Pathophysiology of neonatal lung injury induced by monoclonal antibody to surfactant protein B

Gertie Grossmann, Yasuhiro Suzuki, Bengt Robertson, Tsutomu Kobayashi, Per Berggren, Wen Zhi Li, Guo Wei Song, Bo Sun

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Near-term newborn rabbits were exposed via the airways to a monoclonal antibody to surfactant protein B and ventilated for 0-120 min. Control animals received nonspecific rabbit or mouse immunoglobulin G, saline, or no material via the airways. Administration of the antibody at ≤40 mg/kg elicited an immediate, significant fall in lung-thorax compliance associated with progressive intra-alveolar edema and/or alveolar collapse and necrosis and desquamation of airway epithelium, and hyaline membranes. The vascular- to-alveolar leak of human albumin and human immunoglobulin G, injected intravenously at birth and determined in lung lavage fluid 60-120 min after instillation of the antibody, was 1.8% for the left lung, with no difference between the markers. The average leak in control animals ventilated for 120 min was <0.3% (P < 0.05). Cytospin preparations of lung lavage fluid from animals exposed to the antibody showed significantly increased recruitment of neutrophilic granulocytes. The pathology and pathophysiology of neonatal lung injury induced by the monoclonal antibody to surfactant protein B probably reflect a combination of direct inactivation of surfactant and an inflammatory response triggered by the immune reaction.

Original languageEnglish
Pages (from-to)2003-2010
Number of pages8
JournalJournal of Applied Physiology
Volume82
Issue number6
StatePublished - Jun 1 1997
Externally publishedYes

Fingerprint

Lung Injury
Surface-Active Agents
Monoclonal Antibodies
Bronchoalveolar Lavage Fluid
Antibodies
Immunoglobulin G
Rabbits
Lung Compliance
Hyalin
Granulocytes
Blood Vessels
Albumins
Edema
Necrosis
Thorax
Epithelium
Parturition
Pathology
Lung
Membranes

Keywords

  • Animals, newborn
  • Electron microscopy
  • Lung compliance
  • Lung permeability
  • Respiratory insufficiency

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Grossmann, G., Suzuki, Y., Robertson, B., Kobayashi, T., Berggren, P., Li, W. Z., ... Sun, B. (1997). Pathophysiology of neonatal lung injury induced by monoclonal antibody to surfactant protein B. Journal of Applied Physiology, 82(6), 2003-2010.

Pathophysiology of neonatal lung injury induced by monoclonal antibody to surfactant protein B. / Grossmann, Gertie; Suzuki, Yasuhiro; Robertson, Bengt; Kobayashi, Tsutomu; Berggren, Per; Li, Wen Zhi; Song, Guo Wei; Sun, Bo.

In: Journal of Applied Physiology, Vol. 82, No. 6, 01.06.1997, p. 2003-2010.

Research output: Contribution to journalArticle

Grossmann, G, Suzuki, Y, Robertson, B, Kobayashi, T, Berggren, P, Li, WZ, Song, GW & Sun, B 1997, 'Pathophysiology of neonatal lung injury induced by monoclonal antibody to surfactant protein B', Journal of Applied Physiology, vol. 82, no. 6, pp. 2003-2010.
Grossmann G, Suzuki Y, Robertson B, Kobayashi T, Berggren P, Li WZ et al. Pathophysiology of neonatal lung injury induced by monoclonal antibody to surfactant protein B. Journal of Applied Physiology. 1997 Jun 1;82(6):2003-2010.
Grossmann, Gertie ; Suzuki, Yasuhiro ; Robertson, Bengt ; Kobayashi, Tsutomu ; Berggren, Per ; Li, Wen Zhi ; Song, Guo Wei ; Sun, Bo. / Pathophysiology of neonatal lung injury induced by monoclonal antibody to surfactant protein B. In: Journal of Applied Physiology. 1997 ; Vol. 82, No. 6. pp. 2003-2010.
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