Dose-response studies with des-(pyro) Glu-LH-RH, [Phe3] LH-RH, [Phe5] LH-RH [0-methyl-Tyr5] LH-RH, [Ile7] LH-RH and des-Gly10-LH-RH-ethylamide show concomitant stimulation of adenohypophyseal adenosine 3',5'-monophosphate (cyclic AMP) concentration, and LH and FSH release in rat anterior pituitary gland in vitro. Des-His2-des-Gly10-LH-RH-ethylamide, and inhibitor of the response to LH-RH in vivo, had no effect on cyclic AMP accumulation or on LH release in vitro. The present finding of parallel changes of intracellular cyclic AMP levels and hormonal release with LH-RH analogs having a wide spectrum of biological activity adds strong support for a role of the cyclic nucleotide as mediator of the action of LH-RH in the anterior pituitary gland.
- releasing factors
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism