Patterns of CD8 T cell clonal dominance in response to change in antiretroviral therapy in HIV-infected children

M. Kharbanda, S. Than, V. Chitnis, M. Sun, S. Chavan, S. Bakshi, S. Pahwa

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Objective: To examine the influence of change in antiretroviral therapy (ART) on patterns of CD8 T cell clonal dominance in HIV-infected children. Design: Seventeen HIV-infected children with plasma virus loads between 3.1 and 5.7 log10 were investigated before and after changes in ART. Methods: CDR3 spectratyping was performed in 22 T cell receptor (TCR) Vβ sub-families by multiplex polymerase chain reaction (PCR) in purified peripheral blood CD8 T cells in conjunction with CD4 cell counts, plasma HIV-RNA copies and lymphoproliferative assays (LPA). Results: CD8 T cell clonal dominance in two or more Vβ families was present in eight out of 17 children. After a change in therapy, 13 patients (76%) acquired new clones whereas three patients (17.6%) showed a loss in CD8 cell clones. An increase in the numbers of dominant clones correlated with an increase in percentage CD4 cell counts (P<0.001) and with improved LPA responses to tetanus (P<0.05) and alloantigens (P < 0.01). CD4 cell increase was associated with an initial mean gain of 3.1 ± 2.1 CD8 cell clones, independent of a virological response. A loss of CD8 cell clones or failure to achieve CD4 T cell increase was associated with failure to achieve virological suppression. Conclusion: Children with chronic HIV infection manifest CD8 T cell clonal dominance, which appears to be dependent upon the adequacy of the CD4 cells. With optimization of therapy, a gain in clonal dominance is the predominant response, except in situations of failure to contain viral replication. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish (US)
Pages (from-to)2229-2238
Number of pages10
JournalAIDS
Volume14
Issue number15
DOIs
StatePublished - Nov 23 2000
Externally publishedYes

Keywords

  • Antiretroviral therapy
  • CD8 T cells
  • HIV
  • Pediatrics
  • T cell receptor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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