Parallel evaluation of two promising immunoisolation strategies for beta cell replacement: Conformal coating versus double alginate encapsulation

Teresa De Toni, Aaron A. Stock, Susan A. Safley, Collin Weber, Oscar Alcazar, Peter Buchwald, Alice Tomei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Pancreatic islet transplantation represents a therapeutic option for type 1 diabetes patients to restore insulin secretion and glucose homeostasis. However, chronic immunosuppression is necessary to prevent graft rejection and graft function is difficult to maintain over prolonged times. Encapsulating pancreatic islets with hydrogel biomaterials represents a strategy for immunoisolation, preventing the direct contact between donor islets and recipient immune system. At the same time, hydrogels allow efficient inward diffusion of nutrients, oxygen, and glucose as well as glucose-stimulated release of insulin. Here, we compared two encapsulation technologies that have proven to be effective in preclinical models – Conformal Coating (CC) 1 and Double Capsules (DC) 2 , which are fabricated with different materials: polyethylene glycol (PEG) and alginate, respectively. We evaluated the hydrogel diffusivity properties and the functionality of human islets (HI) in CC and DC through static (GSIR) and dynamic (perifusion) glucose stimulated insulin release assays.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

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    Toni, T. D., Stock, A. A., Safley, S. A., Weber, C., Alcazar, O., Buchwald, P., & Tomei, A. (2019). Parallel evaluation of two promising immunoisolation strategies for beta cell replacement: Conformal coating versus double alginate encapsulation. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.