Pattern of colony-stimulating activity in HL-60 cells after phorbol-ester-induced differentiation

S. L. Perkins, Adel A Yunis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

HL-60 cells secrete an autostimulatory activity (ASA) that appears to be required for their growth in vitro. The purpose of this study was to examine the pattern of colony-stimulating activity in conditioned media of HL-60 after induction of differentiation by the phorbol ester phorbol-12-myristate-13-acetate (PMA). PMA-induced differentiation to monocyte-macrophage cells was accompained by persistence of ASA secretion and the appearance of new species of colony-stimulating activities similar in properties to human CSF I and CSF II. Thus the secretion of ASA by HL-60 appears to be a characteristic property of these cells that is maintained after terminal differentiation.

Original languageEnglish (US)
Pages (from-to)401-405
Number of pages5
JournalExperimental Hematology
Volume14
Issue number5
StatePublished - 1986

Fingerprint

HL-60 Cells
Phorbol Esters
Acetates
Conditioned Culture Medium
Monocytes
Macrophages
Growth
phorbol-12-myristate
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Pattern of colony-stimulating activity in HL-60 cells after phorbol-ester-induced differentiation. / Perkins, S. L.; Yunis, Adel A.

In: Experimental Hematology, Vol. 14, No. 5, 1986, p. 401-405.

Research output: Contribution to journalArticle

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