PDZD7-MYO7A complex identified in enriched stereocilia membranes

Clive P. Morgan, Jocelyn F. Krey, M’hamed Grati, Bo Zhao, Shannon Fallen, Abhiraami Kannan-Sundhari, Xue Z Liu, Dongseok Choi, Ulrich Müller, Peter G. Barr-Gillespie

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

While more than 70 genes have been linked to deafness, most of which are expressed in mechanosensory hair cells of the inner ear, a challenge has been to link these genes into molecular pathways. One example is Myo7a (myosin VIIA), in which deafness mutations affect the development and function of the mechanically sensitive stereocilia of hair cells. We describe here a procedure for the isolation of low-abundance protein complexes from stereocilia membrane fractions. Using this procedure, combined with identification and quantitation of proteins with mass spectrometry, we demonstrate that MYO7A forms a complex with PDZD7, a paralog of USH1C and DFNB31. MYO7A and PDZD7 interact in tissue-culture cells, and co-localize to the ankle-link region of stereocilia in wild-type but not Myo7a mutant mice. Our data thus describe a new paradigm for the interrogation of low-abundance protein complexes in hair cell stereocilia and establish an unanticipated link between MYO7A and PDZD7.

Original languageEnglish (US)
Article numbere18312
JournaleLife
Volume5
Issue numberAUGUST
DOIs
StatePublished - Aug 15 2016

Fingerprint

Stereocilia
Cells
Membranes
Deafness
Genes
Tissue culture
Proteins
Myosins
Mass spectrometry
Inner Ear
Ankle
Mass Spectrometry
Cell Culture Techniques
Mutation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Morgan, C. P., Krey, J. F., Grati, M., Zhao, B., Fallen, S., Kannan-Sundhari, A., ... Barr-Gillespie, P. G. (2016). PDZD7-MYO7A complex identified in enriched stereocilia membranes. eLife, 5(AUGUST), [e18312]. https://doi.org/10.7554/eLife.18312

PDZD7-MYO7A complex identified in enriched stereocilia membranes. / Morgan, Clive P.; Krey, Jocelyn F.; Grati, M’hamed; Zhao, Bo; Fallen, Shannon; Kannan-Sundhari, Abhiraami; Liu, Xue Z; Choi, Dongseok; Müller, Ulrich; Barr-Gillespie, Peter G.

In: eLife, Vol. 5, No. AUGUST, e18312, 15.08.2016.

Research output: Contribution to journalArticle

Morgan, CP, Krey, JF, Grati, M, Zhao, B, Fallen, S, Kannan-Sundhari, A, Liu, XZ, Choi, D, Müller, U & Barr-Gillespie, PG 2016, 'PDZD7-MYO7A complex identified in enriched stereocilia membranes', eLife, vol. 5, no. AUGUST, e18312. https://doi.org/10.7554/eLife.18312
Morgan CP, Krey JF, Grati M, Zhao B, Fallen S, Kannan-Sundhari A et al. PDZD7-MYO7A complex identified in enriched stereocilia membranes. eLife. 2016 Aug 15;5(AUGUST). e18312. https://doi.org/10.7554/eLife.18312
Morgan, Clive P. ; Krey, Jocelyn F. ; Grati, M’hamed ; Zhao, Bo ; Fallen, Shannon ; Kannan-Sundhari, Abhiraami ; Liu, Xue Z ; Choi, Dongseok ; Müller, Ulrich ; Barr-Gillespie, Peter G. / PDZD7-MYO7A complex identified in enriched stereocilia membranes. In: eLife. 2016 ; Vol. 5, No. AUGUST.
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