Abstract
Sialomucin complex (SMC, rat Muc4) is a heterodimeric glycoprotein composed of two subunits, the mucin component ascites sialoglycoprotein ASGP-1 and the transmembrane subunit ASGP-2, which is aberrantly expressed on the surfaces of a variety of tumor cells. Up-regulation of the Muc4/SMC gene in the 13762 sublines of the rat mammary adenocarcinoma correlates with the overexpression of transcription factor PEA3 and the receptor tyrosine kinase ErbB2. Here we report that PEA3 is capable of transactivating the Muc4/SMC promoter in a dose-dependent manner via direct attachment to a PEA3 binding site. ERM and ER81, the other two members of the PEA3 subfamily of transcription factors, could not transactivate the Muc4/SMC promoter. Transcriptional activation of Muc4/SMC by PEA3 is potentiated by Ras and MEKK1 kinases. These data suggest that expression of PEA3 in mammary tumors leads to up-regulation of Muc4/SMC transcription, the gene product of which may contribute to the metastatic potential of mammary tumors.
| Original language | English |
|---|---|
| Pages (from-to) | 36942-36952 |
| Number of pages | 11 |
| Journal | Journal of Biological Chemistry |
| Volume | 278 |
| Issue number | 38 |
| DOIs | |
| State | Published - Sep 19 2003 |
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ASJC Scopus subject areas
- Biochemistry
Cite this
PEA3 transactivates the Muc4/sialomucin complex promoter in mammary epithelial and tumor cells. / Perez, Aymee; Barco, Roy; Fernandez, Isabel; Price-Schiavi, Shari A.; Carraway, Kermit L.
In: Journal of Biological Chemistry, Vol. 278, No. 38, 19.09.2003, p. 36942-36952.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - PEA3 transactivates the Muc4/sialomucin complex promoter in mammary epithelial and tumor cells
AU - Perez, Aymee
AU - Barco, Roy
AU - Fernandez, Isabel
AU - Price-Schiavi, Shari A.
AU - Carraway, Kermit L.
PY - 2003/9/19
Y1 - 2003/9/19
N2 - Sialomucin complex (SMC, rat Muc4) is a heterodimeric glycoprotein composed of two subunits, the mucin component ascites sialoglycoprotein ASGP-1 and the transmembrane subunit ASGP-2, which is aberrantly expressed on the surfaces of a variety of tumor cells. Up-regulation of the Muc4/SMC gene in the 13762 sublines of the rat mammary adenocarcinoma correlates with the overexpression of transcription factor PEA3 and the receptor tyrosine kinase ErbB2. Here we report that PEA3 is capable of transactivating the Muc4/SMC promoter in a dose-dependent manner via direct attachment to a PEA3 binding site. ERM and ER81, the other two members of the PEA3 subfamily of transcription factors, could not transactivate the Muc4/SMC promoter. Transcriptional activation of Muc4/SMC by PEA3 is potentiated by Ras and MEKK1 kinases. These data suggest that expression of PEA3 in mammary tumors leads to up-regulation of Muc4/SMC transcription, the gene product of which may contribute to the metastatic potential of mammary tumors.
AB - Sialomucin complex (SMC, rat Muc4) is a heterodimeric glycoprotein composed of two subunits, the mucin component ascites sialoglycoprotein ASGP-1 and the transmembrane subunit ASGP-2, which is aberrantly expressed on the surfaces of a variety of tumor cells. Up-regulation of the Muc4/SMC gene in the 13762 sublines of the rat mammary adenocarcinoma correlates with the overexpression of transcription factor PEA3 and the receptor tyrosine kinase ErbB2. Here we report that PEA3 is capable of transactivating the Muc4/SMC promoter in a dose-dependent manner via direct attachment to a PEA3 binding site. ERM and ER81, the other two members of the PEA3 subfamily of transcription factors, could not transactivate the Muc4/SMC promoter. Transcriptional activation of Muc4/SMC by PEA3 is potentiated by Ras and MEKK1 kinases. These data suggest that expression of PEA3 in mammary tumors leads to up-regulation of Muc4/SMC transcription, the gene product of which may contribute to the metastatic potential of mammary tumors.
UR - http://www.scopus.com/inward/record.url?scp=0141480192&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141480192&partnerID=8YFLogxK
U2 - 10.1074/jbc.M300264200
DO - 10.1074/jbc.M300264200
M3 - Article
C2 - 12855694
AN - SCOPUS:0141480192
VL - 278
SP - 36942
EP - 36952
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 38
ER -