Pattern stability under cell culture conditions - A comparative study of patterning methods based on PLL-g-PEG background passivation

Jost W. Lussi, Didier Falconnet, Jeffrey A. Hubbell, Marcus Textor, Gabor Csucs

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Despite the rapidly increasing number of publications on the fabrication and use of micro-patterns for cell studies, comparatively little is know about the long-term stability of such patterns under cell culture conditions. Here, we report on the long-term stability of cellular patterns created by three different patterning techniques: selective molecular assembly patterning, micro-contact printing and molecular assembly patterning by lift-off. We demonstrate that although all three techniques were combined with the same background passivation chemistry based on assembly of a PEG-graft copolymer, there are considerable differences in the long-term stability between the three different pattern types under cell culture conditions. Our results suggest that these differences are not cell-dependent but are due to different (substrate-dependent) interactions between the patterned substrate, the passivating molecule and the serum containing cellular medium.

Original languageEnglish
Pages (from-to)2534-2541
Number of pages8
JournalBiomaterials
Volume27
Issue number12
DOIs
StatePublished - Apr 1 2006
Externally publishedYes

Fingerprint

Phase locked loops
Cell culture
Passivation
Polyethylene glycols
Cell Culture Techniques
Printing
Publications
Graft copolymers
Substrates
Transplants
Serum
Fabrication
Molecules
polylysine-graft-(poly(ethylene glycol))

Keywords

  • MAPL
  • Microcontact printing
  • Molecular assembly patterning by lift-off
  • Pattern degradation
  • Selective molecular assembly patterning
  • SMAP

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Pattern stability under cell culture conditions - A comparative study of patterning methods based on PLL-g-PEG background passivation. / Lussi, Jost W.; Falconnet, Didier; Hubbell, Jeffrey A.; Textor, Marcus; Csucs, Gabor.

In: Biomaterials, Vol. 27, No. 12, 01.04.2006, p. 2534-2541.

Research output: Contribution to journalArticle

Lussi, Jost W. ; Falconnet, Didier ; Hubbell, Jeffrey A. ; Textor, Marcus ; Csucs, Gabor. / Pattern stability under cell culture conditions - A comparative study of patterning methods based on PLL-g-PEG background passivation. In: Biomaterials. 2006 ; Vol. 27, No. 12. pp. 2534-2541.
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