Patterns of hairless (hr) gene expression in mouse hair follicle morphogenesis and cycling

Andrei A. Panteleyev, Ralf Paus, Angela M. Christiano

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The hr (hairless) gene encodes a putative transcription factor with restricted expression in the skin and brain. Mutations in the hr locus cause papular atrichia in humans and complete hair loss in mice and other mammals. To further elucidate the role of hr in skin biology, and to identify potential target cells for hr regulation, we studied hr mRNA localization during hair follicle (HF) morphogenesis and cycling in normal C57BL/6J mice. In situ hybridization revealed that hr expression was present in the suprabasal cell layers of the epidermis, whereas the basal and highly differentiated keratinocytes of the granular layer were hr-negative. During the early stages of HF morphogenesis, hr mRNA was detected in the developing hair peg. Later, it became concentrated in the HF infundibulum, in the HF matrix, and in the inner root sheath (IRS), whereas the dermal papilla (DP) and outer root sheath were consistently hr mRNA-negative. During catagen, hr gene expression gradually declined in the regressing IRS, shortly but dramatically increased in the zone of developing club hair, and became up-regulated in the epithelial cells adjacent to the DP. The co-localization of hr mRNA with the site of the morphological defects in mutant skin implicates hr as a key factor in regulating basic cellular processes during catagen, including club hair formation, maintenance of DP-epithelial integrity, IRS disintegration, and keratinocyte apoptosis in the HF matrix.

Original languageEnglish (US)
Pages (from-to)1071-1079
Number of pages9
JournalAmerican Journal of Pathology
Volume157
Issue number4
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

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Hair Follicle
Morphogenesis
Gene Expression
Skin
Hair
Messenger RNA
Keratinocytes
Alopecia
Pituitary Gland
Inbred C57BL Mouse
Epidermis
In Situ Hybridization
Mammals
Transcription Factors
Epithelial Cells
Maintenance
Apoptosis
Mutation
Brain
Genes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Patterns of hairless (hr) gene expression in mouse hair follicle morphogenesis and cycling. / Panteleyev, Andrei A.; Paus, Ralf; Christiano, Angela M.

In: American Journal of Pathology, Vol. 157, No. 4, 01.01.2000, p. 1071-1079.

Research output: Contribution to journalArticle

Panteleyev, Andrei A. ; Paus, Ralf ; Christiano, Angela M. / Patterns of hairless (hr) gene expression in mouse hair follicle morphogenesis and cycling. In: American Journal of Pathology. 2000 ; Vol. 157, No. 4. pp. 1071-1079.
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