Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2

C. Forristall, M. Pondel, L. Chen, Mary Lou L King

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

In Xenopus, localization of a rare class of mRNAs during oogenesis is believed to initiate pattern formation in the early embryo. We have determined the pattern of RNA localization for one of these RNAs. Xcat-2, which encodes a putative RNA-binding protein related to Drosophila nanos. Xcat-2 is exclusively localized to the mitochondrial cloud in stage I oocytes. moves with this body into the vegetal cortex during stage II and, later, partitions into islands consistent with it being a component of the germ plasm. As previously shown, Vg1 is not localized to the vegetal cortex until stage TV and distributes to all vegetal blastomeres during development. We found a direct correlation between the localized condition of these RNAs and their recovery in a detergent-insoluble fraction. We present evidence suggesting that differential RNA binding to a cytoskeletal component(s) in the vegetal cortex determines the pattern of inheritance for that RNA in the embryo.

Original languageEnglish
Pages (from-to)201-208
Number of pages8
JournalDevelopment
Volume121
Issue number1
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

RNA
Embryonic Structures
Blastomeres
Inheritance Patterns
Oogenesis
RNA-Binding Proteins
Xenopus
Islands
Detergents
Drosophila
Oocytes
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Forristall, C., Pondel, M., Chen, L., & King, M. L. L. (1995). Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2. Development, 121(1), 201-208.

Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2. / Forristall, C.; Pondel, M.; Chen, L.; King, Mary Lou L.

In: Development, Vol. 121, No. 1, 01.01.1995, p. 201-208.

Research output: Contribution to journalArticle

Forristall, C, Pondel, M, Chen, L & King, MLL 1995, 'Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2', Development, vol. 121, no. 1, pp. 201-208.
Forristall, C. ; Pondel, M. ; Chen, L. ; King, Mary Lou L. / Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2. In: Development. 1995 ; Vol. 121, No. 1. pp. 201-208.
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