Pathological-corneas layer segmentation and thickness measurement in OCT images

Amr Elsawy, Giovanni Gregori, Taher Eleiwa, Mohamed Abdel-Mottaleb, Mohamed Abou Shousha

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Purpose: The purpose of this study was to propose a new algorithm for the segmentation and thickness measurement of pathological corneas with irregular layers using a two-stage graph search and ray tracing. Methods: In the first stage, a graph, with only gradient edge-cost, is used to segment the air-epithelium and endothelium-aqueous boundaries. In the second stage, a graph, with gradient, directional, and multiplier edge-cost, is used to correct segmentation. The optical coherence tomography (OCT) image is flattened using the air-epithelium boundary and a graph search is used to segment the epithelium-Bowman’s and Bowman’sstroma boundaries. Then, the OCT image is flattened using the endothelium-aqueous boundary and a graph search is used to segment the Descemet’s membrane. Ray tracing is used to correct the inter-boundary distances, then the thickness is measured using the shortest distance. The proposed algorithm was trained and evaluated using 190 OCT images manually segmented by trained operators. Results: The mean and standard deviation of the unsigned errors of the algorithmoperator and inter-operator were 0.89 ± 1.03 and 0.77 ± 0.68 pixels in segmentation and 3.62 ± 3.98 and 2.95 ± 2.52 μm in thickness measurement. Conclusions: Our proposed algorithm can produce accurate segmentation and thickness measurements compared with the manual operators. Translational Relevance: Our algorithm could be potentially useful in the clinical practice.

Original languageEnglish (US)
Article number24
Pages (from-to)1-17
Number of pages17
JournalTranslational Vision Science and Technology
Issue number11
StatePublished - Oct 2020


  • Cornea
  • Optical coherence tomography (OCT) imaging
  • Ray tracing
  • Segmentation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Ophthalmology


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