Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2

C. Forristall, M. Pondel, L. Chen, M. L. King

Research output: Contribution to journalArticle

174 Scopus citations

Abstract

In Xenopus, localization of a rare class of mRNAs during oogenesis is believed to initiate pattern formation in the early embryo. We have determined the pattern of RNA localization for one of these RNAs. Xcat-2, which encodes a putative RNA-binding protein related to Drosophila nanos. Xcat-2 is exclusively localized to the mitochondrial cloud in stage I oocytes. moves with this body into the vegetal cortex during stage II and, later, partitions into islands consistent with it being a component of the germ plasm. As previously shown, Vg1 is not localized to the vegetal cortex until stage TV and distributes to all vegetal blastomeres during development. We found a direct correlation between the localized condition of these RNAs and their recovery in a detergent-insoluble fraction. We present evidence suggesting that differential RNA binding to a cytoskeletal component(s) in the vegetal cortex determines the pattern of inheritance for that RNA in the embryo.

Original languageEnglish (US)
Pages (from-to)201-208
Number of pages8
JournalDevelopment
Volume121
Issue number1
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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    Forristall, C., Pondel, M., Chen, L., & King, M. L. (1995). Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2. Development, 121(1), 201-208.