (Mus musculus) were divided into PEMF (n = 17) and control (n = 17) groups. All animals underwent a unilateral sciatic-crush lesion, and the PEMF group was exposed to a 72-Hz, 2-G electromagnetic field for 30 min, five days a week, for three weeks. Functional analysis was carried out weekly. After three weeks, the animals were euthanized, and histological, morphometric, oxidative stress, and TGF-β1 analyses were performed. Functional analysis showed no differences between the groups. Histological appearance was similar between PEMF and control nerves. Morphometric assessment showed that the PEMF nerves trended toward decreased regeneration. The levels of free radicals were more pronounced in PEMF nerves, but were not associated with an increase in the content of the TGF-β1/Smad signaling pathway. Prolonged PEMF regimen leads to delayed histological peripheral nerve regeneration and increased oxidative stress but no loss of function recovery.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 285-293 |
| Number of pages | 9 |
| Journal | Journal of the Peripheral Nervous System |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 2009 |
| Externally published | Yes |
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Keywords
- Electromagnetic fields
- Mouse
- Regeneration
- Sciatic nerve lesion
ASJC Scopus subject areas
- Clinical Neurology
- Neuroscience(all)
Cite this
PEMF fails to enhance nerve regeneration after sciatic nerve crush lesion. / Baptista, Abrahão Fontes; Goes, Bruno Teixeira; Menezes, Diego; Gomes, Flávia Carvalho Alcantara; Zugaib, João; Stipursky, Joice; Gomes-Osman, Joyce; Oliveira, Júlia Teixeira; Vannier-Santos, Marcos André; Martinez, Ana Maria Blanco.
In: Journal of the Peripheral Nervous System, Vol. 14, No. 4, 12.2009, p. 285-293.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - PEMF fails to enhance nerve regeneration after sciatic nerve crush lesion
AU - Baptista, Abrahão Fontes
AU - Goes, Bruno Teixeira
AU - Menezes, Diego
AU - Gomes, Flávia Carvalho Alcantara
AU - Zugaib, João
AU - Stipursky, Joice
AU - Gomes-Osman, Joyce
AU - Oliveira, Júlia Teixeira
AU - Vannier-Santos, Marcos André
AU - Martinez, Ana Maria Blanco
PY - 2009/12
Y1 - 2009/12
N2 - The use of electromagnetic fields has been reported to enhance peripheral nerve regeneration. This study aimed to identify the effects of a prolonged protocol of low-frequency pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Thirty-four male Swiss mice (Mus musculus) were divided into PEMF (n = 17) and control (n = 17) groups. All animals underwent a unilateral sciatic-crush lesion, and the PEMF group was exposed to a 72-Hz, 2-G electromagnetic field for 30 min, five days a week, for three weeks. Functional analysis was carried out weekly. After three weeks, the animals were euthanized, and histological, morphometric, oxidative stress, and TGF-β1 analyses were performed. Functional analysis showed no differences between the groups. Histological appearance was similar between PEMF and control nerves. Morphometric assessment showed that the PEMF nerves trended toward decreased regeneration. The levels of free radicals were more pronounced in PEMF nerves, but were not associated with an increase in the content of the TGF-β1/Smad signaling pathway. Prolonged PEMF regimen leads to delayed histological peripheral nerve regeneration and increased oxidative stress but no loss of function recovery.
AB - The use of electromagnetic fields has been reported to enhance peripheral nerve regeneration. This study aimed to identify the effects of a prolonged protocol of low-frequency pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Thirty-four male Swiss mice (Mus musculus) were divided into PEMF (n = 17) and control (n = 17) groups. All animals underwent a unilateral sciatic-crush lesion, and the PEMF group was exposed to a 72-Hz, 2-G electromagnetic field for 30 min, five days a week, for three weeks. Functional analysis was carried out weekly. After three weeks, the animals were euthanized, and histological, morphometric, oxidative stress, and TGF-β1 analyses were performed. Functional analysis showed no differences between the groups. Histological appearance was similar between PEMF and control nerves. Morphometric assessment showed that the PEMF nerves trended toward decreased regeneration. The levels of free radicals were more pronounced in PEMF nerves, but were not associated with an increase in the content of the TGF-β1/Smad signaling pathway. Prolonged PEMF regimen leads to delayed histological peripheral nerve regeneration and increased oxidative stress but no loss of function recovery.
KW - Electromagnetic fields
KW - Mouse
KW - Regeneration
KW - Sciatic nerve lesion
UR - http://www.scopus.com/inward/record.url?scp=73549113284&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=73549113284&partnerID=8YFLogxK
U2 - 10.1111/j.1529-8027.2009.00240.x
DO - 10.1111/j.1529-8027.2009.00240.x
M3 - Article
C2 - 20021570
AN - SCOPUS:73549113284
VL - 14
SP - 285
EP - 293
JO - Journal of the Peripheral Nervous System
JF - Journal of the Peripheral Nervous System
SN - 1085-9489
IS - 4
ER -