Abstract
Pancreatic islet transplantation represents a therapeutic option for type 1 diabetes patients to restore insulin secretion and glucose homeostasis. However, chronic immunosuppression is necessary to prevent graft rejection and graft function is difficult to maintain over prolonged times. Encapsulating pancreatic islets with hydrogel biomaterials represents a strategy for immunoisolation, preventing the direct contact between donor islets and recipient immune system. At the same time, hydrogels allow efficient inward diffusion of nutrients, oxygen, and glucose as well as glucose-stimulated release of insulin. Here, we compared two encapsulation technologies that have proven to be effective in preclinical models – Conformal Coating (CC) 1 and Double Capsules (DC) 2 , which are fabricated with different materials: polyethylene glycol (PEG) and alginate, respectively. We evaluated the hydrogel diffusivity properties and the functionality of human islets (HI) in CC and DC through static (GSIR) and dynamic (perifusion) glucose stimulated insulin release assays.
| Original language | English (US) |
|---|---|
| Title of host publication | Society for Biomaterials Annual Meeting and Exposition 2019 |
| Subtitle of host publication | The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting |
| Publisher | Society for Biomaterials |
| Number of pages | 1 |
| ISBN (Electronic) | 9781510883901 |
| State | Published - Jan 1 2019 |
| Event | 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States Duration: Apr 3 2019 → Apr 6 2019 |
Publication series
| Name | Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium |
|---|---|
| Volume | 40 |
| ISSN (Print) | 1526-7547 |
Conference
| Conference | 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence |
|---|---|
| Country | United States |
| City | Seattle |
| Period | 4/3/19 → 4/6/19 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Biotechnology
- Biomaterials
- Materials Chemistry
Cite this
Parallel evaluation of two promising immunoisolation strategies for beta cell replacement : Conformal coating versus double alginate encapsulation. / Toni, Teresa De; Stock, Aaron A.; Safley, Susan A.; Weber, Collin; Alcazar, Oscar; Buchwald, Peter; Tomei, Alice.
Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Parallel evaluation of two promising immunoisolation strategies for beta cell replacement
T2 - Conformal coating versus double alginate encapsulation
AU - Toni, Teresa De
AU - Stock, Aaron A.
AU - Safley, Susan A.
AU - Weber, Collin
AU - Alcazar, Oscar
AU - Buchwald, Peter
AU - Tomei, Alice
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Pancreatic islet transplantation represents a therapeutic option for type 1 diabetes patients to restore insulin secretion and glucose homeostasis. However, chronic immunosuppression is necessary to prevent graft rejection and graft function is difficult to maintain over prolonged times. Encapsulating pancreatic islets with hydrogel biomaterials represents a strategy for immunoisolation, preventing the direct contact between donor islets and recipient immune system. At the same time, hydrogels allow efficient inward diffusion of nutrients, oxygen, and glucose as well as glucose-stimulated release of insulin. Here, we compared two encapsulation technologies that have proven to be effective in preclinical models – Conformal Coating (CC) 1 and Double Capsules (DC) 2 , which are fabricated with different materials: polyethylene glycol (PEG) and alginate, respectively. We evaluated the hydrogel diffusivity properties and the functionality of human islets (HI) in CC and DC through static (GSIR) and dynamic (perifusion) glucose stimulated insulin release assays.
AB - Pancreatic islet transplantation represents a therapeutic option for type 1 diabetes patients to restore insulin secretion and glucose homeostasis. However, chronic immunosuppression is necessary to prevent graft rejection and graft function is difficult to maintain over prolonged times. Encapsulating pancreatic islets with hydrogel biomaterials represents a strategy for immunoisolation, preventing the direct contact between donor islets and recipient immune system. At the same time, hydrogels allow efficient inward diffusion of nutrients, oxygen, and glucose as well as glucose-stimulated release of insulin. Here, we compared two encapsulation technologies that have proven to be effective in preclinical models – Conformal Coating (CC) 1 and Double Capsules (DC) 2 , which are fabricated with different materials: polyethylene glycol (PEG) and alginate, respectively. We evaluated the hydrogel diffusivity properties and the functionality of human islets (HI) in CC and DC through static (GSIR) and dynamic (perifusion) glucose stimulated insulin release assays.
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M3 - Conference contribution
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
ER -