Pattern stability under cell culture conditions - A comparative study of patterning methods based on PLL-g-PEG background passivation

Jost W. Lussi, Didier Falconnet, Jeffrey A. Hubbell, Marcus Textor, Gabor Csucs

Research output: Contribution to journalArticle

77 Scopus citations


Despite the rapidly increasing number of publications on the fabrication and use of micro-patterns for cell studies, comparatively little is know about the long-term stability of such patterns under cell culture conditions. Here, we report on the long-term stability of cellular patterns created by three different patterning techniques: selective molecular assembly patterning, micro-contact printing and molecular assembly patterning by lift-off. We demonstrate that although all three techniques were combined with the same background passivation chemistry based on assembly of a PEG-graft copolymer, there are considerable differences in the long-term stability between the three different pattern types under cell culture conditions. Our results suggest that these differences are not cell-dependent but are due to different (substrate-dependent) interactions between the patterned substrate, the passivating molecule and the serum containing cellular medium.

Original languageEnglish (US)
Pages (from-to)2534-2541
Number of pages8
Issue number12
StatePublished - Apr 1 2006



  • MAPL
  • Microcontact printing
  • Molecular assembly patterning by lift-off
  • Pattern degradation
  • Selective molecular assembly patterning
  • SMAP

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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