TY - GEN
T1 - Parallel evaluation of two promising immunoisolation strategies for beta cell replacement
T2 - 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
AU - Toni, Teresa De
AU - Stock, Aaron A.
AU - Safley, Susan A.
AU - Weber, Collin
AU - Alcazar, Oscar
AU - Buchwald, Peter
AU - Tomei, Alice
PY - 2019/1/1
Y1 - 2019/1/1
N2 -
Pancreatic islet transplantation represents a therapeutic option for type 1 diabetes patients to restore insulin secretion and glucose homeostasis. However, chronic immunosuppression is necessary to prevent graft rejection and graft function is difficult to maintain over prolonged times. Encapsulating pancreatic islets with hydrogel biomaterials represents a strategy for immunoisolation, preventing the direct contact between donor islets and recipient immune system. At the same time, hydrogels allow efficient inward diffusion of nutrients, oxygen, and glucose as well as glucose-stimulated release of insulin. Here, we compared two encapsulation technologies that have proven to be effective in preclinical models – Conformal Coating (CC)
1
and Double Capsules (DC)
2
, which are fabricated with different materials: polyethylene glycol (PEG) and alginate, respectively. We evaluated the hydrogel diffusivity properties and the functionality of human islets (HI) in CC and DC through static (GSIR) and dynamic (perifusion) glucose stimulated insulin release assays.
AB -
Pancreatic islet transplantation represents a therapeutic option for type 1 diabetes patients to restore insulin secretion and glucose homeostasis. However, chronic immunosuppression is necessary to prevent graft rejection and graft function is difficult to maintain over prolonged times. Encapsulating pancreatic islets with hydrogel biomaterials represents a strategy for immunoisolation, preventing the direct contact between donor islets and recipient immune system. At the same time, hydrogels allow efficient inward diffusion of nutrients, oxygen, and glucose as well as glucose-stimulated release of insulin. Here, we compared two encapsulation technologies that have proven to be effective in preclinical models – Conformal Coating (CC)
1
and Double Capsules (DC)
2
, which are fabricated with different materials: polyethylene glycol (PEG) and alginate, respectively. We evaluated the hydrogel diffusivity properties and the functionality of human islets (HI) in CC and DC through static (GSIR) and dynamic (perifusion) glucose stimulated insulin release assays.
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M3 - Conference contribution
AN - SCOPUS:85065440483
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
Y2 - 3 April 2019 through 6 April 2019
ER -
