PEA3 transactivates the Muc4/sialomucin complex promoter in mammary epithelial and tumor cells

Aymee Perez, Roy Barco, Isabel Fernandez, Shari A. Price-Schiavi, Kermit L. Carraway

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Sialomucin complex (SMC, rat Muc4) is a heterodimeric glycoprotein composed of two subunits, the mucin component ascites sialoglycoprotein ASGP-1 and the transmembrane subunit ASGP-2, which is aberrantly expressed on the surfaces of a variety of tumor cells. Up-regulation of the Muc4/SMC gene in the 13762 sublines of the rat mammary adenocarcinoma correlates with the overexpression of transcription factor PEA3 and the receptor tyrosine kinase ErbB2. Here we report that PEA3 is capable of transactivating the Muc4/SMC promoter in a dose-dependent manner via direct attachment to a PEA3 binding site. ERM and ER81, the other two members of the PEA3 subfamily of transcription factors, could not transactivate the Muc4/SMC promoter. Transcriptional activation of Muc4/SMC by PEA3 is potentiated by Ras and MEKK1 kinases. These data suggest that expression of PEA3 in mammary tumors leads to up-regulation of Muc4/SMC transcription, the gene product of which may contribute to the metastatic potential of mammary tumors.

Original languageEnglish
Pages (from-to)36942-36952
Number of pages11
JournalJournal of Biological Chemistry
Volume278
Issue number38
DOIs
StatePublished - Sep 19 2003

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Mucin-4
Sialomucins
Tumors
Epithelial Cells
Cells
Breast Neoplasms
Rats
Up-Regulation
Genes
Sialoglycoproteins
Receptor Protein-Tyrosine Kinases
Mucins
Transcription
Transcriptional Activation
Glycoproteins
Adenocarcinoma
Breast
Transcription Factors
Phosphotransferases
Chemical activation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Perez, A., Barco, R., Fernandez, I., Price-Schiavi, S. A., & Carraway, K. L. (2003). PEA3 transactivates the Muc4/sialomucin complex promoter in mammary epithelial and tumor cells. Journal of Biological Chemistry, 278(38), 36942-36952. https://doi.org/10.1074/jbc.M300264200

PEA3 transactivates the Muc4/sialomucin complex promoter in mammary epithelial and tumor cells. / Perez, Aymee; Barco, Roy; Fernandez, Isabel; Price-Schiavi, Shari A.; Carraway, Kermit L.

In: Journal of Biological Chemistry, Vol. 278, No. 38, 19.09.2003, p. 36942-36952.

Research output: Contribution to journalArticle

Perez, A, Barco, R, Fernandez, I, Price-Schiavi, SA & Carraway, KL 2003, 'PEA3 transactivates the Muc4/sialomucin complex promoter in mammary epithelial and tumor cells', Journal of Biological Chemistry, vol. 278, no. 38, pp. 36942-36952. https://doi.org/10.1074/jbc.M300264200
Perez, Aymee ; Barco, Roy ; Fernandez, Isabel ; Price-Schiavi, Shari A. ; Carraway, Kermit L. / PEA3 transactivates the Muc4/sialomucin complex promoter in mammary epithelial and tumor cells. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 38. pp. 36942-36952.
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