Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses

Jinxiong Wei, Jennifer Noto, Elena Zaika, Judith Romero-Gallo, Pelayo Correa, Wael El-Rifai, Richard M. Peek, Alexander Zaika

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The p53 protein plays a central role in the prevention of tumorigenesis. Cellular stresses, such as DNA damage and aberrant oncogene activation, trigger induction of p53 that halts cellular proliferation and allows cells to be repaired. If cellular damage is beyond the capability of the repair mechanisms, p53 induces apoptosis or cell cycle arrest, preventing damaged cells from becoming cancerous. However, emerging evidence suggests that the function of p53 needs to be considered as isoform-specific. Here, we report that the expression pro fi le of p53 can be shifted toward inhibitory p53 isoforms by the pathogenic bacterium Helicobacter pylori, which is known for its strong association with gastric cancer and gastric mucosa-associated lymphoid tissue lymphoma. We found that interaction of H. pylori with gastric epithelial cells, mediated via the cag pathogenicity island, induces N-terminally truncated Δ133p53 and Δ160p53 isoforms in human cells. Induction of an orthologous p53 isoform, Δ153p53, was also found in H. pylori-infected Mongolian gerbils. The p53 isoforms inhibit p53 and p73 activities, induce NF-κB, and increase survival of infected cells. Expression of Δ133p53, in response to H. pylori infection, is regulated by phosphorylation of c-Jun and activation of activator protein-1-dependent transcription. Together, these results provide unique insights into the regulation of p53 protein and may contribute to the understanding of tumorigenesis associated with H. pylori.

Original languageEnglish (US)
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number38
DOIs
StatePublished - Sep 18 2012
Externally publishedYes

Fingerprint

Helicobacter pylori
Protein Isoforms
Bacteria
Stomach Neoplasms
Carcinogenesis
Genomic Islands
Marginal Zone B-Cell Lymphoma
Gerbillinae
Transcription Factor AP-1
Helicobacter Infections
Gastric Mucosa
Cell Cycle Checkpoints
Oncogenes
DNA Damage
Cell Survival
Stomach
Proteins
Epithelial Cells
Phosphorylation
Cell Proliferation

Keywords

  • AP-1
  • c-Jun
  • Gastric tumor

ASJC Scopus subject areas

  • General

Cite this

Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses. / Wei, Jinxiong; Noto, Jennifer; Zaika, Elena; Romero-Gallo, Judith; Correa, Pelayo; El-Rifai, Wael; Peek, Richard M.; Zaika, Alexander.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 38, 18.09.2012.

Research output: Contribution to journalArticle

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